- Open Access
Ant genomics sheds light on the molecular regulation of social organization
© BioMed Central Ltd 2013
- Published: 29 July 2013
Ants are powerful model systems for the study of cooperation and sociality. In this review, we discuss how recent advances in ant genomics have contributed to our understanding of the evolution and organization of insect societies at the molecular level.
- Social insect
- Caste determination
- Division of labor
Ants are among the most ecologically diverse and successful animals on our planet. They have colonized most terrestrial environments, where they occupy keystone positions and have strong ecological impacts owing to their crucial roles as scavengers, predators, granivores, herbivores and mutualists . Understanding the ecological dominance of ants requires investigation into the complex organization of their societies.
Summary of key parameters of seven sequenced ant genomes
Depth of coverage
Assembly size (Mb)
Total genome size (Mb)
Contig N50 (bp)
Scaffold N50 (bp)
G+C composition (%)
Orthologs + co-orthologs
Manually curated genes
Genes with EST support (%)
Total repeat content (%)
One of the most striking features of ants is the presence of queens and workers that can differ greatly in morphology, physiology and behavior. In some species, workers (which are all females) can also differ in size and morphology (such as minors, majors and soldiers). In some species, caste determination stems mostly from a developmental switch controlled by environmental factors, whereas, in others, strong genetic effects can also influence the process of caste determination. These genetic influences range from plastic genotypes that are biased toward queen or worker development to a strictly genetic determination . Thus, ants provide an ideal system to investigate how differences in the social environment trigger developmental switches, changes in gene expression and the production of alternative phenotypes.
Recent studies implicate epigenetic processes in caste determination during larval development. Contrary to Drosophila fruit flies and Tribolium flour beetles, all sequenced ant and bee species have a complete set of DNA methylation enzymes . Genome methylation in mammals and plants is widespread and primarily occurs in repetitive, transposable and regulatory elements, whereas it is found mostly in gene bodies in ants and bees, suggesting a fundamentally different function of DNA methylation [16–18]. In the carpenter ant Camponotus floridanus and the jumping ant Harpegnathos saltator, DNA methylation is correlated with caste-specific gene expression and alternative splicing, and patterns of DNA methylation change during development . The genomes of the harvester ant Pogonomyrmex barbatus and the Argentine ant Linepithema humile revealed that annotated genes in the networks that underlie reproductive development, apoptosis and wing polyphenism have fewer CpG sites, the main target sites of DNA methylation, than the genome average [4, 5]. This was interpreted as evidence for elevated rates of methylation in these genes because DNA methylation leads to increased mutation rates and thereby a depletion of CpG sites [19, 20]. By contrast, the opposite pattern was observed in the leaf-cutting ant Atta cephalotes , suggesting that there might be important interspecific variation in how DNA methylation affects the production of alternative phenotypes. This is consistent with the recent finding that ant genomes exhibit distinct genome-wide depletion of observed relative to expected CpG sites . Changes in gene transcription are also associated with histone modifications and changes in chromatin structure between castes and developmental stages in H. saltator and C. floridanus [2, 21]. Both genome methylation and histone modification might therefore influence caste determination through transcriptional control and alternative splicing.
A recent comparative analysis suggested that the abundance and diversity of transcription factor binding sites (TFBSs) might also play a role in the evolution of caste-specific patterns of gene expression . TFBSs were found to be more divergent within ants than between social and solitary insects, and genes exhibiting important changes in the abundance of TFBSs between social and solitary insects showed higher levels of gene expression plasticity between castes in C. floridanus (high polymorphism and reproductive division of labor) compared with H. saltator (low polymorphism and reproductive division of labor). Furthermore, the ant genomes revealed that the networks commonly known to exhibit phenotypic plasticity between castes (such as the neuroendocrine system) were preferentially targeted for regulatory changes during the evolution of sociality .
The genome sequences also facilitate the identification and study of candidate genes in the process of caste differentiation [5–7, 20]. For example, analysis of insulin signaling, juvenile hormone and vitellogenin expression during artificial hibernation and hormone manipulation revealed how the environmental cues experienced by Pogonomyrmex rugosus queens are translated into the production of new queens and workers . Similarly, the fire ant (Solenopsis invicta) was found to harbor two insulin receptors, which might play a role in the process of caste determination . Finally, a study on molecular evolution in S. invicta revealed a positive association between caste-biased gene expression and the rate of gene evolution, the latter being driven largely by variation in the strength of purifying selection . This study also showed that high rates of gene evolution actually preceded gene expression bias associated with the evolution of castes, suggesting that fast-evolving genes are more likely to be recruited to the processes underlying phenotypic plasticity .
Genetic effects on morphological caste determination have been found in L. humile  and the leaf-cutting ant Acromyrmex echinatior [26, 27]. An extreme case of genetic caste determination occurs in the genus Pogonomyrmex, where some populations contain differentiated genetic lineages, most or all of which derive from historical hybridization between the harvester ants P. rugosus and P. barbatus [28–32]. These lineages always occur in pairs , and queens in each lineage-pair mate multiple times with males of their own as well as with males of the alternative lineage. Inter-lineage offspring develop into workers, whereas intra-lineage offspring develop into queens. Thus, the only way that a queen can produce a colony with both workers and queens is by mating with males of both lineages. Crossing experiments revealed that intra-lineage individuals are developmentally constrained to become queens . Inter-lineage individuals have partly retained plasticity and can develop into queens under some conditions, but the association of genotype and caste is very strong, with almost no adult females presenting a mismatch between the genotype and expected phenotype . This provides an interesting system to compare the epigenetic and gene expression changes across developmental stages between individuals for which caste fate is already known .
In addition to the reproductive division of labor between queens and workers, there is also usually a strong division of labor between workers, which specialize in different tasks. Worker behavior is influenced by several factors, including size and morphology [35–37], age [38, 39], individual experience [40, 41] and genetic background [42, 43]. Worker behavior and task specialization in ants are often regulated by genes that also affect behavior in solitary insects (the foraging gene and circadian clock genes, for example) [44–48].
Reproduction and behavior are often interconnected in animals. In solitary wasps, for instance, females with developed ovaries lay eggs, whereas females with undeveloped ovaries forage for food. Accordingly, studies in the honeybee Apis mellifera suggest that physiological pathways regulating reproduction and behavior in solitary insects have been co-opted for the regulation of worker behavior in social insect species [49, 50]. Studies in the ant Pogonomyrmex californicus revealed that nurses and foragers differ in ovary activity  and juvenile hormone levels , which are known to affect the production of vitellogenin (typically involved in reproduction) . Interestingly, phylogenetic analyses revealed the existence of multiple genes encoding vitellogenin in most of the ant genomes sequenced , suggesting that an initial duplication of the ancestral vitellogenin gene occurred after ants diverged from bees and wasps. The ant vitellogenin genes cluster in two paralogous gene families that show caste- and behavior-specific expression in S. invicta  and P. rugosus , suggesting that vitellogenin in ants not only regulates reproduction but also the behavior of sterile workers . The finding that vitellogenin plays similar roles in ants and bees, which evolved sociality independently, supports the hypothesis that the co-option of reproductive pathways plays a major role in social evolution.
Effective communication is an important ingredient for any society to function efficiently. Ants rely heavily on chemical communication for social organization and to discriminate nestmates from non-nestmates . The importance of chemical communication in ants is reflected by large expansions of chemosensory gene families and metabolic pathways for cuticular hydrocarbons in all ant genomes that have been studied in this respect compared with other sequenced hymenopterans [2, 4, 5, 7, 54]. A comparative analysis of antennal transcriptomes between C. floridanus and H. saltator identified many chemoreceptors differentially expressed between males and females, as well as between species, suggesting that sex- and species-specific biology is likely to have shaped the expression patterns of genes involved in communication .
A recent study of chemosensory protein genes revealed contrasting modes of evolution between genes occurring only in ants and those also found in the honeybee . Clades with ant-specific expansions showed evidence of faster evolution and elevated levels of positive selection compared with clades of one-to-one orthologs across all ants and the honeybee. A possible explanation is that the more conserved chemosensory protein genes that occur in both ants and bees are associated with more general features of social insect biology, whereas genes in ant-specific expansions might be related to more idiosyncratic environmental and social conditions . At the same time, ant-specific desaturase gene families, which are involved in the production of chemical signals, show an elevated number of genes and high variability in both sequence and expression, possibly reflecting an increased demand for diversity in the chemical signals used in ant communication .
Analysis of the seven sequenced ant genomes has already led to significant advances in our understanding of important aspects of ant biology. Below, we highlight three avenues of research that we believe will prove fruitful as this endeavor continues.
First, large comparative analyses of the genomes of ants, social bees and social wasps (which evolved sociality independently) with the genomes of solitary bees, wasps and other insects will be needed to investigate the key evolutionary changes associated with sociality in the Hymenoptera. The only study that has conducted such a comparative analysis so far has provided valuable information on the evolution of several aspects of social organization (caste determination, chemical communication and social immunity), as discussed above .
A second important step will be to perform functional studies to validate experimentally the numerous hypotheses generated by comparative analyses. So far, only a handful of studies have manipulated gene expression in ants using RNA interference [74, 75], hormonal treatments [22, 76] or pharmacological manipulations , and none of them investigated the consequences at the genome scale. Forward genetics, using, for example, random mutagenesis, has been prohibitive in ants and other social insects because these approaches require substantial subsequent crossing. This is not feasible in ants mainly owing to their long generation times and the difficulty to breed most species in the laboratory. However, reverse-genetic approaches for social insects using new and highly effective tools for targeted genome editing have now come within reach. These approaches use engineered nucleases, such as zinc-finger nucleases (ZFNs) , transcription activator-like effector nucleases (TALENs)  or CRISPR RNA-guided Cas9 nucleases [79, 80]. Although these techniques will not be able to overcome the fundamental experimental limitations posed by many ant species, they are now opening up the possibility of creating transgenics and genetic knockouts for a subset of carefully chosen model species that can be propagated in the laboratory. Studies using combinations of genetic, pharmacological, social, hormonal and pheromonal manipulations will be necessary to provide a better understanding of the actual roles of different genes and physiological pathways in regulating ant social life.
Finally, understanding the molecular organization of ant societies will require precise behavioral data at the individual level to investigate the links between communication, gene expression, physiology and behavior. Collecting such data has long been a difficult task, but a new automated tracking system now allows the automatic quantification of all social interactions between all individuals in an ant colony over the course of several weeks. Using this system has already demonstrated the importance of spatial distribution in the regulation of age-related division of labor in insect societies . The use of such sophisticated behavioral tracking and quantification, combined with next-generation genomic data, could well provide the next answers to the big questions in the biology of social insects.
In conclusion, social insects have played a central role in our understanding of the organization and behaviors of complex animal societies, principally from ethological and ecological perspectives. The advent of next-generation sequencing techniques now provides opportunities to use social insects to study how genetic and environmental contributions interact to control societal organization.
We thank Sean McKenzie for comments on the manuscript. This work was supported by a Marie Curie postdoctoral fellowship to RL, a Leon-Levy postdoctoral fellowship to PRO, grant 1DP2GM105454-01 from the NIH to DJCK and several grants from the Swiss National Science Foundation and an ERC advanced grant to LK.
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