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Table 2 W-chromosome-specific repeats of S. mansoni

From: Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma mansoni and their possible role in sex chromosome emergence

GenBank accession number

Name

Length (bp)

Percentage female hits

Repeat familya

Transcription evidence

FISH localization

Copy number estimateb

Reference

U12442

SMAlphafem-1

338

99.86

SMAlpha retroposon

RT-PCR

p-arm

60,000 - 70,000

[61, 62]

J04665

W1

482

100

Retro

 

Middle of q-arm at frontier between heterochromatin and euchromatin as satellite, middle of q-arm

500

[26, 27]

U10109

W2

715

100

   

400

[62]

HQ880214

W3

786

100

LTR, highly similar to W2, highly similar to R = 407

No transcription (RT-PCR)

As satellite in the middle of q-arm at frontier between heterochromatin and euchromatin or also in the pericentromeric region

200

 

HQ880209

W4

1132

99.52

Highly similar to R = 879

RT-PCR

Same location as W1

800

 

HQ880217

W5

1129

99.27

LTR, similar to Perere-2, identical to R = 564

EST and RT-PCR

Either at the frontier of heterochromatin and euchromatin of the q-arm or in the pericentromeric region, or at both locations

  

HQ880215

W6

310

99.88

Retro

EST

In the pericentromeric region

  

HQ880210

W7

1000

100

DNA transposon, 97% identical to GenBank accession number XP_002570219 (hypothetical protein Smp_186230)

 

In the pericentromeric region

  

HQ880218

W8

266

99.97

Tandem repeat (previously described as TR266), DNA transposon

 

Either at the frontier of heterochromatin and euchromatin of the q-arm or in the pericentromeric region

  

HQ880211

W9

803

100

LINE2, similar to SjR2 retrotransposon

EST

   

HQ880212

W10

682

100

LTR

    

HQ880213

W11

376

100

LINE, similar to R = 170

    

HQ880216

W12

264

100

Retro, 97 to 100% identical to several hypothetical S.m. proteins

    

HQ880219

W13

258

100

Retro

 

In the middle of the heterochromatic part of the q-arm as satellite

  

HQ880220

W14

209

100

DNA transposon, similar to R = 170

    

HQ880221

W15

185

96.62

DNA transposon

    

HQ880222

W16

164

100

Similar to R = 116

    

HQ880223

W17

160

100

LTR

    

HQ880224

W18

160

100

LTR

EST

   

HQ880225

W19

139

100

Retro, 100% identical to GenBank accession number XP_002569391 (hypothetical protein Smp_181820)

EST

   

HQ880226

W20

138

100

     

HQ880227

W21

138

99.84

DNA transposon, similar to R = 116

    

HQ880228

W22

132

100

DNA transposon

    

HQ880229

W23

125

100

DNA transposon

    

HQ880230

W24

115

99.56

Retro, similar to Sh122 repeat and R = 31

    

HQ880231

W25

112

99.65

LTR

    

HQ880232

W26

111

100

DNA transposon

    

HQ880233

W27

110

100

DNA transposon, similar to R = 133 and Sh microsatellite C2

    

HQ880234

W28

108

100

Similar to Sh microsatellite C140

    

HQ880235

W29

97

100

Similar to Sb Sbov20 repeat

    

HQ880236

W30

96

99.05

LTR

    

HQ880237

W31

92

100

LTR

    

HQ880238

W32

89

96.88

DNA transposon

    

HQ880239

W33

86

99.86

LTR

    

HQ880240

W34

82

100

DNA transposon

    

HQ880241

W35

80

100

Retro

EST

   
  1. Because of the WZ-type chromosome set of females, these repeats are female-specific. aCombined Censor, Blast, Teclass results. bqPCR based. LINE, long interspersed element; LTR, long terminal repeat.