- Protein family review
The ADAR protein family
Genome Biology volume 13, Article number: 252 (2012)
Adenosine to inosine (A-to-I) RNA editing is a post-transcriptional process by which adenosines are selectively converted to inosines in double-stranded RNA (dsRNA) substrates. A highly conserved group of enzymes, the adenosine deaminase acting on RNA (ADAR) family, mediates this reaction. All ADARs share a common domain architecture consisting of a variable number of amino-terminal dsRNA binding domains (dsRBDs) and a carboxy-terminal catalytic deaminase domain. ADAR family members are highly expressed in the metazoan nervous system, where these enzymes predominantly localize to the neuronal nucleus. Once in the nucleus, ADARs participate in the modification of specific adenosines in pre-mRNAs of proteins involved in electrical and chemical neurotransmission, including pre-synaptic release machineries, and voltage- and ligand-gated ion channels. Most RNA editing sites in these nervous system targets result in non-synonymous codon changes in functionally important, usually conserved, residues and RNA editing deficiencies in various model organisms bear out a crucial role for ADARs in nervous system function. Mutation or deletion of ADAR genes results in striking phenotypes, including seizure episodes, extreme uncoordination, and neurodegeneration. Not only does the process of RNA editing alter important nervous system peptides, but ADARs also regulate gene expression through modification of dsRNA substrates that enter the RNA interference (RNAi) pathway and may then act at the chromatin level. Here, we present a review on the current knowledge regarding the ADAR protein family, including evolutionary history, key structural features, localization, function and mechanism.
Gene organization and evolutionary history
Adenosine deaminases acting on RNA (ADARs) are enzymes that catalyze the chemical conversion of adenosines to inosines in double-stranded RNA (dsRNA) substrates. Because the properties of inosine mimic those of guanosine (inosine will form two hydrogen bonds with cytosine, for example), inosine is recognized as guanosine by the translational cellular machinery . Adenosine-to-inosine (A-to-I) RNA 'editing,' therefore, effectively changes the primary sequence of RNA targets.
These enzymes, discovered over 25 years ago , are highly conserved in metazoa , although the number of genes and isoforms varies between species. Mammalian genomes encode three ADARs: ADAR1 and ADAR2, which are catalytically active , and ADAR3, which is thought to be catalytically inactive . The Caenorhabditis elegans genome encodes two genes, CeADR1 and CeADR2 , while only a single adar locus is present in the Drosophila genome  (Figure 1a). In addition, the squid  and hydra (RA Reenan, unpublished results) genomes each encode a single adar locus, while the chicken and zebrafish genomes encode two and four adar genes, respectively . Furthermore, ADAR genes are also present in the genomes of both sea urchin and sea anemone, suggesting an early origin of RNA editing enzymes in metazoan evolution . In contrast, ADAR genes do not appear to be present in fungal, plant and yeast genomes .
Interestingly, although prokaryotic genomes do not contain ADAR genes, they do encode a transfer RNA (tRNA) adenosine deaminase (TadA), which modifies specific tRNAs . Eukaryotic orthologs of this RNA editing enzyme, adenosine deaminases acting on tRNAs (ADATs), are also conserved in metazoa and catalyze the deamination of specific adenosines to inosines at or adjacent to the tRNA anticodon . Sequence homology between the catalytic domains of ADARs and ADATs suggests a model in which tRNA modifying enzymes are ancestral to ADARs (Figure 1b). In this model, a duplicate ADAT gene acquired one or more dsRNA binding domains that allowed the protein to recognize and bind dsRNAs. This novel gene is thought to have conferred selective advantage due to repair of detrimental genomic mutations by A-to-I modifications at the mRNA level .
Characteristic structural features
ADAR enzymes share a common domain architecture consisting of a variable number of amino-terminal dsRNA binding domains (dsRBDs) and a carboxy-terminal catalytic deaminase domain  (Figure 1a). Human ADARs possess two or three dsRBDs, while the C. elegans enzymes contain one or two. The single Drosophila ADAR contains two dsRBDs, similar to the mammalian ADAR2. In squid two ADAR enzymes are generated via splicing from a single adar locus: while isoform 2a contains two dsRBDs, the inclusion of an alternative exon leads to the generation of an ADAR enzyme (2b) containing an additional dsRBD, which confers higher enzymatic activity in vitro . Interestingly, recent evidence suggests that this extra dsRBD of the squid ADAR isoform 2b is required for appropriate RNA editing in the high salt conditions of a marine environment , suggesting a link between the regulation of the RNA editing process and changes in the physical environment. In agreement with this, additional evidence suggests that RNA editing may be regulated by temperature [13, 14]. Finally, the Hydra magnapapillata genome encodes an ADAR enzyme that contains five identifiable dsRBDs (Figure 1a; RA Reenan, unpublished results).
A single domain feature is unique to human ADAR1, which contains two Z-DNA binding domains  that recognize the left-handed helical variant of DNA in a sequence-independent manner . In fact, the Z-DNA binding domain was first identified in human ADAR1 (hADAR1) , and prior to this discovery Z-DNA was thought to be non-functional . Interestingly, binding of hADAR1 to Z-DNA restricts the nucleic acid from adopting another DNA conformation . This restriction is thought to play an important role in gene expression, as the binding of ADAR to Z-DNA around a reporter gene promoter results in increased levels of transcription . However, more work is needed in this area to understand the specific function of the unique Z-DNA binding domain in hADAR1.
The crystal structure of the human ADAR2 deaminase domain (Figure 2) suggests that a catalytic center forms in the enzyme active site: a glutamic acid residue (E396) forms hydrogen bonds with a water molecule, while a histidine (H394) and two cysteine residues (C451 and C516) coordinate a zinc ion that activates the water molecule for nucleophilic attack . Buried in the catalytic core, an inositol hexakisphosphate (IP6) molecule stabilizes multiple arginine and lysine residues and is also required for catalytic activity .
Localization and function
ADARs localize to the nucleus
The three mammalian Adar genes give rise to four known isoforms: ADAR1p150, ADAR1p110, ADAR2 and ADAR3. ADAR1 variants and ADAR2 are expressed in many tissues, whereas the ADAR3 protein is only present in the brain [5, 21, 22]. ADAR3 is thought to be catalytically inactive , but it is possible that it competes with ADAR1 and -2 for RNA binding substrates, altering the editing profile through that mechanism. Alternative promoter usage within the ADAR1 transcript generates a full length (ADAR1p150) and an amino-terminally truncated (ADAR1p110) isoform , both of which contain three dsRNA-binding domains and the deaminase domain.
The ADAR1 isoforms differ in their cellular distributions: ADAR1p110 exclusively localizes to the nucleus [24, 25], while ADAR1p150 shuttles in and out of the nucleus, although it accumulates in the cytoplasm [23, 26]. The cytoplasmic localization of ADAR1p150 suggests that a select class of dsRNAs could be targeted outside the nucleus as mature mRNAs. However, cytoplasmic localization of other ADARs is unusual.
In general, mammalian ADARs are strongly localized to the nucleus. More specifically, ADAR1p110 and ADAR2 are present in the nucleolus and this localization is dependent on functional dsRBDs [24, 25]. ADAR nucleolar localization may be mediated by dsRNAs generated from ribosomal RNAs (rRNAs) and small nucleolar RNAs (snoRNAs), both of which are concentrated within the nucleolus. To date there is no evidence that ADAR modification of rRNAs or snoRNAs occurs; thus, the significance of ADAR nucleolar localization remains enigmatic. However, translocation of ADAR2 from the nucleolus to the nucleoplasm upon expression induction of a known editing target resulted broadly in higher editing levels in pre-mRNA substrates . Therefore, the nucleolar localization of ADARs may represent a mechanism for regulating editing of pre-mRNAs through enzyme sequestration.
In Drosophila, Jepson et al.  examined the complete expression pattern and cellular distribution of dADAR using a genetically engineered allele containing an HA epitope tag inserted into the endogenous locus. Visualization of dADAR-HA revealed that Drosophila ADAR is predominately expressed in the nervous system. Furthermore, both transgenic dADAR specifically expressed in the salivary glands of third instar larvae, as well as endogenous tagged dADAR, localize within the nucleus and accumulate within the nucleolus  (Figure 3a,b), consistent with observations regarding mammalian ADARs.
Specific ADAR modification can recode dsRNA targets
Electropherograms derived from edited cDNAs include mixed A/G peaks representative of transcript editing (Figure 4a). The comparison of cDNA libraries to genomic sequences led to the surprising discovery of a multitude of new RNA editing sites in various genetic models. In Drosophila, Hoopengardner et al.  uncovered an intriguing phylogenetic signature of A-to-I RNA editing using a comparative genomic approach. This led to the identification of approximately 50 new editing sites in 16 different genes, all of which encode proteins involved in electrical and chemical neurotransmission. However, to further understand the biological significance of RNA editing a more complete analysis of the inosinomes of multiple organisms is required.
The recent advent of deep sequencing technologies has allowed the identification of new RNA editing sites in several genetic organisms and provided us with a clearer picture regarding the regulation, editing patterns, and biological significance of RNA editing . For example, a recent study in Drosophila using deep sequencing technology led to the identification of 972 novel editing sites in 561 genes involved, not only in neurotransmission, but in a wide range of other functions, suggesting that RNA editing events in coding regions are more widespread than previously anticipated .
While editing in Drosophila often appears to result in genomic recoding, a recent deep sequencing study in humans reported that the majority of RNA editing occurs in non-coding regions of the transcriptome . Thus, the use of nascent deep sequencing technologies to study RNA editing has already uncovered diverse ADAR substrates and revealed variation within RNA editing systems across a broad range of organisms.
The structural diversity of potential RNA substrates results in a wide repertoire of ADAR targets [3, 32]. ADARs recognize short, imperfect RNA duplexes and deaminate select adenosines (specific RNA editing) . In contrast, promiscuous editing of multiple adenosines, also known as 'hyper-editing', usually occurs in long, perfectly paired RNA duplexes.
Imperfectly duplex double-stranded editing substrates are sometimes generated within exonic sequences when the nascent transcript folds, creating simple hairpins [33–36]. Yet, more often intronic sequences with extensive complementarity to upstream or downstream exons containing the targeted adenosine(s) can pair with the exonic sequence to form structurally diverse RNA duplexes. These intronic cis elements, also known as editing site complementary sequences (ECSs), can form simple exon-intron hairpin structures [34, 37–40] or more complex RNA secondary structures such as pseudoknots  (Figure 3c).
The majority of ECS-mediated editing substrates contain mismatches, bulges and loops. It is thought that the presence of such structural imperfections directs ADARs to specific locations on the RNA duplex without the requirement of primary sequence recognition. However, upon binding, ADARs do exhibit sequence preferences for modifying select adenosines over others. In vitro studies using synthetic editing substrates revealed that ADAR enzymes preferentially target adenosines neighbored by a 5' uridine, while adenosines with a 5' guanosine neighbor are exceedingly rare [42, 43]. In addition, adenosines found in mismatches with cytosines are edited more often when compared to other adenosines .
Specific RNA editing often leads to transcript recoding. Because inosine shares the base pairing properties of guanosine, the translational machinery interprets edited adenosines as guanosine, altering the triplet codon, which can result in amino acid substitutions in protein products. More than half the triplet codons in the genetic code could in theory be reassigned through RNA editing . Due to the degeneracy of the genetic code, RNA editing can cause both silent and non-synonymous amino acid substitutions. However, statistically, RNA editing in Drosophila predominantly results in non-synonymous changes  and thus favors the diversification of protein products. While the functional consequences of most specific recoding events are currently unknown, myriad studies in diverse model organisms indicate that specific RNA editing of certain mRNAs can result in profound changes in protein function [33, 46–49].
General transcript analysis reveals RNA editing sites as mixed A/G peaks in electropherograms, but this representation conceals specific transcript-to-transcript variability. Several studies focused on transcripts with multiple editing sites, which showed that completely edited and unedited mRNA transcripts co-exist within cells. For example, examination of editing patterns in the Drosophila potassium channel, shaker (sh), revealed that unedited and edited transcripts co-exist and their relative proportions change in a spatial, temporal and sexually dimorphic manner . In addition, the Drosophila paralytic (para) sodium channel transcript, edited at three nearby sites in exon 19, is contemporarily present in both completely edited and unedited forms, as well as in the many combinations of edited isoforms at the three sites .
RNA editing affects splicing
Adenosines targeted for editing are disproportionately localized near splice junctions in the pre-mRNA . Therefore, during formation of a dsRNA ADAR substrate, intronic cis-acting sequences can form RNA duplexes encompassing splicing sites and potentially obscuring them from the splicing machinery (Figure 3c). Furthermore, through modification of select adenosines, ADARs can create or eliminate splicing sites, broadly affecting later splicing of the transcript. Similar to the translational machinery, the spliceosome interprets inosine as guanosine, and therefore, a canonical GU 5' splice site and AG 3' acceptor site can be created via the deamination of AU (IU = GU) and AA (AI = AG), respectively . Correspondingly, RNA editing can destroy a canonical AG 3' splice site (IG = GG) .
For example, mammalian ADAR2 edits its own transcript (auto-editing), leading to the generation of a novel 3' splice site. Auto-editing results in the inclusion of 47 nucleotides  (Figure 3d), causing a frameshift and leading to a decrease in overall ADAR2 protein . The regulation of Adar2 transcript splicing via auto-editing represents a negative regulatory feedback mechanism to modulate ADAR2 protein levels since inhibition of auto-editing in vivo increases ADAR2 expression and editing at many target adenosines in the transcriptome .
Yet even editing at sites that do not encode splicing signals can affect splicing. The mammalian GluR-2 transcript, for example, is edited at two non-synonymous sites: the 'Q/R' and 'R/G' sites, named for the residue changes resulting from editing. When the Q/R site, located in exon 11, as well as a downstream intronic 'hot spot,' is edited, splicing is enhanced between exons 11 and 12 (Figure 3d). In the same transcript, the R/G site is located just one nucleotide from the boundary between exon 13 and the downstream intron (Figure 3c). When this site is edited, splicing favors inclusion of exon 15 over that of exon 14 (Figure 3d). The mechanism of how these particular editing sites affect splicing of the GluR-2 transcript is still unknown .
Regulation of siRNA/miRNA biogenesis and function through RNA editing
Deamination of adenosines found in non-coding regions influences the biogenesis and target recognition of small interfering RNAs (siRNAs) involved in the RNA interference (RNAi) pathway . The biogenesis of siRNAs requires processing of long dsRNA precursors into 21- to 23-nucleotide RNA duplexes, a task carried out by the Dicer class of RNase III-like ribonuclease enzymes . The mature siRNAs generated by Dicer are unwound for the incorporation of a single-stranded RNA ('guide' RNA) into the RNA-induced silencing complex (RISC). Ultimately, the targeting of RISC by the small guide RNA initiates transcriptional and post-transcriptional sequence-specific silencing .
Growing evidence supports the interaction between the editing and RNAi pathways. Since the RNA editing and RNAi pathways both involve dsRNAs, editing is theoretically capable of antagonizing the RNAi pathway at two levels. First, adenosine deamination could alter the perfectly duplex structure of dsRNA substrates, leading to poor Dicer processing and thus decreased siRNA concentrations (Figure 3e). Second, RNA editing of siRNAs may prevent base pairing of the RISC with the original mRNA targets, inhibiting the cleavage step of RNAi . Similarly, editing would change the target of an siRNA if ADAR edits the primary sequence of the guide RNA. siRNA biogenesis is progressively inhibited with increased RNA editing in vitro [57, 58]; however, the in vivo functional consequences of the antagonism between editing and RNAi pathways are still unclear and this remains an open avenue for future ADAR research.
Similar to the siRNA pathway, micro RNAs (miRNAs) are highly conserved, genomically encoded 20- to 25-nucleotide RNAs that mediate gene silencing and regulate diverse cellular processes, including development, differentiation, and apoptosis [59, 60]. This class of small interfering RNAs is encoded within non-coding regions of the genome and form hairpin primary miRNA structures (pri-miRNAs), which are extensively processed to generate the mature miRNA. In their double-stranded state, these pri-miRNAs can serve as ADAR substrates (Figure 3e).
Indeed, several miRNA precursors undergo A-to-I RNA editing at specific adenosines [61, 62]. Editing of these precursors can inhibit further cleavage and processing , as well as regulation of gene expression. A single edited adenosine within the six-nucleotide targeting 'seed' region of the miRNA is sufficient to redirect silencing to a new target  (Figure 3e). In addition, A-to-I RNA editing is highly enriched at 3' UTRs within miRNA targets in multiple mouse tissues, which might represent a regulatory role for RNA editing in translation . More importantly, RNA editing can inhibit or initiate miRNA/target interactions via modification at important positions where complementarity is required for appropriate miRNA targeting.
A-to-I RNA editing of Aluelements
In primates, non-specific A-to-I editing predominantly occurs in Alu elements [66, 67] localized within the introns of transcribed genes. Alu transposable elements are mobile, short DNA sequences that constitute 10% of the human genome . When nearby Alus are inserted into the genome in reverse orientation, their sequences may form a near perfect long duplex when transcribed, an ideal substrate for hyper ADAR modification. This non-specific editing is believed to diversify the transcriptome and some have even proposed that this contributed to increased brain function in primates . Editing in these widespread genomic elements can result in the posttranscriptional creation of splicing signals, leading to exonization and transcript diversification .
For example, A-to-I editing in the human nuclear prelamin A recognition factor generates a novel 3' splice site and alters splicing enhancer signals . Furthermore, ADAR1 small RNA-mediated knockdown in human cells results in the aberrant exonization of Alu elements in a seryl-tRNA synthetase gene, suggesting that RNA editing could globally regulate Alu exonization events within the transcriptome . Alu editing is also necessary for the tissue-specific inclusion of Alu elements in coding sequences .
The binding of an ADAR enzyme to an RNA substrate requires direct contact between the dsRBDs and dsRNA substrates . Nuclear magnetic resonance and X-ray crystallography studies reveal that the binding of double-stranded RNA binding proteins (dsRBPs) in general to dsRNA relies less on sequence than on structure [73–75]. Because dsRNA adopts an A-helical structure, which contains a deep and narrow major groove, dsRNA binding proteins are thought to recognize substrates through indirect readout and RNA structure .
Consistent with the binding properties of other dsRBPs, ADARs can bind to any dsRNA without sequence specificity . It has been widely assumed in the field that the dsRBDs of ADAR enzymes confer structural specificity during specific editing, determining which substrates are targeted and which adenosines are edited. Yet recent in vitro work by Eggington et al.  suggests that mammalian ADAR1 and ADAR2 are both able to edit when lacking amino-terminal dsRBDs. Not only do these truncated enzymes effectively edit in vitro, but they are also able to selectively deaminate the same adenosines targeted by the full-length enzyme, although to different levels. The dsRBDs may function as RNA 'anchors' , but these data suggest that ADAR specificity is predominantly conferred, not by dsRBDs, but by the deaminase domain itself . However, experiments in an in vivo setting are required for the biological relevance of this observation to be understood.
Generation of ADAR deficiencies in genetic model systems highlights an important role for these enzymes in the appropriate functioning of the nervous system. In the last decade several studies have provided a link between RNA editing and multiple nervous system disorders. Abnormal RNA editing is associated with suicidal depression, schizophrenia, epilepsy and amyotrophic lateral sclerosis (ALS) . Furthermore, genetic studies suggest that several mutations in the human ADAR1 gene are associated with dyschromatosis symmetrica hereditaria (DSH) , a rare autosomal skin pigmentation disorder, and Aicardi-Goutières syndrome (AGS) , an early-onset encephalopathy that often results in severe and permanent neurological damage. Thus, a better mechanistic and functional understanding of the ADAR protein family will shed light into therapeutic strategies regarding these human diseases.
ADARs modify specific adenosines to inosines in short imperfect dsRNA templates, a well-characterized role that usually leads to recoding of transcripts and peptides (Figure 4a). In contrast, ADAR modification of long, perfect duplexes results in the deamination of up to 50% of the adenosines [83–85] affecting siRNA biogenesis and activity (Figure 4b). Although much progress has been achieved in ADAR biology over the past two decades, the consequences of such promiscuous RNA editing, especially in vivo, are not well understood.
Several lines of evidence indicate that promiscuous editing by ADARs antagonizes RNAi-mediated gene silencing [32, 86]. Editing of endogenous siRNAs in Drosophila  suggests that ADARs interact with endogenous RNAi pathways, which are involved in somatic defense against transposable elements [87–90] and in the regulation of chromatin states  (Figure 4b). The consequences, however, of editing/RNAi interactions and the resulting effects on both gene expression and chromatin regulation remain enigmatic. Future studies should aim in deciphering the in vivo consequences of such interactions, as this avenue highlights a more global role for ADARs regarding the broad regulation of neuronal transcriptomes.
It was originally proposed that RNA editing evolved to correct detrimental genomic mutations. Although some specific editing sites in the Shab potassium channel are widely conserved across insect species that represent more than 300 million years of independent evolution, other less-conserved sites are genomically encoded in the orthologous genes of species as divergent as squid and insects . These authors also made the striking observation that over half of the editing sites in the squid Kv1 channel transcript are genomically encoded in other species, data that point to a function of editing as a higher order fix for genomic mutations.
However, while editing often occurs in highly invariant exonic regions, it appears to preferentially target non-conserved positions, suggesting that diversity at these locations is so important that it is achieved through both genomic mutation and RNA editing. Yang et al.  suggest that RNA editing performs two functions: to diversify the proteome by generating novel non-genomically encoded proteins, as well as to safeguard important sites of conservation. Indeed, this dual function is consistent with years of observation that include a few editing sites that are genomically encoded in orthologs  as well as myriad unique non-silent editing sites that do not exist in paralogs or appear to restore an ancestral sequence.
Yet the majority of editing sites appear to be non-restorative and therefore it is now widely accepted in the field that the primary role of RNA editing is to increase the diversity of transcripts produced from the more invariant genome, thereby increasing protein repertoire. But what is the functional consequence of increased proteome diversity? RNA editing is most functional within nervous systems and generates novel ion channel properties [33, 94–96]; thus, it is not surprising that mutations in the adar locus lead to defects in complex behaviors in adult Drosophila [14, 27]. In addition, employing a reporter gene for editing activity expressed in the Drosophila nervous system, Jepson et al.  demonstrated that patterns of neuronal RNA editing differ between individuals, implicating the ADAR protein family as a driving force for individual-to-individual variation on both a cellular and organismal scale. Furthermore, manipulation of the firing properties of a single neuron affects global organismal behavior [98, 99]. Thus, it is not a stretch to suggest that ADAR, through the various pathways discussed above, could represent a molecular mechanism for generating variation within a population of cells, resulting in neuronal plasticity and affecting behavior.
adenosine deaminase acting on RNA
adenosine deaminases acting on tRNAs
dsRNA binding domain
editing site complementary sequence
RNA-induced silencing complex
small interfering RNA
Basillo C, Wahba A, Lengyel P, Speyer J, Ochoa S: Synthetic polynucleotides and the amino acid code. V Proc Natl Acad Sci USA. 1962, 48: 613-616. 10.1073/pnas.48.4.613.
Bass BL, Weintraub H: A developmentally regulated activity that unwinds RNA duplexes. Cell. 1987, 48: 607-613. 10.1016/0092-8674(87)90239-X.
Bass BL: RNA editing by adenosine deaminases that act on RNA. Annu Rev Biochem. 2002, 71: 817-846. 10.1146/annurev.biochem.71.110601.135501.
Nishikura K: Functions and regulation of RNA editing by ADAR deaminases. Annu Rev Biochem. 2010, 79: 321-349. 10.1146/annurev-biochem-060208-105251.
Chen CX, Cho DS, Wang Q, Lai F, Carter KC, Nishikura K: A third member of the RNA-specific adenosine deaminase gene family, ADAR3, contains both single- and double-stranded RNA binding domains. RNA. 2000, 6: 755-767. 10.1017/S1355838200000170.
Tonkin LA, Saccomanno L, Morse DP, Brodigan T, Krause M, Bass BL: RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans. EMBO J. 2002, 21: 6025-6035. 10.1093/emboj/cdf607.
Palladino MJ, Keegan LP, O'Connell MA, Reenan RA: dADAR, a Drosophila double-stranded RNA-specific adenosine deaminase is highly developmentally regulated and is itself a target for RNA editing. RNA. 2000, 6: 1004-1018. 10.1017/S1355838200000248.
Palavicini JP, O'Connell MA, Rosenthal JJ: An extra double-stranded RNA binding domain confers high activity to a squid RNA editing enzyme. RNA. 2009, 15: 1208-1218. 10.1261/rna.1471209.
Jin Y, Zhang W, Li Q: Origins and evolution of ADAR-mediated RNA editing. IUBMB Life. 2009, 61: 572-578. 10.1002/iub.207.
Wolf J, Gerber AP, Keller W: tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli. EMBO J. 2002, 21: 3841-3851. 10.1093/emboj/cdf362.
Gerber AP, Keller W: RNA editing by base deamination: more enzymes, more targets, new mysteries. Trends Biochem Sci. 2001, 26: 376-384. 10.1016/S0968-0004(01)01827-8.
Palavicini JP, Correa-Rojas RA, Rosenthal JJ: Extra double-stranded RNA binding domain (dsRBD) in a squid RNA editing enzyme confers resistance to high salt environment. J Biol Chem. 2012, 287: 17754-17764. 10.1074/jbc.M112.366005.
Garrett S, Rosenthal JJ: RNA editing underlies temperature adaptation in K+ channels from polar octopuses. Science. 2012, 335: 848-851. 10.1126/science.1212795.
Savva YA, Jepson JE, Sahin A, Sugden AU, Dorsky JS, Alpert L, Lawrence C, Reenan RA: Auto-regulatory RNA editing fine-tunes mRNA re-coding and complex behaviour in Drosophila. Nat Commun. 2012, 3: 790-
Rich A, Zhang S: Timeline: Z-DNA: the long road to biological function. Nat Rev Genet. 2003, 4: 566-572.
Schwartz T, Rould MA, Lowenhaupt K, Herbert A, Rich A: Crystal structure of the Zalpha domain of the human editing enzyme ADAR1 bound to left-handed Z-DNA. Science. 1999, 284: 1841-1845. 10.1126/science.284.5421.1841.
Herbert A, Alfken J, Kim YG, Mian IS, Nishikura K, Rich A: A Z-DNA binding domain present in the human editing enzyme, double-stranded RNA adenosine deaminase. Proc Nat Acad Sci USA. 1997, 94: 8421-8426. 10.1073/pnas.94.16.8421.
Berger I, Winston W, Manoharan R, Schwartz T, Alfken J, Kim YG, Lowenhaupt K, Herbert A, Rich A: Spectroscopic characterization of a DNA-binding domain, Z alpha, from the editing enzyme, dsRNA adenosine deaminase: evidence for left-handed Z-DNA in the Z alpha-DNA complex. Biochemistry. 1998, 37: 13313-13321. 10.1021/bi9813126.
Oh DB, Kim YG, Rich A: Z-DNA-binding proteins can act as potent effectors of gene expression in vivo. Proc Nat Acad Sci USA. 2002, 99: 16666-16671. 10.1073/pnas.262672699.
Macbeth MR, Schubert HL, Vandemark AP, Lingam AT, Hill CP, Bass BL: Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing. Science. 2005, 309: 1534-1539. 10.1126/science.1113150.
Melcher T, Maas S, Herb A, Sprengel R, Higuchi M, Seeburg PH: RED2, a brain-specific member of the RNA-specific adenosine deaminase family. J Biol Chem. 1996, 271: 31795-31798. 10.1074/jbc.271.50.31795.
Melcher T, Maas S, Herb A, Sprengel R, Seeburg PH, Higuchi M: A mammalian RNA editing enzyme. Nature. 1996, 379: 460-464. 10.1038/379460a0.
Patterson JB, Samuel CE: Expression and regulation by interferon of a double-stranded-RNA-specific adenosine deaminase from human cells: evidence for two forms of the deaminase. Mol Cell Biol. 1995, 15: 5376-5388.
Desterro JM, Keegan LP, Lafarga M, Berciano MT, O'Connell M, Carmo-Fonseca M: Dynamic association of RNA-editing enzymes with the nucleolus. J Cell Sci. 2003, 116: 1805-1818. 10.1242/jcs.00371.
Sansam CL, Wells KS, Emeson RB: Modulation of RNA editing by functional nucleolar sequestration of ADAR2. Proc Nat Acad Sci USA. 2003, 100: 14018-14023. 10.1073/pnas.2336131100.
Poulsen H, Nilsson J, Damgaard CK, Egebjerg J, Kjems J: CRM1 mediates the export of ADAR1 through a nuclear export signal within the Z-DNA binding domain. Mol Cell Biol. 2001, 21: 7862-7871. 10.1128/MCB.21.22.7862-7871.2001.
Jepson JE, Savva YA, Yokose C, Sugden AU, Sahin A, Reenan RA: Engineered alterations in RNA editing modulate complex behavior in Drosophila: regulatory diversity of adenosine deaminase acting on RNA (ADAR) targets. J Biol Chem. 2011, 286: 8325-8337. 10.1074/jbc.M110.186817.
Hoopengardner B, Bhalla T, Staber C, Reenan R: Nervous system targets of RNA editing identified by comparative genomics. Science. 2003, 301: 832-836. 10.1126/science.1086763.
Wulff BE, Sakurai M, Nishikura K: Elucidating the inosinome: global approaches to adenosine-to-inosine RNA editing. Nat Rev Genet. 2011, 12: 81-85. 10.1038/nrg2915.
Graveley BR, Brooks AN, Carlson JW, Duff MO, Landolin JM, Yang L, Artieri CG, van Baren MJ, Boley N, Booth BW, Brown JB, Cherbas L, Davis CA, Dobin A, Li R, Lin W, Malone JH, Mattiuzzo NR, Miller D, Sturgill D, Tuch BB, Zaleski C, Zhang D, Blanchette M, Dudoit S, Eads B, Green RE, Hammonds A, Jiang L, Kapranov P, et al: The developmental transcriptome of Drosophila melanogaster. Nature. 2011, 417: 483-479.
Li JB, Levanon EY, Yoon JK, Aach J, Xie B, Leproust E, Zhang K, Gao Y, Church GM: Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing. Science. 2009, 324: 1210-1213. 10.1126/science.1170995.
Nishikura K: Editor meets silencer: crosstalk between RNA editing and RNA interference. Nat Rev. 2006, 7: 919-931. 10.1038/nrm2061.
Bhalla T, Rosenthal JJ, Holmgren M, Reenan R: Control of human potassium channel inactivation by editing of a small mRNA hairpin. Nat Struct Mol Biol. 2004, 11: 950-956. 10.1038/nsmb825.
Hanrahan CJ, Palladino MJ, Ganetzky B, Reenan RA: RNA editing of the Drosophila para Na(+) channel transcript. Evolutionary conservation and developmental regulation. Genetics. 2000, 155: 1149-1160.
Keegan LP, Brindle J, Gallo A, Leroy A, Reenan RA, O'Connell MA: Tuning of RNA editing by ADAR is required in Drosophila. EMBO J. 2005, 24: 2183-2193. 10.1038/sj.emboj.7600691.
Ohlson J, Pedersen JS, Haussler D, Ohman M: Editing modifies the GABA(A) receptor subunit alpha3. RNA. 2007, 13: 698-703. 10.1261/rna.349107.
Burns CM, Chu H, Rueter SM, Hutchinson LK, Canton H, Sanders-Bush E, Emeson RB: Regulation of serotonin-2C receptor G-protein coupling by RNA editing. Nature. 1997, 387: 303-308. 10.1038/387303a0.
Higuchi M, Single FN, Kohler M, Sommer B, Sprengel R, Seeburg PH: RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiency. Cell. 1993, 75: 1361-1370. 10.1016/0092-8674(93)90622-W.
Lomeli H, Mosbacher J, Melcher T, Hoger T, Geiger JR, Kuner T, Monyer H, Higuchi M, Bach A, Seeburg PH: Control of kinetic properties of AMPA receptor channels by nuclear RNA editing. Science. 1994, 266: 1709-1713. 10.1126/science.7992055.
Wang Q, O'Brien PJ, Chen CX, Cho DS, Murray JM, Nishikura K: Altered G protein-coupling functions of RNA editing isoform and splicing variant serotonin2C receptors. J Neurochem. 2000, 74: 1290-1300. 10.1046/j.1471-4159.2000.741290.x.
Reenan RA: Molecular determinants and guided evolution of species-specific RNA editing. Nature. 2005, 434: 409-413. 10.1038/nature03364.
Lehmann KA, Bass BL: Double-stranded RNA adenosine deaminases ADAR1 and ADAR2 have overlapping specificities. Biochemistry. 2000, 39: 12875-12884. 10.1021/bi001383g.
Polson AG, Bass BL: Preferential selection of adenosines for modification by double-stranded RNA adenosine deaminase. EMBO. 1994, 13: 5701-5711.
Wong SK, Sato S, Lazinski DW: Substrate recognition by ADAR1 and ADAR2. RNA. 2001, 7: 846-858. 10.1017/S135583820101007X.
Rieder LE, Reenan RA: The intricate relationship between RNA structure, editing, and splicing. Sem Cell Dev Biol. 2011, 23: 281-288.
Dingledine R, Borges K, Bowie D, Traynelis SF: The glutamate receptor ion channels. Pharmacol Rev. 1999, 51: 7-61.
Ingleby L, Maloney R, Jepson J, Horn R, Reenan R: Regulated RNA editing and functional epistasis in Shaker potassium channels. J Gen Physiol. 2009, 133: 17-27. 10.1085/jgp.200810133.
Rosenthal JJ, Bezanilla F: Extensive editing of mRNAs for the squid delayed rectifier K+ channel regulates subunit tetramerization. Neuron. 2002, 34: 743-757. 10.1016/S0896-6273(02)00701-8.
Seeburg PH, Single F, Kuner T, Higuchi M, Sprengel R: Genetic manipulation of key determinants of ion flow in glutamate receptor channels in the mouse. Brain Res. 2001, 907: 233-243. 10.1016/S0006-8993(01)02445-3.
Reenan RA, Hanrahan CJ, Ganetzky B: The mle(napts) RNA helicase mutation in drosophila results in a splicing catastrophe of the para Na+ channel transcript in a region of RNA editing. Neuron. 2000, 25: 139-149. 10.1016/S0896-6273(00)80878-8.
Valente L, Nishikura K: ADAR gene family and A-to-I RNA editing: diverse roles in posttranscriptional gene regulation. Prog Nucleic Acid Res Mol Biol. 2005, 79: 299-338.
Rueter SM, Dawson TR, Emeson RB: Regulation of alternative splicing by RNA editing. Nature. 1999, 399: 75-80. 10.1038/19992.
Feng Y, Sansam CL, Singh M, Emeson RB: Altered RNA editing in mice lacking ADAR2 autoregulation. Mol Cell Biol. 2006, 26: 480-488. 10.1128/MCB.26.2.480-488.2006.
Schoft VK, Schopoff S, Jantsch MF: Regulation of glutamate receptor B pre-mRNA splicing by RNA editing. Nucleic Acids Res. 2007, 35: 3723-3732. 10.1093/nar/gkm314.
Bernstein E, Caudy AA, Hammond SM, Hannon GJ: Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature. 2001, 409: 363-366. 10.1038/35053110.
Hannon GJ: RNA interference. Nature. 2002, 418: 244-251. 10.1038/418244a.
Scadden AD, Smith CW: RNAi is antagonized by A→I hyper-editing. EMBO Rep. 2001, 2: 1107-1111. 10.1093/embo-reports/kve244.
Scadden AD, Smith CW: Specific cleavage of hyper-edited dsRNAs. EMBO J. 2001, 20: 4243-4252. 10.1093/emboj/20.15.4243.
Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004, 116: 281-297. 10.1016/S0092-8674(04)00045-5.
Meister G, Landthaler M, Dorsett Y, Tuschl T: Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. RNA. 2004, 10: 544-550. 10.1261/rna.5235104.
Blow MJ, Grocock RJ, van Dongen S, Enright AJ, Dicks E, Futreal PA, Wooster R, Stratton MR: RNA editing of human microRNAs. Genome Biol. 2006, 7: R27-10.1186/gb-2006-7-4-r27.
Luciano DJ, Mirsky H, Vendetti NJ, Maas S: RNA editing of a miRNA precursor. RNA. 2004, 10: 1174-1177. 10.1261/rna.7350304.
Yang W, Chendrimada TP, Wang Q, Higuchi M, Seeburg PH, Shiekhattar R, Nishikura K: Modulation of microRNA processing and expression through RNA editing by ADAR deaminases. Nat Struct Mol Biol. 2006, 13: 13-21. 10.1038/nsmb1041.
Kawahara Y, Zinshteyn B, Sethupathy P, Iizasa H, Hatzigeorgiou AG, Nishikura K: Redirection of silencing targets by adenosine-to-inosine editing of miRNAs. Science. 2007, 315: 1137-1140. 10.1126/science.1138050.
Gu T, Buaas FW, Simons AK, Ackert-Bicknell CL, Braun RE, Hibbs MA: Canonical A-to-I and C-to-U RNA editing is enriched at 3'UTRs and microRNA target sites in multiple mouse tissues. PLoS One. 2012, 7: e33720-10.1371/journal.pone.0033720.
Athanasiadis A, Rich A, Maas S: Widespread A-to-I RNA editing of Alu-containing mRNAs in the human transcriptome. PLoS Biol. 2004, 2: e391-10.1371/journal.pbio.0020391.
Kim DD, Kim TT, Walsh T, Kobayashi Y, Matise TC, Buyske S, Gabriel A: Widespread RNA editing of embedded alu elements in the human transcriptome. Genome Res. 2004, 14: 1719-1725. 10.1101/gr.2855504.
Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, Funke R, Gage D, Harris K, Heaford A, Howland J, Kann L, Lehoczky J, LeVine R, McEwan P, McKernan K, Meldrim J, Mesirov JP, Miranda C, Morris W, Naylor J, Raymond C, Rosetti M, Santos R, Sheridan A, Sougnez C, et al: Initial sequencing and analysis of the human genome. Nature. 2001, 409: 860-921. 10.1038/35057062.
Paz-Yaacov N, Levanon EY, Nevo E, Kinar Y, Harmelin A, Jacob-Hirsch J, Amariglio N, Eisenberg E, Rechavi G: Adenosine-to-inosine RNA editing shapes transcriptome diversity in primates. Proc Nat Acad Sci USA. 2010, 107: 12174-12179. 10.1073/pnas.1006183107.
Lev-Maor G, Sorek R, Levanon EY, Paz N, Eisenberg E, Ast G: RNA-editing-mediated exon evolution. Genome Biol. 2007, 8: R29-10.1186/gb-2007-8-2-r29.
Sakurai M, Yano T, Kawabata H, Ueda H, Suzuki T: Inosine cyanoethylation identifies A-to-I RNA editing sites in the human transcriptome. Nat Chem Biol. 6: 733-740.
Valente L, Nishikura K: RNA binding-independent dimerization of adenosine deaminases acting on RNA and dominant negative effects of nonfunctional subunits on dimer functions. J Biol Chem. 2007, 282: 16054-16061. 10.1074/jbc.M611392200.
Bycroft M, Grunert S, Murzin AG, Proctor M, St Johnston D: NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5. EMBO J. 1995, 14: 3563-3571.
Nanduri S, Carpick BW, Yang Y, Williams BR, Qin J: Structure of the double-stranded RNA-binding domain of the protein kinase PKR reveals the molecular basis of its dsRNA-mediated activation. EMBO J. 1998, 17: 5458-5465. 10.1093/emboj/17.18.5458.
Ryter JM, Schultz SC: Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA. EMBO J. 1998, 17: 7505-7513. 10.1093/emboj/17.24.7505.
Draper DE: Protein-RNA recognition. Annu Rev Biochem. 1995, 64: 593-620. 10.1146/annurev.bi.64.070195.003113.
Nishikura K, Yoo C, Kim U, Murray JM, Estes PA, Cash FE, Liebhaber SA: Substrate specificity of the dsRNA unwinding/modifying activity. EMBO J. 1991, 10: 3523-3532.
Eggington JM, Greene T, Bass BL: Predicting sites of ADAR editing in double-stranded RNA. Nat Commun. 2011, 2: 319-
Stephens OM, Haudenschild BL, Beal PA: The binding selectivity of ADAR2's dsRBMs contributes to RNA-editing selectivity. Chem Biol. 2004, 11: 1239-1250. 10.1016/j.chembiol.2004.06.009.
Maas S, Kawahara Y, Tamburro KM, Nishikura K: A-to-I RNA editing and human disease. RNA Biol. 2006, 3: 1-9. 10.4161/rna.3.1.2495.
Miyamura Y, Suzuki T, Kono M, Inagaki K, Ito S, Suzuki N, Tomita Y: Mutations of the RNA-specific adenosine deaminase gene (DSRAD) are involved in dyschromatosis symmetrica hereditaria. Am J Hum Genet. 2003, 73: 693-699. 10.1086/378209.
Rice GI, Kasher PR, Forte GM, Mannion NM, Greenwood SM, Szynkiewicz M, Dickerson JE, Bhaskar SS, Zampini M, Briggs TA, Jenkinson EM, Bacino CA, Battini R, Bertini E, Brogan PA, Brueton LA, Carpanelli M, De Laet C, de Lonlay P, Del Toro M, Desguerre I, Fazzi E, Garcia-Cazorla A, Heiberg A, Kawaguchi M, Kumar R, Lin JP, Lourenco CM, Male AM, Marques W, et al: Mutations in ADAR1 cause Aicardi-Goutieres syndrome associated with a type I interferon signature. Nat Genet. 2012, 44: 1243-1248. 10.1038/ng.2414.
Cattaneo R: Biased (A→I) hypermutation of animal RNA virus genomes. Curr Opin Genet Dev. 1994, 4: 895-900. 10.1016/0959-437X(94)90076-0.
Fischer SE, Butler MD, Pan Q, Ruvkun G: Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7. Nature. 2008, 455: 491-496. 10.1038/nature07274.
Peters NT, Rohrbach JA, Zalewski BA, Byrkett CM, Vaughn JC: RNA editing and regulation of Drosophila 4f-rnp expression by sas-10 antisense readthrough mRNA transcripts. RNA. 2003, 9: 698-710. 10.1261/rna.2120703.
Wu D, Lamm AT, Fire AZ: Competition between ADAR and RNAi pathways for an extensive class of RNA targets. Nat Struct Mol Biol. 2011, 18: 1094-1101. 10.1038/nsmb.2129.
Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, Okada TN, Siomi MC, Siomi H: Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells. Nature. 2008, 453: 793-797. 10.1038/nature06938.
Chung WJ, Okamura K, Martin R, Lai EC: Endogenous RNA interference provides a somatic defense against Drosophila transposons. Curr Biol. 2008, 18: 795-802. 10.1016/j.cub.2008.05.006.
Czech B, Malone CD, Zhou R, Stark A, Schlingeheyde C, Dus M, Perrimon N, Kellis M, Wohlschlegel JA, Sachidanandam R, Hannon GJ, Brennecke J: An endogenous small interfering RNA pathway in Drosophila. Nature. 2008, 453: 798-802. 10.1038/nature07007.
Ghildiyal M, Seitz H, Horwich MD, Li C, Du T, Lee S, Xu J, Kittler EL, Zapp ML, Weng Z, Zamore PD: Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. Science. 2008, 320: 1077-1081. 10.1126/science.1157396.
Fagegaltier D, Bouge AL, Berry B, Poisot E, Sismeiro O, Coppee JY, Theodore L, Voinnet O, Antoniewski C: The endogenous siRNA pathway is involved in heterochromatin formation in Drosophila. Proc Nat Acad Sci USA. 2009, 106: 21258-21263. 10.1073/pnas.0809208105.
Yang Y, Lv J, Gui B, Yin H, Wu X, Zhang Y, Jin Y: A-to-I RNA editing alters less-conserved residues of highly conserved coding regions: implications for dual functions in evolution. RNA. 2008, 14: 1516-1525. 10.1261/rna.1063708.
Tian N, Wu X, Zhang Y, Jin Y: A-to-I editing sites are a genomically encoded G: implications for the evolutionary significance and identification of novel editing sites. RNA. 2008, 14: 211-216.
Gonzalez C, Lopez-Rodriguez A, Srikumar D, Rosenthal JJ, Holmgren M: Editing of human K(V)1.1 channel mRNAs disrupts binding of the N-terminus tip at the intracellular cavity. Nat Commun. 2011, 2: 436-
Huang H, Tan BZ, Shen Y, Tao J, Jiang F, Sung YY, Ng CK, Raida M, Köhr G, Higuchi M, Fatemi-Shariatpanahi H, Harden B, Yue DT, Soong TW: RNA editing of the IQ domain in Ca(v)1.3 channels modulates their Ca2+-dependent inactivation. Neuron. 2012, 73: 304-316. 10.1016/j.neuron.2011.11.022.
Ryan MY, Maloney R, Fineberg JD, Reenan RA, Horn R: RNA editing in eag potassium channels: Biophysical consequences of editing a conserved S6 residue. Channels (Austin). 2012, 6: 443-452. 10.4161/chan.22314.
Jepson JE, Savva YA, Jay KA, Reenan RA: Visualizing adenosine-to-inosine RNA editing in the Drosophila nervous system. Nat Methods. 2012, 9: 189-194.
Houweling AR, Brecht M: Behavioural report of single neuron stimulation in somatosensory cortex. Nature. 2008, 451: 65-68. 10.1038/nature06447.
Li CY, Poo MM, Dan Y: Burst spiking of a single cortical neuron modifies global brain state. Science. 2009, 324: 643-646. 10.1126/science.1169957.
The authors thank James EC Jepson for image contributions.
The authors declare that they have no competing interests.
Yiannis A Savva, Leila E Rieder contributed equally to this work.
About this article
Cite this article
Savva, Y.A., Rieder, L.E. & Reenan, R.A. The ADAR protein family. Genome Biol 13, 252 (2012). https://doi.org/10.1186/gb-2012-13-12-252
- dsRNA binding proteins
- post-transcriptional modification
- RNA editing
- RNA splicing