Visualization, mapping and sequencing of megabase lengths of DNA
© Mir et al; licensee BioMed Central Ltd. 2010
Published: 11 October 2010
Prior to replication, a chromosome comprises a single length of DNA. We report on new methods for handling tens of kilobase to megabase lengths of single DNA molecules. We further report on the direct visualization of sequence organisation and the action of processive enzymatic activity along the molecules. We show how individual molecules can be captured and processed on single molecule microarrays  using a ligation base sequencing biochemistry that we have developed . Finally, we describe how our methods will complement next generation DNA sequencing.
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