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Open Access

Visualization, mapping and sequencing of megabase lengths of DNA

  • Kalim Mir1,
  • Liqin Dong1,
  • David Bauer1 and
  • Giuseppe Scozzafava1
Genome Biology201011(Suppl 1):P28

https://doi.org/10.1186/gb-2010-11-s1-p28

Published: 11 October 2010

Prior to replication, a chromosome comprises a single length of DNA. We report on new methods for handling tens of kilobase to megabase lengths of single DNA molecules. We further report on the direct visualization of sequence organisation and the action of processive enzymatic activity along the molecules. We show how individual molecules can be captured and processed on single molecule microarrays [1] using a ligation base sequencing biochemistry that we have developed [2]. Finally, we describe how our methods will complement next generation DNA sequencing.

Authors’ Affiliations

(1)
The Wellcome Trust Centre for Human Genetics, University of Oxford

References

  1. Mir K: Ultrasensitive RNA profiling: counting single molecules on microarrays. Genome. Res. 2006, 16: 1195-7. 10.1101/gr.5825506. Epub 2006PubMedView ArticleGoogle Scholar
  2. Mir , et al: Sequencing by Cyclic Ligation and Cleavage (CycLiC) directly on a microarray captured template. Nucleic Acids Rese. 2009, 37: e5-10.1093/nar/gkn906.View ArticleGoogle Scholar

Copyright

© Mir et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

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