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Visualization, mapping and sequencing of megabase lengths of DNA
Genome Biology volume 11, Article number: P28 (2010)
Prior to replication, a chromosome comprises a single length of DNA. We report on new methods for handling tens of kilobase to megabase lengths of single DNA molecules. We further report on the direct visualization of sequence organisation and the action of processive enzymatic activity along the molecules. We show how individual molecules can be captured and processed on single molecule microarrays  using a ligation base sequencing biochemistry that we have developed . Finally, we describe how our methods will complement next generation DNA sequencing.
Mir K: Ultrasensitive RNA profiling: counting single molecules on microarrays. Genome. Res. 2006, 16: 1195-7. 10.1101/gr.5825506. Epub 2006
Mir , et al: Sequencing by Cyclic Ligation and Cleavage (CycLiC) directly on a microarray captured template. Nucleic Acids Rese. 2009, 37: e5-10.1093/nar/gkn906.
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Mir, K., Dong, L., Bauer, D. et al. Visualization, mapping and sequencing of megabase lengths of DNA. Genome Biol 11, P28 (2010). https://doi.org/10.1186/gb-2010-11-s1-p28
- Enzymatic Activity
- Single Molecule
- Direct Visualization
- Base Sequencing
- Individual Molecule