- Poster presentation
- Open Access
Visualization, mapping and sequencing of megabase lengths of DNA
© Mir et al; licensee BioMed Central Ltd. 2010
- Published: 11 October 2010
- Enzymatic Activity
- Single Molecule
- Direct Visualization
- Base Sequencing
- Individual Molecule
Prior to replication, a chromosome comprises a single length of DNA. We report on new methods for handling tens of kilobase to megabase lengths of single DNA molecules. We further report on the direct visualization of sequence organisation and the action of processive enzymatic activity along the molecules. We show how individual molecules can be captured and processed on single molecule microarrays  using a ligation base sequencing biochemistry that we have developed . Finally, we describe how our methods will complement next generation DNA sequencing.
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This article is published under license to BioMed Central Ltd.