Structural and functional map of a bacterial nucleoid
© BioMed Central Ltd 2009
Published: 11 December 2009
Genome-wide mapping of transcription factor-DNA interactions in bacterial chromosomes in vivo has begun to reveal global zones occupied by these factors that serve two purposes: compacting the bacterial DNA and influencing global programs of gene transcription.
In single-celled organisms such as bacteria economy is critical, including the efficient use of space in the tiny cell. Although gene density in bacterial genomes is high, the chromosomes are still long macromolecules that must be compacted by at least three orders of magnitude to fit into the space available [1, 2], and the mechanism of chromosomal packing in bacteria and the proteins involved is a long-standing question. In a recent study published in Molecular Cell, Saeed Tavoizie and colleagues (Vora et al. ) have investigated protein binding across the complete Escherichia coli genome and have revealed extended regions of high protein occupancy. Together with other recent studies, this work provides valuable information on the chromosomal organization by DNA-binding proteins in bacteria and will aid understanding of their large-scale effects on gene expression.
Chromosomal size and dynamics in bacteria
A bacterial genome typically comprises a single circular DNA molecule, usually between 1.5 and 10 Mbp in free-living bacteria [4, 5], which in vivo is packaged with proteins into a distinct structure known as the bacterial nucleoid. The information encoded in one bacterial genome directs all functions necessary to maintain a functional and self-replicating living system, from basic tasks such as nutrient and energy uptake to complex coordinated ones, such as cell division. Initial observations indicated that when DNA is released from lyzed bacteria, the space it occupies is four to ten times larger than the cell itself, even though the DNA preserves supercoiled loops . This implied that chromosomes are even more compacted inside the cell, probably by auxiliary proteins [2, 7]. In addition to DNA gyrase and DNA topoisomerase I, which maintain supercoiling levels of DNA [6, 8], the so-called nucleoid-associated proteins (NAPs) were proposed to be in charge of most chromosomal remodeling tasks. Among others, Ishihama and colleagues have studied NAPs extensively, and at the end of the 1990s, Ali Azam et al. [9, 10] found that in cultured cells, each NAP is maximally expressed during specific growth phases.
The regulatory regions of transcription units are located in noncoding DNA sequences where transcription factors and RNA polymerases bind to the DNA to initiate transcription. The bacterial nucleoid structure is natively able to permit transcription, despite the microscopically observed loops and predicted further levels of genome compaction. This is probably due to the fact that the level of compaction is not as restrictive as that of eukaryotic chromatin .
Even when transcription is permitted in bacteria, the effects of chromosomal compaction on gene expression are still not clear. Because nucleoid organization can be described on both a physical and a functional basis, these two properties should be analyzed and understood together. Nucleoid topology is strongly related to the binding patterns of NAPs. All the major NAPs, with the exception of Dps (the DNA-binding protein in starved cells), have been found experimentally to have a functional association with the regulation of gene expression. These regulatory NAPs are: Fis (factor for inversion stimulation), HU (histone-like protein), H-NS (histone-like nucleoid structuring protein), and IHF (integration host factor). The concentrations of these proteins vary in different growth phases, from 10,000 to 60,000 monomers per cell, in contrast to local regulators such as LacI, which is present at a maximum of 20 monomers per cell . These observations, together with knowledge of the hierarchy of regulatory networks, have led to the hypothesis of 'analog' and 'digital' components of gene regulation in bacteria. The analog component is represented by the wide influence of superhelical and chromosomal loops (mediated by NAPs) in background regulation, and the digital component by the qualitatively more effective (almost binary) regulation exerted by DNA-binding specific transcription factors [13, 14].
Genome-wide chromosomal occupancy by DNA-binding proteins
In their recent study Vora et al.  aimed at obtaining all the protein-DNA complexes present in E. coli at early and late exponential growth phases, respectively. This genome-wide screening methodology is known as in vivo protein occupancy display (IPOD). To recover occupied DNA sequences at a high resolution, they obtained short fragments (50 bp) of DNA protected by proteins and then used a high-density tiling array to analyze the DNA. In order to cover the entire E. coli genome, the array was composed of overlapping oligomers of 25 bp, designed to locate a DNA fragment at a resolution of 4 bp of genomic DNA.
Vora et al.  detected 2,063 individual protein-occupied sites, some of which were found in close proximity to each other - forming what the authors call extended protein occupancy domains (EPODs) with lengths ranging from 1 to 14 kbp (Figure 1). They then determined the transcriptional profiles of the EPODs by DNA microarray analysis and found that they fell into two groups - highly expressed (heEPODs) and transcriptionally silent (tsEPODs). Using previous data of Grainger et al. , who had determined DNA polymerase occupancy in the same growing conditions, Vora et al. found that the 121 heEPODs showed high polymerase occupancy whereas the 151 tsEPODs showed lower occupancy. The 121 highly occupied zones included highly expressed genes such as those for ribosomal proteins, while the 151 tsEPODs had a high content of predicted or hypothetical open reading frames that, interestingly, corresponded to transcriptionally silent genes (Figure 1). An extensive search for putative H-NS-, Fis- and IHF-binding sites (available from RegulonDB ) in the EPOD sequences indicated that binding sites for these proteins are overrepresented in tsEPODs, whereas only Fis showed overrepresentation of binding sites within heEPODs. This was as expected, as Fis is maximally expressed at the beginning of the exponential growth phase and regulates the transcription of the ribosomal genes, among others. On this basis, Vora et al.  hypothesize that tsEPODs may comprise the predicted structural organizational center of the bacterial nucleoid, potentially also carrying out the important functional task of repression of silent DNA sequences by H-NS [21, 22].
Taking it further
The work of Tavazoie and colleagues  opens up the possibility of studying, at a high resolution, the zones of the nucleoid occupied by the entire repertoire of transcription factors. The next step should be to obtain chromosomal occupancy profiles at different growing phases - that is, lag, early, mid, and late exponential and early and late stationary phases. With these data, investigators should be able to obtain a dynamic picture of protein occupancy for NAPs along the different growth phases of a bacterial culture. As each NAP is produced maximally at different growth phases, one would expect that the nucleoid dynamics would be different, influencing the running of global transcriptional programs within each growth phase - that is, the analog programs [13, 14]. In parallel, computational efforts should be made to find all putative binding sites in DNA for the approximately 81 transcription factors in RegulonDB  that currently have experimentally annotated binding sites. This will enable determination of the digital control exerted in each growth phase. To reveal the complete picture of the dynamic nucleoid, efforts should be made to characterize the binding sites for the complete repertoire of around 300 transcription factors in the E. coli genome. It is intriguing that chromosomal loops, EPODs and the maximal operon size are all around 10 kbp. If not a coincidence, this could reflect the presence in E. coli of local supercoiling domains whose boundaries limit coordinated transcription, by analogy with observations in eukaryotes .
The authors are grateful for the comments of colleagues and reviewers which helped improve the article. AM-R was supported during her PhD studies (Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México) by a fellowship from the Consejo Nacional de Ciencia y Tecnología (Mexico). This work was partially supported by the "Consejos de Ciencia y Tecnología Nacional (102854) y del Estado de Guanajuato" (Young Researcher grants) given to AM-A and CONACYT (103686) and NIH grant number GM071962-06 given to JC-V.
- Krawiec S, Riley M: Organization of the bacterial chromosome. Microbiol Rev. 1990, 54: 502-539.PubMedPubMed CentralGoogle Scholar
- Kellenberger E: Functional consequences of improved structural information on bacterial nucleoids. Res Microbiol. 1991, 142: 229-238. 10.1016/0923-2508(91)90035-9.PubMedView ArticleGoogle Scholar
- Vora T, Hottes AK, Tavazoie S: Protein occupancy landscape of a bacterial genome. Mol Cell. 2009, 35: 247-253. 10.1016/j.molcel.2009.06.035.PubMedPubMed CentralView ArticleGoogle Scholar
- Perez-Rueda E, Janga SC, Martinez-Antonio A: Scaling relationship in the gene content of transcriptional machinery in bacteria. Mol Biosyst. 2009, 5: 1494-1501. 10.1039/b907384a.PubMedView ArticleGoogle Scholar
- Ochman H, Moran NA: Genes lost and genes found: evolution of bacterial pathogenesis and symbiosis. Science. 2001, 292: 1096-1099. 10.1126/science.1058543.PubMedView ArticleGoogle Scholar
- Postow L, Hardy CD, Arsuaga J, Cozzarelli NR: Topological domain structure of the Escherichia coli chromosome. Genes Dev. 2004, 18: 1766-1779. 10.1101/gad.1207504.PubMedPubMed CentralView ArticleGoogle Scholar
- Woldringh CL, Jensen PR, Westerhoff HV: Structure and partitioning of bacterial DNA: determined by a balance of compaction and expansion forces?. FEMS Microbiol Lett. 1995, 131: 235-242. 10.1111/j.1574-6968.1995.tb07782.x.PubMedView ArticleGoogle Scholar
- Travers A, Muskhelishvili G: DNA supercoiling - a global transcriptional regulator for enterobacterial growth?. Nat Rev Microbiol. 2005, 3: 157-169. 10.1038/nrmicro1088.PubMedView ArticleGoogle Scholar
- Ali Azam T, Iwata A, Nishimura A, Ueda S, Ishihama A: Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid. J Bacteriol. 1999, 181: 6361-6370.PubMedPubMed CentralGoogle Scholar
- Azam TA, Ishihama A: Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999, 274: 33105-33113. 10.1074/jbc.274.46.33105.PubMedView ArticleGoogle Scholar
- Struhl K: Fundamentally different logic of gene regulation in eukaryotes and prokaryotes. Cell. 1999, 98: 1-4. 10.1016/S0092-8674(00)80599-1.PubMedView ArticleGoogle Scholar
- Elf J, Li GW, Xie XS: Probing transcription factor dynamics at the single-molecule level in a living cell. Science. 2007, 316: 1191-1194. 10.1126/science.1141967.PubMedPubMed CentralView ArticleGoogle Scholar
- Marr C, Geertz M, Hutt MT, Muskhelishvili G: Dissecting the logical types of network control in gene expression profiles. BMC Syst Biol. 2008, 2: 18-10.1186/1752-0509-2-18.PubMedPubMed CentralView ArticleGoogle Scholar
- Janga SC, Salgado H, Martinez-Antonio A: Transcriptional regulation shapes the organization of genes on bacterial chromosomes. Nucleic Acids Res. 2009, 37: 3680-3688. 10.1093/nar/gkp231.PubMedPubMed CentralView ArticleGoogle Scholar
- Ren B, Robert F, Wyrick JJ, Aparicio O, Jennings EG, Simon I, Zeitlinger J, Schreiber J, Hannett N, Kanin E, Volkert TL, Wilson CJ, Bell SP, Young RA: Genome-wide location and function of DNA binding proteins. Science. 2000, 290: 2306-2309. 10.1126/science.290.5500.2306.PubMedView ArticleGoogle Scholar
- Grainger DC, Hurd D, Goldberg MD, Busby SJ: Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome. Nucleic Acids Res. 2006, 34: 4642-4652. 10.1093/nar/gkl542.PubMedPubMed CentralView ArticleGoogle Scholar
- Grainger DC, Hurd D, Harrison M, Holdstock J, Busby SJ: Studies of the distribution of Escherichia coli cAMP-receptor protein and RNA polymerase along the E. coli chromosome. Proc Natl Acad Sci USA. 2005, 102: 17693-17698. 10.1073/pnas.0506687102.PubMedPubMed CentralView ArticleGoogle Scholar
- Cho BK, Barrett CL, Knight EM, Park YS, Palsson BO: Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli. Proc Natl Acad Sci USA. 2008, 105: 19462-19467. 10.1073/pnas.0807227105.PubMedPubMed CentralView ArticleGoogle Scholar
- Wade JT, Reppas NB, Church GM, Struhl K: Genomic analysis of LexA binding reveals the permissive nature of the Escherichia coli genome and identifies unconventional target sites. Genes Dev. 2005, 19: 2619-2630. 10.1101/gad.1355605.PubMedPubMed CentralView ArticleGoogle Scholar
- Gama-Castro S, Jiménez-Jacinto V, Peralta-Gil M, Santos-Zavaleta A, Peñaloza-Spinola MI, Contreras-Moreira B, Segura-Salazar J, Muñiz-Rascado L, Martínez-Flores I, Salgado H, Bonavides-Martínez C, Abreu-Goodger C, Rodríguez-Penagos C, Miranda-Ríos J, Morett E, Merino E, Huerta AM, Treviño-Quintanilla L, Collado-Vides J: RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation. Nucleic Acids Res. 2008, D120-D124. 36 DatabaseGoogle Scholar
- Lang B, Blot N, Bouffartigues E, Buckle M, Geertz M, Gualerzi CO, Mavathur R, Muskhelishvili G, Pon CL, Rimsky S, Stella S, Babu MM, Travers A: High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes. Nucleic Acids Res. 2007, 35: 6330-6337. 10.1093/nar/gkm712.PubMedPubMed CentralView ArticleGoogle Scholar
- Navarre WW, Porwollik S, Wang Y, McClelland M, Rosen H, Libby SJ, Fang FC: Selective silencing of foreign DNA with low GC content by the H-NS protein in Salmonella. Science. 2006, 313: 236-238. 10.1126/science.1128794.PubMedView ArticleGoogle Scholar
- Cremer T, Cremer C: Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat Rev Genet. 2001, 2: 292-301. 10.1038/35066075.PubMedView ArticleGoogle Scholar
- Stothard P, Wishart DS: Circular genome visualization and exploration using CGView. Bioinformatics. 2005, 21: 537-539. 10.1093/bioinformatics/bti054.PubMedView ArticleGoogle Scholar