Figure 5

Expression profiles of uncharacterized sensory histidine kinases that could phosphorylate Spo0A. Gene and operon profiles are ratios compared against the first expressed timepoint. Gray bar indicates the onset of the transitional phase. (a) Activation of Spo0A as represented through the upregulation of the sol operon (black filled diamonds; CAP0162-164) and the production of butanol (green crosses). Activation occurs before spo0A (red filled triangles) reaches peak expression. (b) Expression of the orphan kinases CAC0323 (blue filled diamonds), CAC0437 (green filled triangles), and CAC0903 (red filled circles) relative to the sol operon (black filled diamonds) (right-hand side vertical axis). (c) Expression of the orphan kinases CAC2730 (blue filled squares) and CAC3319 (open red circles) relative to the sol operon (black filled diamonds) (right-hand side vertical axis). (d) Expression of the two-component kinases CAC0225 (green filled circles), CAC0290 (red filled squares), and CAC0863 (open blue diamonds) relative to the sol operon (black filled diamonds) (right-hand side vertical axis). (e) Expression of the two-component kinases CAC1582 (green filled squares), CAC2434 (open blue circles), and CAC3430 (open red diamonds) relative to the sol operon (black filled diamonds) (right-hand side vertical axis). (f) Ranked expression intensities. White denotes a rank of 1, while dark blue denotes a rank of 100 (see scale). Plot covers the entire timecourse, whereas the previous figures only covered the first 14 hours. Gray squares indicate timepoints at which the intensity did not exceed the threshold value.