NC4A2 lines contain a duplication of the same segment of chromosome 2 that is duplicated in Ax3. The duplication appears for the most part identical in all strains derived from Ax3. We show here (a) Kax3(U), (b) NC4A2(Kn), and (c) NC4A2(SC) because they display points of similarity not observed in the other examples of this lineage in our study. The point of inversion of this tandem inverse duplication is to the right of the plot, where some genes (log2 ratios negative) appear to have been deleted in both copies in NC4A2 and KAx3(U). At least one of these genes appears to have been lost in both copies in several other of the Ax3-lineage strains in our study, but unfortunately some of the probes for these genes were not printed well and so our data do not permit us to assess exactly how frequent these deletions are. A segment within the duplication towards the left-hand side appears to be present as a single copy in both NC4A2 lines and in KAx3(U); this runs from DDB0233427 to DDB0191242, and appears to be present in the expected two copies in all other Ax3 derived strains we have studied.