A duplication common to Ax2 lines. (a) All Ax2 strains in our study plus selected other strains of NC4 and non-NC4 backgrounds are displayed. Each block is colored according to the log2 ratio for the comparisons of each strain with reference Ax2(Ka). Since log2 ratios are consistently greater than zero for the duplicated genes in examples of Ax2 other than the reference, we suggest that this region is amplified further in these strains. The genes plotted are: a, DDB0190411; b, DDB0190412; c, DDB0190413; d, DDB0201787 (probe 1); e, DDB0201787 (probe 2); f, DDB0190415; g, DDB0190416; h, DDB0201789; i, DDB0190418; j, DDB0216669; k, DDB0190421; l, DDB0190422; m, DDB0190424; n, DDB0190426; and o, DDB0190427. (b) The breakpoints of the duplication in Ax2(Ka) were confirmed by real-time quantitative PCR, in comparison with Ax4(Ku). Mean log2 ratios ± standard error are shown, summarizing, per gene, four pairwise comparisons of threshold cycles.