- Open Access
Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains
© Hogg et al.; licensee BioMed Central Ltd. 2007
- Received: 9 February 2007
- Accepted: 5 June 2007
- Published: 05 June 2007
The distributed genome hypothesis (DGH) posits that chronic bacterial pathogens utilize polyclonal infection and reassortment of genic characters to ensure persistence in the face of adaptive host defenses. Studies based on random sequencing of multiple strain libraries suggested that free-living bacterial species possess a supragenome that is much larger than the genome of any single bacterium.
We derived high depth genomic coverage of nine nontypeable Haemophilus influenzae (NTHi) clinical isolates, bringing to 13 the number of sequenced NTHi genomes. Clustering identified 2,786 genes, of which 1,461 were common to all strains, with each of the remaining 1,328 found in a subset of strains; the number of clusters ranged from 1,686 to 1,878 per strain. Genic differences of between 96 and 585 were identified per strain pair. Comparisons of each of the NTHi strains with the Rd strain revealed between 107 and 158 insertions and 100 and 213 deletions per genome. The mean insertion and deletion sizes were 1,356 and 1,020 base-pairs, respectively, with mean maximum insertions and deletions of 26,977 and 37,299 base-pairs. This relatively large number of small rearrangements among strains is in keeping with what is known about the transformation mechanisms in this naturally competent pathogen.
A finite supragenome model was developed to explain the distribution of genes among strains. The model predicts that the NTHi supragenome contains between 4,425 and 6,052 genes with most uncertainty regarding the number of rare genes, those that have a frequency of <0.1 among strains; collectively, these results support the DGH.
- Codon Usage
- Horizontal Gene Transfer
- Core Gene
- Clinical Strain
- Otitis Medium With Effusion
Haemophilus influenzae is a Gram-negative bacterium that colonizes the human nasopharynx and is also etiologically associated with a spectrum of acute and chronic diseases. There are six recognized capsular serotypes (a-f), but the majority of clinical strains are unencapsulated and are referred to as nontypeable H. influenzae (NTHi). The type b polysaccharide capsular variants (Hib) are associated with invasive disease, particularly meningitis; however, the introduction of a highly effective vaccine has nearly eliminated this pathogen from developed countries. Recent studies have demonstrated that the NTHi form biofilms on the respiratory mucosa of humans and other mammals and it has been hypothesized that this contributes to the chronicity of these infections [1, 2]. They are the most frequently detected pathogens associated with both the acute and chronic forms of otitis media (OM)  and also are recognized as a seed pathogen in a wide range of chronic polymicrobial infections of the respiratory mucosa, including the cystic fibrosis lung, chronic obstructive pulmonary disease, tracheobronchitis, rhinosinusitis, and mastoiditis [4, 5].
The NTHi are naturally transformable and their genomes demonstrate a high degree of plasticity among strains [4, 6–11]. Previous work from our laboratory has shown that approximately 10% of the genes possessed by each clinically isolated strain are novel with respect to the reference strain Rd KW20 and that the distribution of these genes among the strains is non-uniform . Polyclonal NTHi populations have been associated with chronic disease as well as with nasopharyngeal carriage [4, 12], while other researchers have observed in situ horizontal gene transfer in diseased patients [7, 8, 13]. The twin observations that the NTHi form biofilms during chronic infections and that these infections are often polyclonal suggests that multiple unique strains are co-localized within an environment demonstrated to support greatly elevated rates of horizontal gene transfer [14–18]. These circumstantial evidences suggest that a genetically diverse population may be important to the fitness of H. influenzae as a human pathogen and that continuous horizontal gene transfer among co-colonizing strains is the mechanism that generates the diversity observed in the population. It has been hypothesized that this microbial diversity generation is the counterpoint to the adaptive immune response of the mammalian host . The distributed genome hypothesis (DGH) states that the full complement of genes available to a pathogenic bacterial species exists in a 'supragenome' pool that is not contained by any particular strain, but is available through a genically diverse population of naturally transformable bacterial strains. The distributed genome is not a phenomenon isolated to H. influenzae; comparative genomic studies in other bacterial pathogens, including pneumococcus and Pseudomonas aeruginosa, have demonstrated even greater degrees of genomic plasticity among clinical strains [20, 21]. Moreover, evolutionary studies have demonstrated that pneumococcus uses competence and transformation as a pathogenic mechanism [22–24].
Testing of the DGH and its predictions will provide insight into clinically relevant problems, such as antibiotic resistance, chronic biofilm disease, and serotype-diverse species, which readily adapt to standard vaccinations. Further characterization of the H. influenzae supragenome is a prerequisite to addressing these issues. In this regard we have sequenced the genomes of 11 clinical NTHi isolates, 2 by standard clone-based Sanger sequencing and 9 using the new 454-based pyrosequencing technology. This dataset, combined with the published genomic sequences of Rd and R2866, constitutes the largest set of genomic data collected for H. influenzae to date - the first step towards a characterization of the full complement of genes that collectively define the H. influenzae supragenome. In this paper we present a global comparative analysis that characterizes the distribution of genetic diversity among the strains.
DNA sequence data
Bacterial strains and sources used for whole genome sequencing, comparative genomics, and computation of the NTHi core and supragenomes
NCBI locus tag prefix
Clinical source [reference]
Lab strain, formerly serotype D 
NP isolate from COM patient 
OM isolate, St Louis [10,52]
Blood isolate (meningitis), Seattle [10,53]
AOM isolate, Missouri [54, from A. Ryan]
OME isolate, Pittsburgh 
OME isolate, Pittsburgh 
Otorrhea isolate, Pittsburgh 
OME isolate, Pittsburgh 
Otorrhea isolate, Pittsburgh 
NP isolate 
NP isolate, Michigan *
NP isolate, Michigan *
Sequencing data for the 9 Nthi strains sequenced with 454-technology
H. influenzae strain
40×70 plates sequenced
454 read coverage
No. of Newbler contigs
PCR gap closure?
4 kb clone library?
Final no. of contigs
Determination of gene clustering parameters
Enumeration of gene clusters and genic relationships among NTHi strains
Gene clustering results
Total gene clusters
Core gene clusters
Gene identification and clustering results
H. influenzae strain
Genome size (MB)
No. of AMIgene CDSs found
Total gene clusters
Contingency gene clusters
Unique gene clusters
Whole genome alignments reinforce the great diversity observed among gene clusters
Analysis of inserted and deleted Sequence in 12 strains with respect to Rd KW20
Reference: Rd KW20
Number of insertions
Median insert length (bp)
Mean insert length (bp)
Max insert length (bp)
Total insert length (bp)
Number of deletions
Median deleted length (bp)
Mean deleted length (bp)
Max deleted length (bp)
Total deleted length (bp)
Codon usage analysis
Codon usage comparisons of core, contingency and unique genes
Codon usage comparison of core, contingency and unique genes
Median length (amino acids)
Phage homology analysis
Percentage of genes with probable phage origin per category
Unique genes (1 strain)
Distributed genes (2-12 strains)
Core genes (all strains)
Development of a finite supragenome model
The comparative genomic data presented above are supportive of the DGH and reinforces the concept that, at the species level, there is an H. influenzae supragenome that is much larger than the genome of any single individual strain, and hence many strains must be sequenced to generate an accurate picture of the species supragenome. Among the questions we may ask about the supragenome, the most obvious is, how many strains must be sequenced to observe the entire (or nearly all) of the supragenome?. The problem is similar to determining the read coverage necessary to sequence an entire individual genome using a random shotgun library approach. Lander-Waterman statistics provide an answer in the latter case by using the assumption that reads are independently and randomly sampled from the genome with equal probability. Previously, Tettelin et al.  developed a supragenome model for S. agalactiae that, like Lander-Waterman statistics, is based on the assumption that contingency genes are independently sampled from the supragenome with equal probability, except in the case of rare genes, which are modeled as unique events that appear only once in the entire global population. The model requires four parameters: the number of core genes, the number of contingency genes, the probability of finding a contingency gene, and the expected number of 'unique' genes found per strain. This model predicted that the supragenome of S. agalactiae is infinite in size (that is, the expected number of unique genes found in each strain is non-zero). While the model is an insightful attack on the problem, we question the assumption that contingency genes are sampled in the population with equal probability. It is important to compare the existing model against a new model that does not rely on this assumption.
The Supragenome is represented here by a generative model that emits genomes according to a set of probabilistic rules. The supragenome contains N genes that are modeled as Bernoulli random variables with 'success' probabilities that correspond to the population frequency of each gene. A genome is generated by observing the Bernoulli variables: a gene is present if the corresponding trial is a success and otherwise absent. Each gene variable is assumed to be independent of all other genes. This assumption is sometimes violated in real H. influenzae genomes. For example, genomic islands are sets of genes that are not independent. However, we proceed with this assumption since it significantly reduces the complexity of the model and is reasonable in many cases.
The true population frequencies are, in general, unknown. Therefore, population frequencies are also treated in a probabilistic fashion. It is assumed that there are K discrete classes of genes. Each class k has an associated population frequency, μk. All genes in class k will have population frequency μk. Each of the N genes is assigned to a class according to a probability distribution given by the vector π, where πk is the probability that a gene is assigned to class k. Conceptually, πk is the percentage of genes in the supragenome that have population frequency μk. The assignment of a gene to a class is independent of all other gene assignments.
and requiring that the coefficients are between 0 and 1. The maximization was performed for values of N starting at the minimum possible value (the number of genes actually observed) to 6,000. The combination of N and π that maximized the overall log-likelihood was selected as the best parameter estimate.
Supragenome modeling validation and results
Figure 5 compares model predictions based on 8 strains to actual observations of core genes (shared among the first N strains) and total genes found after sequencing the 9th through 13th strains. In both cases the model predictions follow the observed trends. Figure 6 compares predictions to observations of the number of new genes found in the Nth sequenced strain. Again the model predictions follow the observed trend. Figure 2 compares the best-fit gene distribution (based on 8 strain models) to the observed distribution of genes found in exactly N of 13 strains. Overall, the predicted trends follow the observed distribution; however, the predictions were too low for genes seen in 1 of 13 strains, and too high for genes seen in 2 of 13 strains. This bias may be due to the small sample size (eight strains) used to train the supragenome model. Predictions for genes seen in four to seven strains were also somewhat lower than observed.
The supragenome model predicted an average of 1,776 genes per strain with a standard deviation of 14 genes. Of the 13 strains, the average number of genes was 1,793 with a standard deviation of 62 genes. The model predicted an average of 373 different genes when comparing any two strains with a standard deviation of 17 genes. Among the 13 sequenced strains, the average was 395 with a standard deviation of 91 genes. In both cases the model predication for average was reasonable, while the standard deviation was underestimated by about four-fold. This suggests that the assumptions used for the supragenome model may omit important sources of variation. Genomic islands and other genes that appear together in the genome likely contribute to the total variance.
Maximum likelyhood estimate for size of supragenome and 1/100 likelihood intervals based on 8 and 13 strain training sets
Comparative genomic analyses were performed on 13 H. influenzae strains, 12 clinical isolates and Rd, an acapsular strain derived from a serotype d strain that is not typically associated with disease. The results of these studies demonstrated great genic diversity among the strains on average. This genic diversity is visualized by a dendrogram constructed from the genic differences among strains (Figure 4). A typical pair of strains varied by nearly 400 genes. A phylogeny constructed from MLST housekeeping genes also demonstrates a high degree of allelic diversity. However, the topologies of the MLST and genic trees differ significantly. This indicates that the genic sharing of non-core genes among strains is not always related to the phylogenetic relationships inferred from housekeeping genes. Rd was not an outlier in either tree, suggesting that encapsulated strains share the same supragenome. This reinforces previous research that arrived at the same conclusion using other methods . Cluster analysis revealed nearly 2,800 distinct genes among these 13 strains, while modeling predicts that the species-level supragenome will contain 5,000 or more genes and require the analysis of several hundred strains to be complete. A supragenome containing approximately 5,000 genes would possess nearly three times the number of genes observed in any single strain.
Slightly over half (1,437) of the gene clusters identified are predicted to constitute a necessary set of core genes. The non-core genes in each strain (356 on average) are composed of distributed genes (present in more than one strain, but not all strains) and unique genes that are not represented in any other H. influenzae strains. Genes in the core genome are more likely to display typical H. influenzae codon usage patterns and are rarely homologous to phage-related genes. In contrast, the distributed genes and unique genes are more likely to display atypical codon usage patterns for H. influenzae and are more likely to share homology with phage and other bacterial species, but still the majority of these non-core genes possess codon usage statistics similar to core genes. In fact, out of a total of 736 distributed genes observed among the 13 strains, less than 15% displayed any significant phage homology. Hence, the core genome is wholly specific to H. influenzae, while non-core H. influenzae-specific genes are likely mixed with genes of foreign origin. The subset of contingency genes with typical codon usage patterns and without phage homology will be important candidates for functional studies.
Among the 13 strains examined, 539 unique genes were identified. Our model predicts that most of these 'unique' genes are derived from a pool of approximately 3,000+ low frequency genes. Of these, 25% demonstrate sequence homology to phage genes. The codon usage of these genes is often typical, but more likely than core and distributed genes to diverge from H. influenzae patterns. The origin and importance of the remaining 75% of the unique genes is unclear. Since these genes have not been enriched in the population by positive selection, it is uncertain whether these genes correspond to a functional role in H. influenzae; however, previous studies have demonstrated that 100% of the unique genes examined are expressed as RNA transcripts . It is possible that high levels of horizontal gene transfer between organisms in the H. influenzae environmental niche results in a number of uncommon genes stranded at any particular time point in any given strain. Evolutionary processes will remove genes not providing a selective advantage over time, but this may be a slow process in comparison to the acquisition of genes by horizontal gene transfer. In other words, evolutionary processes may be unable to 'empty the trash' quickly enough to eliminate all non-useful genes simultaneously. The energetics penalty imposed by a single non-useful gene is likely to be small, yet the cumulative effect of many such genes could be significant. A balance between the rate of gene acquisition by HGT and negative selection due to energetics is a likely mechanism contributing to the maintenance of the overall genome size. It is also possible that many of these unique genes are recent functional additions to the NTHi supragenome, but have not yet had time to become widely dispersed. There are a number of environmental factors that have been profoundly altered over the past half century that could account for this, including widespread antibiotic usage and high density human daycare for infants, which results in much higher rates of polymicrobial respiratory infections.
Our clustering methods were designed to minimize bias due to frame shifts and assembly gaps. Nonetheless, the number of clusters identified with these methods may contain some such bias. Sequencing errors may induce frame shifts that split a gene into two fragments. Clusters of orthologous genes (COGs) is a common method for identifying gene orthologs across a wide range of species. The COG method is able to discriminate between closely related paralogs by using only bi-direction best homology matches (BBH) while constructing clusters [28, 29]. Since the COG method requires BBH, if a split ORF is present, only one of the fragments will cluster with the full length gene. This results in orphaned 'genes', which inflate the number of gene clusters observed. To resolve this issue, we implemented a less restrictive clustering algorithm that uses uni-direction homology matches above a minimum sequence identity and a minimum fraction of the length of the shorter gene. Furthermore, six-frame gapped translations are used during homology searches to minimize the impact of sequencing errors. The disadvantage of our approach is that paralogs may cluster together if the sequence identity is above the threshold. However, since the genes under consideration are from the same species, the orthologs are expected to be highly homologous in comparison to paralogs.
Accurate clustering depends on careful selection of parameters. We started with the observation that sequence identity among orthologs is higher, on average, than among paralogs. To find the best parameters, we examined a plot of the number of clusters as a function of the parameters (Figure 1). In the case of the identity parameter, a low threshold will cause all paralogs to group together, which results in a small number of clusters. As the threshold increases, the number of clusters increases as paralogs are segregated into distinct ortholog classes. When the threshold passes the peak of the paralog distribution, the rate at which clusters split is reduced. But, as the threshold increases further, ortholog clusters begin to split, and the number of clusters increases more rapidly. At 100% identity threshold, all but the most highly conserved orthologous clusters have been split apart. Figure 1 reveals an inflection point in the region between 60% and 70% identity where the slope is decreasing and then starts to increase. The inflection point suggests that an identity threshold of 70% defines the best partition between paralogs and orthologs. Analogous reasoning was employed in determining the match length threshold.
Another bias may be introduced by the use of unfinished genomes in this study. Despite assembly gaps, the likelihood that an entire gene is missing from the sequence is low due to the high coverage (>25×, on average) generated by the 454 sequencing method. Lander-Waterman statistics predict that more than 99.9% of each genome was sequenced. Most gaps are due, therefore, not to missing sequences but rather the difficulty of assembling repeat sequences. On average, 1,769 gene clusters were found per completed genome versus 1,804 for unfinished genomes. This difference is most likely due to real genomic differences as supported by metabolomic studies (data not shown), but in the worst case the difference is an upper bound on the error.
An important consequence of our supragenome model is that the observed diversity among the H. influenzae strains can be adequately explained by a finite model. This contrasts with conclusions drawn from models built for the pathogen S. agalactiae . Our study does not contradict previous analysis, but emphasizes that conclusions are dependent on modeling assumptions and the species in question. While it is tempting to assume the supragenome of a naturally transformable species draws from the nearly infinite pool of genomic diversity found in nature, several factors make it likely the pool is quite restricted. The first barrier is environment. In the case of H. influenzae, only species that co-habitate in the human respiratory mucosa are available for genetic exchange on a regular basis. The second barrier is a set of mechanistic restrictions built into the transformation system. Uptake of DNA is enriched by the presence of uptake signal sequences, which are commonly present in H. influenzae genomic DNA but are not common in other species [30, 31]. After uptake, sequence homology is necessary for efficient incorporation of DNA into the chromosome via homologous recombination. Consequently, most HGT events among H. influenzae are expected to derive from its own population and to a lesser degree from genetically similar species residing in the same environmental niche. Our model predicts a pool of rare genes in the range of approximately 2,700 genes - this may reflect the number of genes available to the organism from genetically similar species living in the same environmental niche. This reasoning does not exclude the potential importance of rare HGT events between distantly related species on an evolutionary time-scale.
While a global analysis of the supragenome is important, the ultimate goal is an understanding of the phenotypes associated with individual genes and combinations of genes and how these contribute to the process of disease. The sequence data obtained from this study will serve as a valuable tool in this endeavor. The collection of genes identified here will be used to construct a supragenome hybridization (SGH) chip, analogous to a eukaryotic comparative genomic hybridization (CGH) chip. The SGH chip will be used as a low-cost genome screening tool for a large number of clinical NTHi isolates for which disease phenotype data are available. The resultant data will be used to generate gene association studies for the identification of genes and gene combinations that contribute to various disease processes.
The results reported herein provide evidence of a significant population-based supragenome among clinical strains of the NTHi, as well as substantive support for the DGH. The observation that, on average, every clinical strain varies from every other clinical strain by the presence or absence of over 300 genetic loci is highly suggestive that there is enormous heterogeneity among NTHi strains with respect to their pathogenic potential. These findings point the way toward future studies in which statistical genetic approaches could be brought to bear on the identification of associations between particular sets of genes within the supragenome, and the discrete clinical disease phenotypes of the individual strains. As these genic association data become available, it should be possible to develop next-generation molecular diagnostics to help with the prediction of disease treatment and outcome based upon the particular infecting population.
Complete or nearly complete genomic sequences of 11 unique clinical strains of H. influenzae were generated and used in comparative genomic analyses with the two published NTHi genomes [32, 33] in the development of a supragenome model. Genomic sequence of nine clinically isolated NTHi strains was generated at The Center for Genomic Sciences by the 454 Life Sciences GS-20 sequencer using standard protocols . Strains were sequenced to a depth of 16×, or greater, and assembled de novo by the 454 Newbler assembler to 81 contigs, on average. Lander-Waterman statistics predict that greater than 99.9% of each genome was sequenced. Regions of duplicated sequence caused most of the assembly gaps. Informal comparison between high-quality Sanger reads and 454 data suggest an error rate of less than 1 in 1,000 bases. Most base call errors are single base insertions or deletions in homonucleotide repeats that can result in frame-shift artifacts. The other two clinical NTHi isolates (R2846 and R2866) included in the comparison were sequenced at the University of Washington Genome Center (Alice Erwin, personal communication). The complete genomic sequences of H. influenzae strain Rd KW20 and 86-028NP and the incomplete sequences of strains R2846 and R2866 were accessed through the Microbial Genomes Database of NCBI.
The most recent versions of the genome assemblies were deposited with GenBank, with the following accession numbers for the indicated strains: CP000671 (CGSHiEE); CP000672 (CGSHiGG); AAZD00000000 (CGSHi22121); AAZJ00000000 (CGSHi22421); AAZF00000000 (CGSHi3655); AAZG00000000 (CGSHiAA); AAZH00000000 (CGSHiHH); AAZI00000000 (CGSHiII); and AAZE00000000 (CGSHiR3021).
Partial genomic assembly of 454-based genomic sequences
The 454-assembled PittEE strain genomic contigs were scaffolded against all four of the completed H. influenzae genomes using Nucmer , which indicated the greatest similarity to strain 86-028NP. Using a maximum parsimony approach, the PittEE genome was reduced to 12 contigs by a combination of: sequencing PCR amplicons targeted to fill gaps between neighboring contigs, as inferred by the scaffolding; and sequencing a 4 kb clone library and searching for clones that spanned gaps in the 454 sequence. Gap closure experiments were designed by a custom Perl script, and PCR primers were designed by Primer3 . Similarly, PittAA was reduced to 47 contigs by sequencing of PCR amplicons generated following scaffolding. Clones and PCR amplicons were assembled along with 454 contigs by a modified Phred-Phrap-Consed pipeline where 454 contigs were converted to PHD format files and input to Phrap as long reads [36–39].
Coding sequences for all 13 strains, including those previously annotated, were identified by the AMIgene microbial gene finder adjusted to low-GC parameters and trained on the Rd KW20 genome . AMIgene builds three Markov models to identify coding sequences with different codon usage statistics. This provides increased sensitivity for genes of possible foreign origin. Prior to gene calling, all contigs were artificially stitched together using a linker (NNNNNCATTCCATTCATTAATTAATTAATGAATGAATGNNNNN) that provided start and stop codons in all six reading frames, permitting the identification of genes that extend past the ends of a contig .
Each pair of genes was examined for protein homology by alignment of six-frame nucleotide translations to predicted protein sequences. Alignments were generated by tfasty34, part of the Fasta v3.4 package . Six-frame translations were employed to minimize the impact of frame-shift artifacts. Each gene was also aligned against the full nucleotide sequence of the 13 genomes by fasta34 (also part of the Fasta package): Fasta34 parameters, fasta34 -H -E 1 -m 9 -n -Q -d 0; Tfasty34 parameters, fasty34 -H -E 1 -m 9 -p -Q -d 0. Genes were clustered based on homology using a single-linkage algorithm. A link was defined by a significant tfasty match between genes that exceeded an identity threshold of 70% and covered at least 70% of the shorter gene (a detailed discussion of parameter selection is found in the supplementary materials at ). The asymmetric length criterion was chosen to insure that fragmented genes would cluster with the full length version of the gene. A side-effect of this criterion is that multi-domain proteins may fuse with proteins that are composed of a subset of those domains. Significant fasta matches between genes and genomic sequence were used to identify sequence conservation between a gene cluster and a strain. In the event of a significant match (70% identity/70% length), the matching genome was considered to possess the gene cluster for purposes of quantifying the number of strains that contain the gene cluster. See supplementary materials for a comparison of our clustering methods and the COG method .
Multi-alignments were generated for each cluster using poa (partial order alignment) in order to visually and computationally verify the integrity of the clusters . If the multi-alignment of a cluster was less than 120 bp in length, the cluster was filtered as a likely false-positive gene. Finally, an attempt was made to split false clusters formed by multi-domain proteins by searching for point of partition in the multi-alignment that divided the majority of genes into two non-overlapping sets. The algorithm was implemented using a custom Perl script.
Phylogenetic tree building
where gn,i= 1 if gene n is present in strain i and 0 otherwise. The strains were clustered based on the distance metric by the unweighted group average method implemented in the Phylip package [44–46]. A tree was also generated using sequence alignments of seven housekeeping genes used in multi-locus sequence typing . The tree was constructed using the maximum likelihood method implemented in fastDNAml as part of the Phylip package [48, 49].
Whole genome alignment
Whole genome alignments were generated by Nucmer and visualized by Mummerplot . MUMmer parameters were set to -maxmatch -l 16 -o. The order of PittEE contigs was inferred from optical restriction fragment maps generated by Opgen (Madison, WI, USA) . Whole genome alignments were not built for most strains since the ordering of the contigs was not determined.
Inserted and deleted genomic sequence, in comparison to the Rd KW20 genome, was identified by maximal sequence matching performed by Nucmer  with the settings -maxmatch -l 16 -o. Non-matching sequence was identified and quantified by a custom Perl script.
Multistrain local sequence alignments
Multistrain local sequence alignments against reference sequences (86-028NP or Rd KW20) were generated using BLASTn  by querying the reference sequence against a database containing the genomic sequence of all 13 strains. Alignments were then visualized using BioPerl scripts. By the nature of this alignment procedure, sequence that is present only in non-reference strains is not visualized. Gene annotations for reference strains were obtained from GenBank.
Phage homology analysis
Phage derived gene clusters were identified by selecting a representative sequence from each gene cluster to use as a BLASTx query against the NCBI NR (non-redundant) protein database. GenBank records of the top ten significant protein matches with e-value >1e-8 were queried for the keyword 'phage'. If the keyword was identified among the matches, the gene cluster was flagged as 'phage derived'.
Codon usage analysis
The codon usage of a representative sequence from each cluster was analyzed by CodeSquare using Rd KW20 mean codon usage as a reference . The epsilon statistic reported by CodeSquare was normalized for ORF length dependence using a best-fit power function for the mean and variance (as a function of length). Gene clusters were divided into three categories: core (gene found in all 13 strains), contingency (2-12 strains), and unique (1 strain). To minimize length bias, codon usage analyses were limited to genes with lengths between 200 and 300 amino acids. Significant differences in the median epsilon statistic were calculated using the non-parametric Mann-Whitney U test.
The authors thank N. Luisa Hiller for valuable discussions and data checking; Alice Erwin and Arnold Smith of the Seattle Biomedical Research Institute and Maynard V Olson, Rajinder K Kaul and Yang Zhou of the University of Washington Genome Center for sharing the completely assembled sequences of the NTHi strains R2846 and R2866 in advance of publication. NTHi strain 3655 isolated from a patient with otitis media was provided by Allen Ryan at UCSD. This work was supported by Allegheny General Hospital, Allegheny Singer Research Institute, Seattle Biomedical Research Institute, and grants from the Health Resources and Services Administration and the NIH-NIDCD: DC02148 (GDE), DC04173 (GDE), DC00129 (AR) and DC05659 (JCP). The authors thank Mary O'Toole for help with the preparation of this manuscript.
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