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Table 3 Differential expression data based on array experiments and Q-PCR of genes with unknown or ubiquitous expression pattern

From: A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks

Other genes Expression Regulation P Value Q-PCR ratio
Arginine kinase
536REAsu4_15B20
? Li 2.20
Zn na
7.29 × e-07
na
1.95 ± 0.15
2.07 ± 0.47
ß-catenin
538REA_2O22 RUDIREA_22E13
Ubiquitous Li 0.39
Zn na
4.77 × e-03
na
0.85 ± 0.11
0.79 ± 0.16
SpZ12 ? Li 1.44
Zn 2.29
2.55 × e-01
2.05 × e-02
2.03 ± 0.37
2.66 ± 0.40
Hmx, NkX5.1
537REA_6C02
? Li 0.20
Zn 1.86
1.18 × e-14
5.87 × e-03
0.05 ± 0.03
1.20 ± 0.32
Tcf/Lef
536REAsu4_11P24
? Li 1.37
Zn na
7.88 × e-02
na
0.73 ± 0.13a
1.42 ± 0.29
WntA
RUDIREA_33L4
? Li 1.07
Zn nd
8.13 × e-01
nd
0.39 ± 0.13a
0.24 ± 0.12
Wnt1 ? Li na
Zn na
na
na
ne
ne
Wnt4
536REAsu4_6C19
? Li 1.09
Zn NA
6.69 × e-01
NA
1.66 ± 0.36
1.73 ± 0.21
Wnt6 ? Li na
Zn na
na
na
1.55 ± 0.19
0.24 ± 0.01
Wnt7 ? Li na
Zn na
na
na
ne
0.03 ± 0.02
Wnt9 ? Li na
Zn na
na
na
ne
0.29 ± 0.03
Wnt10 ? Li na
Zn na
na
na
1.43 ± 0.29
1.30 ± 0.14
Wnt16 ? Li na
Zn na
na
na
2.79 ± 0.81a
0.84 ± 0.12
  1. The first column gives the gene name and the clone ID, both of which can be used to query the described database [22] for additional data. In the second column the localization of expression in the embryo is given. The third column (Regulation) gives the differential expression ratios (expression in treatment/expression control) based on the array experiment for lithium (Li) and zinc (Zn) treated embryos (values above 1 indicate upregulation and values below 1 indicate downregulation). The column 'P value' indicates the statistical probability that the regulation could happen by chance (see Materials and methods for detail). The column Q-PCR (quantitative real-time polymerase chain reaction) gives the differential expression ratios (expression in treatment/expression control) and the error, as determined by Q-PCR. (Values expressed in copies of mRNA molecules/embryo are provided via the expressed sequence tag database [75]; see Materials and methods for details on Q-PCR). aDifferential expression based on array and Q-PCR data do not correlate. na, not analyzed; nd, no statistically relevant differential expression; ne, not expressed; ?, expression pattern unknown.