Figure 3

The predicted specificity residues of the human Ras family map to known functional sites in 3D. The specificity residues (marked '#' in Figure 2), such as the switch I and II regions, are separated along the sequence, but end up in functional positions near the active site, poised to modulate the interaction with protein partners such as the guanine nucleotide exchange factor Sos (colors as in fig. 2). Because the computation of specificity residues uses no information about known three-dimensional structures, molecular complexes, or interactions, the agreement between the computed specificity residues and their location in the experimentally observed interfaces illustrates the predictive power of the method.