Figure 4

Gene neighborhoods of prokaryotic ThiS/MoaD-like ubiquitin domains and functionally associated proteins. Genes found in conserved neighborhoods are depicted as boxed arrows with the arrow head pointing from the 5' to the 3' direction. ThiS/MoaD-like proteins are shaded in blue. Other than in the classical ThiS and MoaD pathways, ThiS/MoaD/Ubiquitin-like proteins are labeled Ubl for ubquitin-like domain. The ThiS/MoaD-like proteins in each operon are identified in black lettering below the neighborhood by gene name, species abbreviation and gi number, demarcated by underscores. In the instances where ThiS/MoaD-like domains are absent, the gene neighborhoods are identified by the JAB domain containing protein. Alternative names of experimentally well characterized genes are shown below the boxed arrows for that gene. Boxed arrows with no colors represent poorly conserved proteins. Conserved neighborhoods are clustered according to major assemblages of gene neighborhood as described in the text. In Sulfolobus MoaD and MoaE are intriguingly linked to ThiD, but any possible role in thiamine biosynthesis remains unclear. Species abbreviations are listed in the legend to Figure 2. AOR, aldehyde ferredoxin oxidoreductase; Cys Synthase, cysteine synthase; PE, PE family of proteins; PPE, PPE family of proteins;Rhod, Rhodanese domain; Z, poorly characterized protein with an α + β domain with several conserved charged residues; X, β-strand rich globular domain; YueB, bacillus YueB-like membrane associated protein.