- Open Access
RNA interference has a role in regulating Drosophilatelomeres
© BioMed Central Ltd 2006
- Published: 31 May 2006
Unlike many other organisms, Drosophila maintains its telomeres by the transposition of retrotransposons to chromosome ends. Recent work shows that proteins in the RNA interference pathway specifically regulate the expression of these retrotransposons and frequency of transposition in germline cells, but do not affect retrotransposon expression or telomere function in the soma.
- Transposable Element
- Telomere Length
- Nurse Cell
- Germline Cell
- Telomere Maintenance
Although the mechanism of transposition of non-LTR transposons is well understood, there is still much to learn about how the addition of retrotransposon elements to the telomere array is regulated. A recent paper by Savitsky et al.  provides intriguing evidence that proteins associated with RNA interference (RNAi) may be involved. Earlier work from this same group  had shown that mutant alleles of two genes encoding components of the RNAi machinery lead to increased accumulation of RNA from several transposable elements in female germline cells . These two genes, spindle-E (spn-E) and aubergine (aub), respectively encode a DEAD-box helicase  and the Aub protein, a member of the Argonaute family that is necessary for assembly of the RNA-induced silencing complex (RISC) . The RNAi machinery is generally thought to have evolved to help cells battle the invasion of parasitic elements, so the increased accumulation of transposable elements in cells with defective RNAi genes was not surprising. It was surprising, however, that one of the transposable elements in this study was HeT-A, a transposable element dedicated to telomere maintenance in Drosophila, not a parasitic invader. Savitsky and colleagues  have now extended these studies to show that the RNAi genes are indeed acting on components of the telomere.
Savitsky et al.  have now further explored the effects of spn-E and aub mutations on telomere maintenance, and find evidence that these two genes have a regulatory role. The mutant phenotypes indicate that these genes are involved in controlling the flow of telomere elements to the ends of chromosomes. The results of the study show that this control is complex, affecting the telomere-specific elements in different ways.
Apart from HeT-A, the two other telomere-specific retro-transposons are TART and Tahre. Savitsky et al.  show that the expression of TART is also affected by mutant alleles of spn-E and aub. Tahre may be affected but, because it shares so much of the HeT-A sequence , it would be included in the HeT-A results in these studies. The mutations studied affect HeT-A and TART differently. Firstly, although both RNAs were upregulated specifically in germline cells, they had different patterns of accumulation. The increase in TART RNA was detected in the nurse cells in the later stages of oogenesis. In contrast, increased levels of HeT-A transcripts were detected in oocytes even in early stages and were then seen later in nurse cells. Secondly, the effect of mutation on HeT-A expression was less robust than on TART, with all mutant alleles increasing TART expression but some having no effect on HeT-A. For example, several aub alleles increased HeT-A expression in only a fraction of the ovaries, or sometimes only in some ovarioles in a single ovary. Only in one case was HeT-A expression increased to a higher level than TART: in homozygous spn-E1 flies TART abundance is about twice that seen in heterozygotes, whereas HeT-A is over ten times more abundant.
The differences in the response of HeT-A and TART might seem surprising, but they are less so when considered in the light of other differences between these two elements. Indeed, the two retrotransposons are regulated quite differently in non-mutant flies. HeT-A is expressed only in diploid cells, predominantly in S-phase cells, and yields few, if any, antisense transcripts. TART is expressed in both diploid and polyploid cells and produces much more antisense than sense RNA . The effects seen by Savitsky et al.  now show that the regulation of HeT-A and TART expression by RNAi is also different.
Increased expression of HeT-A and TARTcorrelates with increased transposition to telomeres in the germline
Using this system, Savitzky et al.  clearly show that the increased transcription in the mutant flies is correlated with an increased frequency of transposition of the retrotransposons and consequently in telomere elongation. For non-mutant flies the frequency of transposition onto the broken end was 0.04%. In flies carrying a single copy of either a spn-E or an aub mutation, this rate was increased by between 20- and 100-fold or more, depending on the allele. Surprisingly, more than 95% of the new transpositions were of TART, whereas the majority of transpositions in wild-type flies are of HeT-A [9, 10]. Only in homozygous spn-E1 flies, where HeT-A expression is dramatically increased, is HeT-A transposition more frequent than that of TART. Clusters of progeny with identical TART attachments indicated that at least some of the transposition occurred in premeiotic cells. These results add to the increasingly complex picture of regulation of the telomeric retrotransposons.
Savitsky et al.  show that the spn-E and aub mutations lead to increased HeT-A and TART expression, which correlates with their increased rate of transposition to chromosome ends. But it is important to note that despite the greatly increased frequency of attachment of HeT-A and TART to broken ends observed in this assay, lines heterozygous for spn-E or aub mutations do not have detectably greater numbers of HeT-A and TART in their genomes . This suggests that there are further levels of regulation of telomere elongation involving additional important players. One explanation might be that the mechanism of elongation studied here could act only on broken chromosome ends. On the other hand, it could be acting on all chromosome ends, including unbroken telomeres. The frequency of retrotransposon addition in mutant flies is low enough for it to take generations to make a significant change in the very long Drosophila telomeres, even though addition is easily detected with the powerful screen of a broken chromosome. Similarly, the lack of an RNAi effect on either expression of HeT-A or TART  or telomere fusions  in somatic tissues indicates that regulation of telomere length in somatic tissues is independent of RNAi, or at least does not involve the RNAi genes studied here.
Earlier work has shown that mutations in the gene for HP1, a chromatin protein, also increase both the abundance of HeT-A and TART RNA and their frequency of transposition to broken ends . In contrast, loss of one copy of either of the DNA repair genes Ku70 or Ku80 strongly increased transposition to broken ends but did not increase expression of HeT-A  (expression of TART was not reported). Taken together, these observations suggest that there may be different pathways of telomere length regulation that may be specific to different cells and different types of telomere defects.
The work of Savitsky et al.  shows clearly that products of spn-E and aub are involved in regulating the expression and transposition of HeT-A and TART. The mechanism of this regulation is not yet determined but, because both spn-E and aub are components of the RNAi-based silencing mechanism , it is likely that RNAi is involved. This possibility is supported by the evidence that short (26-29 nucleotide) RNAs with HeT-A and TART sequences are found in wild-type flies and flies heterozygous for a spn-E mutant allele, but are absent in flies carrying two spn-E mutant alleles . Short RNAs of this size have been shown to be involved in transcriptional silencing in plants , Caenorhabditis elegans  and mammals , and in genome rearrangements in Tetrahymena , suggesting that RNAi is affecting Drosophila telomeres by acting on the chromosome rather than on an RNA transcript.
On their evidence that the regulation of TART is more sensitive to disruptions in the RNAi pathway than is HeT-A regulation, Savitsky et al.  suggest that TART is the principal target of RNAi regulation in the germline. This is based on the observation that heterozygous mutations induce much more transposition of TART than of HeT-A. This contrasts with the situation in wild-type flies, however, where HeT-A transpositions to broken ends are much more frequent than TART transpositions [9, 10]. In addition, HeT-A is significantly more abundant than TART in the genomes of stocks that have been examined (P.G. DeBaryshe, personal communication). The predominance of HeT-A has always been puzzling, because HeT-A is an exceptional retrotransposon in that it does not encode the Pol protein, which provides enzymatic activities such as reverse transcriptase needed for retrotransposition. TART does encode Pol and the possibility that TART supplies Pol for HeT-A is supported by evidence that HeT-A Gag protein localizes TART Gag to telomeres . The finding that TART is the more sensitive RNAi target opens new avenues to explore the collaboration of HeT-A and TART that is seen in all Drosophila species .
One result from the Savitsky et al. study  suggests that the collaboration between HeT-A and TART may not be simple. The dramatic increase in HeT-A RNA and the predominance of HeT-A transposition seen in homozygous spn-E1 flies shows a nice correlation between expression and transposition frequency, but it raises questions about the mechanism by which gene dosage can affect the dominance relationship of HeT-A and TART.
One of the unusual features of TART is the production of abundant antisense RNA. This feature is conserved in all Drosophila species, suggesting that the antisense RNA is important for telomere maintenance or regulation . Surprisingly, spn-E and aub mutants did not affect the expression of antisense TART RNA. Because there is a decrease in the levels of short TART RNAs (see above), an increase in TART antisense RNA should be expected. The failure of Sav-itsky et al.  to find any effect on the expression of this RNA could indicate that TART antisense RNA makes only a minor contribution to double-stranded RNA production. If so, this would set some limits on antisense functions. Perhaps TART antisense RNA acts in a different pathway of regulation, such as the somatic regulation of telomere length, or in a different aspect of telomere maintenance, such as epigenetic control of the heterochromatic structure of the telomeres.
HeT-A and TART have split personalities. They have the hallmarks of non-LTR retrotransposons but at the same time these two elements have been dedicated to telomere maintenance throughout the more than 60 million years since the separation of the genus Drosophila . Their regulation by the RNAi machinery could simply be a reflection of their retrotransposon nature. On the other hand, there is recent evidence that RNAi regulates telomere activity in organisms that have telomerase; mutations in the RNAi machinery have been shown to disrupt telomere function in both Schizosaccharomyces pombe  and Tetrahymena . Perhaps this is another case where the variant Drosophila telomere is sharing in a general cellular mechanism.
Finally, it is important to keep in mind that, in addition to the effect on transposition onto broken chromosome ends in germline cells demonstrated by Savitsky et al. , the RNAi regulation of HeT-A and TART might have other functions yet to be discovered. Mammalian telomerase has other roles, still not completely understood, that are independent of telomere elongation . Once again, studying Drosophila telomeres, apparently so different, might shed light on possibly important features of other eukaryote telomeres.
This work has been supported by National Institutes of Health Grant GM50315 to M.L.P. We thank M.L Espinàs, D. Huertas and F. Azorín for critical reading of the manuscript.
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