- Open Access
Multiple independent evolutionary solutions to core histone gene regulation
Genome Biology volume 7, Article number: R122 (2006)
Core histone genes are periodically expressed along the cell cycle and peak during S phase. Core histone gene expression is deeply evolutionarily conserved from the yeast Saccharomyces cerevisiae to human.
We evaluated the evolutionary dynamics of the specific regulatory mechanisms that give rise to the conserved histone regulatory phenotype. In contrast to the conservation of core histone gene expression patterns, the core histone regulatory machinery is highly divergent between species. There has been substantial evolutionary turnover of cis-regulatory sequence motifs along with the transcription factors that bind them. The regulatory mechanisms employed by members of the four core histone families are more similar within species than within gene families. The presence of species-specific histone regulatory mechanisms is opposite to what is seen at the protein sequence level. Core histone proteins are more similar within families, irrespective of their species of origin, than between families, which is consistent with the shared common ancestry of the members of individual histone families. Structure and sequence comparisons between histone families reveal that H2A and H2B form one related group whereas H3 and H4 form a distinct group, which is consistent with the nucleosome assembly dynamics.
The dissonance between the evolutionary conservation of the core histone gene regulatory phenotypes and the divergence of their regulatory mechanisms indicates a highly dynamic mode of regulatory evolution. This distinct mode of regulatory evolution is probably facilitated by a solution space for promoter sequences, in terms of functionally viable cis-regulatory sites, that is substantially greater than that of protein sequences.
Core histone genes encode four families of proteins that package DNA into the nucleosome, which is the basic structural unit of eukaryotic chromosomes . The four core histones are H2A, H2B, H3 and H4, and each nucleosome consists of 146 base-pairs (bp) of DNA wrapped around an octameric core containing two copies of each histone protein. Comparative studies of core histones have revealed that their sequences are among the most evolutionary conserved of all eukaryotic proteins . For instance, the human H4 protein (NP_003539) is 92% identical to its yeast Saccharomyces cerevisiae ortholog (NP_014368) . The high levels of core histone sequence conservation are thought to be due to severe structural constraints imposed by their assembly into the histone octamer  as well as the similar functional constraints across species associated with the compact binding of DNA .
Most of the packaging of genomic DNA by core histones occurs primarily during the S phase of the cell cycle, when DNA is being actively replicated; stoichiometrically appropriate levels of histone proteins are required to bind DNA immediately following replication . As such, the expression of core histone genes is tightly regulated and peaks sharply during S phase . Much like the histone sequences, this histone gene expression pattern is highly conserved among eukaryotes ranging from human to the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe [8–13].
The mechanisms that underlie the cell cycle specific regulation of core histone genes have been intensively studied [6, 7]. Although most of this work has focused on the regulation of transcription via the interaction of cis-regulatory elements and transcription factors, a number of studies have also addressed the role of post-transcriptional regulation of core histone synthesis. Here, we focus exclusively on the regulation of core histone gene expression at the transcriptional level. Numerous studies have characterized core histone cis-regulatory sites and their cognate transcription factors [7, 14–23]. Sequence logos representing 14 experimentally verified cis-regulatory motifs, along with the names of the transcription factors that bind them, are shown in Figure 1.
The studies that resulted in the characterization of these motifs and transcription factors have led to the elucidation of core histone gene regulation in model experimental systems such as S. cerevisiae. For example, the yeast transcription factor Spt10p was recently demonstrated to activate core histone gene expression . Interestingly, the SPT10 gene was originally identified as a suppressor of Ty insertion mutations [24, 25] and as a global regulator of core promoter activity . However, despite the fact that Spt10p affects the expression of hundreds of yeast genes, it specifically binds cis-regulatory sequences, referred to as upstream activating elements, which are found only in core histone gene promoters. Thus, the global regulatory properties of Spt10p are based solely on changes in levels of core histone gene expression. In support of this model of histone gene regulation, the DNA-binding domain of Spt10p was recently characterized and shown to mediate sequence-specific interaction with the core histone gene upstream activating element . There are a number of such examples, from S. cerevisiae and other model systems, of efforts to characterize experimentally the mechanisms of core histone gene regulation. In addition, efforts are underway to investigate core histone promoters among different species computationally .
Despite the substantial body of knowledge on the regulation of core histone genes, little is known about the evolutionary dynamics that have given rise to these regulatory mechanisms. We present here an evolutionary analysis of core histone gene regulatory mechanisms. The emphasis of this work is placed on understanding the evolution of cis-regulatory sites along with their cognate transcription factors. We analyzed the phyletic distributions of 14 experimentally verified core histone cis-regulatory elements among 24 crown group eukaryotes. The evolution of core histone gene cis-regulatory sites and transcription factors is considered in light of core histone protein sequence and structure evolution. Despite the highly conserved core histone sequences and expression patterns, the mechanisms of histone gene regulation were found to be highly divergent and lineage specific. The implications of this dissonance with respect to the evolution of gene regulatory systems are explored.
Results and discussion
Gene expression patterns
The expression of core histone genes is tightly regulated during the cell cycle and peaks specifically during S phase, concomitant with DNA replication (Figure 2). This is thought to be due to the requirement for histone proteins to bind DNA immediately after its synthesis. A number of recent studies have revealed the extent to which this S phase specific pattern of core histone gene expression is conserved among eukaryotic species; the histone expression pattern has been demonstrated for human core histone genes as well as for histones from S. cerevisiae and S. pombe [8–13]. This highly conserved regulatory phenotype (the expression pattern) is consistent with the deep conservation of histone protein sequences and further underscores the strong functional (selective) constraint that histone genes are subject to. Considering the highly conserved regulatory phenotype of core histone genes, it would seem to follow that their regulatory mechanisms are similarly conserved.
Lineage-specific cis-regulatory mechanisms
Contrary to the expectation that core histone genes would have conserved regulatory mechanisms across species, the best studied core histone genes - namely human and S. cerevisiae (yeast) - have different promoter architectures; in fact, they are regulated quite differently . The human and yeast core histone promoters, many of which are bidirectional, are illustrated in Figure 3. Human core histone gene promoters contain more known cis-regulatory binding sites, relative to yeast promoters, which is consistent with the involvement of more transcription factors and the greater complexity of human histone gene regulation. Out of the 14 experimentally characterized cis-regulatory sites that are known to be involved in histone gene regulation in the two species, only one site, the TBP/TATA box, is shared between the two species (Table 1). Furthermore, the phyletic distributions (the presence/absence among species) of the trans-regulatory binding proteins that interact with these sites tend to be lineage specific (Table 2).
In order to evaluate the evolution of core histone promoter cis-regulatory sites in more detail, the phyletic distribution of all 14 experimentally characterized DNA binding motifs among 24 crown group eukaryotic species was assessed. To do this, position frequency matrices (PFMs) of the cis-regulatory motifs (Figure 1) were taken from the TRANSFAC database  or were generated from the binding site alignments reported in the original citation. Intergenic promoter regions of core histones (H2A, H2B, H3, and H4) for all 24 species were then searched for the presence of the 14 cis-regulatory motifs using the program CLOVER . CLOVER uses the cis-regulatory site PFMs to evaluate the promoter sequences for statistically significant over- or under-representation of motif elements. For any given promoter sequence (P i ), CLOVER assigns a numerical value (raw score) to each cis motif (j) indicating its over- or under-representation in that sequence. The distribution of cis-regulatory motifs in that promoter is then represented as a vector, P i = (P i1, P i2 ... P i14], of sequence- and motif-specific CLOVER scores (P ij ). The CLOVER-generated vectors were then compared using the Pearson correlation coefficient (r). High r values would thus represent two promoter sequences with similar cis-regulatory binding sites. The r values were transformed into pair-wise promoter distances using the following formula: d = 1 - (r + 1)/2.
A total of 254 core histone promoter sequences were compared in this way, resulting in a matrix of 32,131 pair-wise distances. This distance matrix was evaluated using a fast implementation of the neighbor-joining algorithm [31, 32] to determine the evolutionary relationships, based on cis-regulatory binding sites, among the core histone promoter sequences (Figure 4). Surprisingly, when histone promoter sequences are related in this way, they tend to form clusters that are relatively lineage specific with respect to the species from which they are derived rather than their family of origin. For instance, there are fairly well defined clusters of histone promoters that are fungi specific and others that are metazoan specific (see red blocks in Figure 4). Importantly, these distinct clusters contain promoters from all four histone gene families. In general, histone promoter sequences from different families are completely intermixed on the tree (they do not tend to group into gene family specific clusters). This suggests that some core histone promoter regions may be evolving in concert within evolutionary lineages, perhaps due to similar lineage-specific regulatory constraints.
The lineage-specific nature of core histone promoter sequence evolution was further explored by generating a species distance matrix analogous to the sequence distance matrix described above. For the species distance matrix, CLOVER scores were calculated for sets of all promoter sequences from individual species. Species-specific CLOVER vectors calculated in this way were compared using r value distances, and the resulting distance matrices were used to compute a neighbor joining tree (Figure 5a). As a control, the same comparison was done using promoter sequences that were randomly permuted with preservation of their mono- and dinucleotide frequencies (Figure 5b). Although the topology of the control tree shows no relationship to the species phylogeny, the topology of the tree generated from the observed data is in general agreement with the species phylogeny and thus underscores the within-species coherence of the core histone promoter cis-regulatory motifs. There are, however, some interesting exceptions to this trend. For example, S. pombe and Aspergillus nidulans are found in a cluster that includes Drosophila mojavensis; in addtition, Arabidopsis thaliana is nested close to vertebrates as opposed to being an outgroup to the entire ensemble, as would be expected.
Motif evolutionary dynamics
Further examination of the cis-regulatory motif distribution within the yeast group of species (order Saccharomycetales) shows that different combinations of motifs have distinct evolutionary trajectories, suggesting lineage-specific mechanisms of regulation (Figure 6). For instance, Spt10p and TBP combine to regulate core histones among all Saccharomycetales species evaluated here, whereas the NEG element exerts its negative regulatory effects exclusively among S. cerevisiae and its two closest relatives. Furthermore, the position-specific sequence conservation of cis motifs is coherent within species but divergent between species. The information content along positions of the motif sequences changes slightly between lineages, and visual inspection of these changes suggest that they are not always in accordance with the phylogenetic relationships among species (Figure 6).
The position of cis-regulatory motifs in the proximal promoter sequences is also critical to histone gene regulation as demonstrated by the conserved relative positions of the motifs in particular species contexts (Figure 7). Spt10p has four experimentally characterized binding sites for each bidirectional promoter in S. cerevisiae (Figure 3). Accordingly, when the relative position of Spt10p cognate sequence motifs are evaluated among all species where they are present, they exhibit a marked clustering in the center of the promoter regions (compare Figure 7 panels a and b). On the other hand, NEG and TBP are excluded from the centers of the core histone promoters and tend to map closer to the translational start sites (Figure 7c-f).
Sequence and structure evolution
The lineage-specific pattern of core histone promoter evolution revealed by the comparative analysis of cis-regulatory motif sequences stands in contrast to the evolution of core histone protein sequences and structures. There are four families of core histone proteins, namely H2A, H2B, H3 and H4, and these families are present in all eukaryotes, indicating that they probably evolved via three ancient gene duplication events that preceded the diversification of the eukaryotic lineage. Given this evolutionary scenario, it can be expected that all protein sequences (structures) of a given family will be more closely related to one another, regardless of the species from which they are derived, than they are to members of other families. Straightforward sequence comparison methods, such as BLASTP , bear this expectation out (data not shown). In fact, although sequences within families are highly conserved, it is not possible to identify members of different families using pair-wise BLASTP comparisons. On the other hand, despite its low sequence similarity among core histones, the histone fold domain (HFD) is present in all four core histones [34, 35].
In order to explore the sequence/structure relationships within and among core histone protein families, sensitive methods of comparison are needed. For instance, comparisons of three-dimensional protein structures  can often reveal deep evolutionary relationships that are not apparent when protein sequences alone are compared. A high-resolution structure of the Xenopus laevis nucleosome exists, and structural comparison of the individual histone units, which correspond to distinct histone families, was performed using similarity scores from the DALI database . The statistically significant similarity scores observed indicate that the signal of common ancestry among all histone families is preserved at the structural level. For each histone variant, its pair-wise DALI Z scores were normalized by the self-comparison of Z scores (Z ij /Z ii ) to yield a relative Z score (Z r ), and the distance was taken as d = 1 - z r . The resulting pair-wise distance matrix was used to build a neighbor joining tree for the four histone families (Figure 8a). This tree shows that H2A and H2B form one related cluster, whereas H3 and H4 form another. Interestingly, these evolutionary relationships are reflected in the structure (Figure 8b) and assembly dynamics of the histone octamer . H3 and H4 first form dimers that come together as a tetramer. Meanwhile, H2A and H2B form dimers separately and these H2A-H2B dimers join the H3-H4 tetramer to form the octamer.
A more detailed analysis of the evolutionary relationships within and between histone protein families was performed using a comparative analysis of the HFD. The HFD is represented in the Pfam database, and an alignment of its representative members has been used to generate a hidden Markov model (HMM) that captures the position-specific sequence variation characteristic of the domain. In order to build a multiple sequence alignment that unites members of all four families, representative members of each family from the 24 species analyzed here were aligned in register to the HFD-HMM. This HFD multiple sequence alignment was then used to calculate all pair-wise distances, within and between families, and to build a HFD phylogeny (Figure 8c). As expected, all members within any given family are more closely related to one another than to members of any other family. The phylogenetic relationships within families are largely consistent with the established taxonomic relationships of the species from which the sequences were derived. However, the relatively high within-family sequence identities, as well as the level of resolution afforded by the between-family HMM approach, do not lend themselves to robust delineation of evolutionary relationships within families. Perhaps most germane is the fact that the between-family relationships illustrated by the HFD-HMM approach are identical to those seen in the DALI structural comparison. It is worth reiterating that these family-specific protein sequence relationships are totally discordant with the largely lineage-specific promoter sequence element relationships.
We have demonstrated a striking dissonance between the deep evolutionary conservation of core histone regulatory phenotypes and the profound divergence of their regulatory mechanisms. Core histone genes exhibit similar cell cycle (S phase specific) expression patterns from the yeast S. cerevisiae to human (Figure 2). This regulatory conservation is consistent with the high levels of sequence conservation among core histone proteins. Nevertheless, the regulatory mechanisms that are used to achieve the conserved expression patterns of core histone genes are almost entirely lineage specific. The cis-trans machinery involved in core histone gene regulation has changed substantially between lineages through gain and loss of transcription factor proteins and their cognate binding sites. This suggests that, for families like the core histone genes, phylogenetic footprinting  may have limited utility for identifying functional regulatory elements across all but the most closely related species.
In addition to the divergence of cis sites and trans factors, a distinct level of post-transcriptional regulation of core histones emerged along the metazoan evolutionarily lineage . Core histone gene 3'-untranslated regions encode a stem loop structure (Figure 9a) that, when bound by protein, greatly increases mRNA stability. This mechanism is responsible for 70% of the upregulation of core histones in S phase. The sequence that forms the stem loop is conserved across metazoans (Figure 9b). The emergence of this mechanism may have allowed for some of the turnover of the cis-trans regulatory machinery among metazoan genomes subsequent to their divergence from the yeast evolutionary lineage.
There are additional regulatory elements that may help to achieve coordinated regulation of core histone genes in metazoans. For instance, a sequence found in core histone gene encoding regions is important for their expression and may serve as an internal promoter element common to the mammalian lineage [41–43]. In addition, the transcription factor NPAT has been implicated as a global regulator of core histone gene expression among metazoans even though it does not seem to bind any DNA sequence directly [44–46]. This may provide yet another global lineage specific regulatory mechanism that distinguishes the metazoan mode of core histone gene regulation from that of yeast.
Even though the four core histone gene families (H2A, H2B, H3, and H4) diverged before the species studied here, the regulatory mechanisms are more similar for different family members within species than for the same family members between species (Figures 4 and 5). Thus, there is a kind of concerted regulatory evolution operating between members of different core histone gene families. This pattern stands in stark contrast to the pattern of core histone sequence evolution, whereby members of the same family are more similar to one another across species reflecting their more recent common ancestry (Figure 8). This suggests that very different modes of evolution exist for histone gene regulation versus protein sequence and structure. The solution space for promoter sequence evolution (the space of functionally viable cis-regulatory binding site sequences) may be far more vast than that of core histone protein sequences. This results in a much more dynamic evolutionary paradigm for promoter sequences and the transcription factors proteins that bind them. Purifying selection may be less efficacious at eliminating variants of cis-regulatory sites because a number of sequence variants may bind transcription factors with similar affinities. In addition, new cis-regulatory sites, which are short and degenerate by nature, may arise relatively quickly through mutation along the promoter. It is possible that these new variants can lead to an exploration of expression space and rapid fixation of adaptive variants by positive selection. Adaptive expression changes of this type may be facilitated by the emergence of intermediate redundant regulatory programs that maintain the ancestral expression pattern and function while simultaneously allowing for selective testing of novel expression patterns . Such an evolutionary mode, with less pronounced purifying and more prominent adaptive selection, could explain the observation that novel cis-trans combinations are subject to substantial turnover and may be regularly reinvented among evolutionary lineages. In addition, the inherent evolutionary flexibility of regulatory systems may allow for coordinated within-species changes that respond to epistatic pressure from other regulatory pathways in the same lineage that share transcription factors.
It is currently unclear whether the turnover of regulatory mechanisms, in the face of conserved expression patterns, is unique to core histones or also occurs for other gene families. Some studies on the evolution of gene regulation do report evidence of conserved regulatory sequences and expression patterns [47, 48], whereas others indicate that gene regulatory networks do in fact diverge rapidly [49–51]. However, regulatory divergence usually leads to distinct expression patterns [51–53]. Interestingly, although yeast core histone transcripts include polyA tails, core histone transcripts are unique among metazoan transcripts in that they lack polyA tails. The absence of polyA tails, which are often bound by poly(A)-binding proteins to promote translation initiation, may necessitate, to some extent, species-specific solutions to core histone gene regulation.
The comparative genomics of core histone gene regulation reveal a novel evolutionary mode, which we dub 'circuitous evolution'. Circuitous evolution of core histone gene regulation is distinct from convergent evolution, because the conservation of the core histone gene regulatory patterns suggests that the same pattern existed in the last common ancestor of all species analyzed here. After divergence from the last common ancestor, the core histone expression patterns remained unchanged but the regulatory mechanisms that give rise to the conserved phenotype diverged dramatically. Thus, with respect to core histone gene regulation, where you are from and where you are are far more important than how you get there.
As an addendum, during revision of the manuscript we became aware of a recently published paper , which confirms that the specific periodic pattern of core histone gene expression is uniquely evolutionarily conserved. The report by Jensen and coworkers also demonstrates how many different regulatory solutions have evolved to control the periodic expression of integrated biological systems that function in the cell cycle.
Materials and methods
Core histone protein coding sequences were obtained from the histone database . A list of species from which the sequences were obtained is provided in Additional data file 3. Core histone protein coding sequences were used as queries in a series of tblastn  searches against species-specific National Center for Biotechnology (NCBI) Entrez Genome project databases  in order to locate the precise genomic regions of core histones. Entrez Genome project species-specific databases include complete Reference Sequence (RefSeq) genomes when available or whole genome shotgun sequence entries when RefSeq versions are unavailable. Core histone proximal promoter sequences were taken as 1 kilobase upstream of the annotated translational start site. For bidirectional promoters, the entire intergenic regions (range 133 to 970 bp; average 413 bp) were taken for analysis. Promoter sequences are provided as Additional data file 1. The nomenclature reported by Marzluff and coworkers  was used for the human and mouse core histone genes.
Cis-regulatory binding sites
The DNA binding subunits of the transcription factor proteins and their cognate cis-regulatory binding sites were taken from the published literature as described in the Introduction and Results and discussion sections. PFMs of the cis-regulatory motifs were taken from the TRANSFAC database  or, when not available, generated from the binding site alignments reported in the original citation. The PFMs were used with the program CLOVER  to search the core histone promoter regions for the presence of the cis-regulatory motifs. The complete set of CLOVER predictions is provided in Additional data file 4. CLOVER output was used to construct promoter-specific vectors composed of scores of over-represented and/or under-represented cis sites for each sequence; the vectors were then used to compare promoter sequences with pair-wise Pearson correlation coefficients. CLOVER also gives the position of each predicted motif and these positions were normalized by the length of the promoter sequence to give the relative lengths shown in Figure 7 panels a, c and e. Locations were randomly sampled from a uniform distribution in order to generate the negative control plots shown in Figure 7 panels b, d and f.
The TFBS Perl modules  were used to further analyze cis-regulatory sequence binding motifs. For each cis-regulatory motif, sequences of all the motif sites predicted by CLOVER were extracted and aligned. These alignments were used to construct PFMs, which were converted to position weight matrices by normalizing with background nucleotide frequencies. Information content per cis-regulatory sequence motif position , taken from the position weight matrices, were used to build sequence logos with the program WebLogo .
Protein sequence and structure
Core histone protein sequences were taken from the histone database . Protein sequences are provided in Additional data file 2. A probabilistic HMM representing the HFD found in all core histone proteins [34, 35] was taken from the Pfam database . The HFD (Pfam accession number: PF00125) was extracted from each core histone protein sequence using the HMM with the program HMMER . The core histone HFD multiple sequence alignment was built by aligning each HFD sequence back to the PF00125 HMM, thus preserving the same structural register for all HFD domain sequences. The program QuickTree  was used to build a neighbor joining tree  from the HFD multiple sequence alignment.
A three-dimensional structure of the nucleosome core, PDB ID:1KX5 , used for comparison was taken from the RCSB Protein Data Bank . Structural comparisons between the individual core histone proteins were performed using the fold classifications computed in the Dali database [37, 64]. Z scores between individual core histone proteins were taken and converted to pairwise distances (d) by normalizing with the self-similarity Z score using the following equation:
Gene expression data were taken from reports published elsewhere [8–13]. For Figure 2, relative expression levels (log2 ratios) for S. cerevisiae were plotted against cell cycle time points, and visualization was done using matrix2png .
Additional data files
The following additional data are available with the online version of this article. Additional data file 1 contains the promoter sequences of core histone genes used in the study. Additional data file 2 contains the core histone protein sequences used in the study. Additional data file 3 contains the list of species used in the study. Additional data file 4 contains the CLOVER predictions for all core histone gene promoters used in the study.
van Holde KE: Chromatin. 1989, London: Springer-Verlag
Malik HS, Henikoff S: Phylogenomics of the nucleosome. Nat Struct Biol. 2003, 10: 882-891. 10.1038/nsb996.
Marino-Ramirez L, Hsu B, Baxevanis AD, Landsman D: The Histone Database: a comprehensive resource for histones and histone fold-containing proteins. Proteins. 2006, 62: 838-842. 10.1002/prot.20814.
Luger K, Mader AW, Richmond RK, Sargent DF, Richmond TJ: Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature. 1997, 389: 251-260. 10.1038/38444.
Kornberg RD: Chromatin structure: a repeating unit of histones and DNA. Science. 1974, 184: 868-871. 10.1126/science.184.4139.868.
Gunjan A, Paik J, Verreault A: Regulation of histone synthesis and nucleosome assembly. Biochimie. 2005, 87: 625-635. 10.1016/j.biochi.2005.02.008.
Osley MA: The regulation of histone synthesis in the cell cycle. Annu Rev Biochem. 1991, 60: 827-861. 10.1146/annurev.bi.60.070191.004143.
Cho RJ, Campbell MJ, Winzeler EA, Steinmetz L, Conway A, Wodicka L, Wolfsberg TG, Gabrielian AE, Landsman D, Lockhart DJ, Davis RW: A genome-wide transcriptional analysis of the mitotic cell cycle. Mol Cell. 1998, 2: 65-73. 10.1016/S1097-2765(00)80114-8.
Cho RJ, Huang M, Campbell MJ, Dong H, Steinmetz L, Sapinoso L, Hampton G, Elledge SJ, Davis RW, Lockhart DJ: Transcriptional regulation and function during the human cell cycle. Nat Genet. 2001, 27: 48-54.
Oliva A, Rosebrock A, Ferrezuelo F, Pyne S, Chen H, Skiena S, Futcher B, Leatherwood J: The cell cycle-regulated genes of Schizosaccharomyces pombe. PLoS Biol. 2005, 3: e225-10.1371/journal.pbio.0030225.
Rustici G, Mata J, Kivinen K, Lio P, Penkett CJ, Burns G, Hayles J, Brazma A, Nurse P, Bahler J: Periodic gene expression program of the fission yeast cell cycle. Nat Genet. 2004, 36: 809-817. 10.1038/ng1377.
Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B: Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell. 1998, 9: 3273-3297.
Whitfield ML, Sherlock G, Saldanha AJ, Murray JI, Ball CA, Alexander KE, Matese JC, Perou CM, Hurt MM, Brown PO, Botstein D: Identification of genes periodically expressed in the human cell cycle and their expression in tumors. Mol Biol Cell. 2002, 13: 1977-2000. 10.1091/mbc.02-02-0030..
Birnbaum MJ, Wright KL, van Wijnen AJ, Ramsey-Ewing AL, Bourke MT, Last TJ, Aziz F, Frenkel B, Rao BR, Aronin N, et al: Functional role for Sp1 in the transcriptional amplification of a cell cycle regulated histone H4 gene. Biochemistry. 1995, 34: 7648-7658. 10.1021/bi00023a011.
Cross SL, Smith MM: Comparison of the structure and cell cycle expression of mRNAs encoded by two histone H3-H4 loci in Saccharomyces cerevisiae. Mol Cell Biol. 1988, 8: 945-954.
Eriksson PR, Mendiratta G, McLaughlin NB, Wolfsberg TG, Marino-Ramirez L, Pompa TA, Jainerin M, Landsman D, Shen CH, Clark DJ: Global regulation by the yeast Spt10 protein is mediated through chromatin structure and the histone upstream activating sequence elements. Mol Cell Biol. 2005, 25: 9127-9137. 10.1128/MCB.25.20.9127-9137.2005.
Fletcher C, Heintz N, Roeder RG: Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene. Cell. 1987, 51: 773-781. 10.1016/0092-8674(87)90100-0.
Matsumoto S, Yanagida M: Histone gene organization of fission yeast: a common upstream sequence. EMBO J. 1985, 4: 3531-3538.
Osley MA, Gould J, Kim S, Kane MY, Hereford L: Identification of sequences in a yeast histone promoter involved in periodic transcription. Cell. 1986, 45: 537-544. 10.1016/0092-8674(86)90285-0.
Oswald F, Dobner T, Lipp M: The E2F transcription factor activates a replication-dependent human H2A gene in early S phase of the cell cycle. Mol Cell Biol. 1996, 16: 1889-1895.
Sive HL, Heintz N, Roeder RG: Multiple sequence elements are required for maximal in vitro transcription of a human histone H2B gene. Mol Cell Biol. 1986, 6: 3329-3340.
van den Ent FM, van Wijnen AJ, Lian JB, Stein JL, Stein GS: Cell cycle controlled histone H1, H3, and H4 genes share unusual arrangements of recognition motifs for HiNF-D supporting a coordinate promoter binding mechanism. J Cell Physiol. 1994, 159: 515-530. 10.1002/jcp.1041590316.
Xie R, van Wijnen AJ, van Der Meijden C, Luong MX, Stein JL, Stein GS: The cell cycle control element of histone H4 gene transcription is maximally responsive to interferon regulatory factor pairs IRF-1/IRF-3 and IRF-1/IRF-7. J Biol Chem. 2001, 276: 18624-18632. 10.1074/jbc.M010391200.
Natsoulis G, Dollard C, Winston F, Boeke JD: The products of the SPT10 and SPT21 genes of Saccharomyces cerevisiae increase the amplitude of transcriptional regulation at a large number of unlinked loci. New Biol. 1991, 3: 1249-1259.
Natsoulis G, Winston F, Boeke JD: The SPT10 and SPT21 genes of Saccharomyces cerevisiae. Genetics. 1994, 136: 93-105.
Denis CL, Malvar T: The CCR4 gene from Saccharomyces cerevisiae is required for both nonfermentative and spt-mediated gene expression. Genetics. 1990, 124: 283-291.
Mendiratta G, Eriksson PR, Shen CH, Clark DJ: The DNA-binding domain of the yeast Spt10p activator includes a zinc finger that is homologous to foamy virus integrase. J Biol Chem. 2006, 281: 7040-7048. 10.1074/jbc.M511416200.
Chowdhary R, Ali RA, Albig W, Doenecke D, Bajic VB: Promoter modeling: the case study of mammalian histone promoters. Bioinformatics. 2005, 21: 2623-2628. 10.1093/bioinformatics/bti387.
Matys V, Kel-Margoulis OV, Fricke E, Liebich I, Land S, Barre-Dirrie A, Reuter I, Chekmenev D, Krull M, Hornischer K, et al: TRANSFAC and its module TRANSCompel: transcriptional gene regulation in eukaryotes. Nucleic Acids Res. 2006, 34: D108-D110. 10.1093/nar/gkj143.
Frith MC, Fu Y, Yu L, Chen JF, Hansen U, Weng Z: Detection of functional DNA motifs via statistical over-representation. Nucleic Acids Res. 2004, 32: 1372-1381. 10.1093/nar/gkh299.
Howe K, Bateman A, Durbin R: QuickTree: building huge Neighbour-Joining trees of protein sequences. Bioinformatics. 2002, 18: 1546-1547. 10.1093/bioinformatics/18.11.1546.
Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987, 4: 406-425.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.
Arents G, Moudrianakis EN: The histone fold: a ubiquitous architectural motif utilized in DNA compaction and protein dimerization. Proc Natl Acad Sci USA. 1995, 92: 11170-11174. 10.1073/pnas.92.24.11170.
Baxevanis AD, Arents G, Moudrianakis EN, Landsman D: A variety of DNA-binding and multimeric proteins contain the histone fold motif. Nucleic Acids Res. 1995, 23: 2685-2691. 10.1093/nar/23.14.2685.
Marino-Ramirez L, Kann MG, Shoemaker BA, Landsman D: Histone structure and nucleosome stability. Expert Rev Proteomics. 2005, 2: 719-729. 10.1586/147894126.96.36.1999.
Holm L, Sander C: Mapping the protein universe. Science. 1996, 273: 595-603. 10.1126/science.273.5275.595.
Eickbush TH, Moudrianakis EN: The histone core complex: an octamer assembled by two sets of protein-protein interactions. Biochemistry. 1978, 17: 4955-4964. 10.1021/bi00616a016.
Zhang Z, Gerstein M: Of mice and men: phylogenetic footprinting aids the discovery of regulatory elements. J Biol. 2003, 2: 11-10.1186/1475-4924-2-11.
Dominski Z, Marzluff WF: Formation of the 3' end of histone mRNA. Gene. 1999, 239: 1-14. 10.1016/S0378-1119(99)00367-4.
Bowman TL, Hurt MM: The coding sequences of mouse H2A and H3 histone genes contains a conserved seven nucleotide element that interacts with nuclear factors and is necessary for normal expression. Nucleic Acids Res. 1995, 23: 3083-3092. 10.1093/nar/23.16.3083.
Bowman TL, Kaludov NK, Klein M, Hurt MM: An H3 coding region regulatory element is common to all four nucleosomal classes of mouse histone-encoding genes. Gene. 1996, 176: 1-8. 10.1016/0378-1119(96)00198-9.
Eliassen KA, Baldwin A, Sikorski EM, Hurt MM: Role for a YY1-binding element in replication-dependent mouse histone gene expression. Mol Cell Biol. 1998, 18: 7106-7118.
Ma T, Van Tine BA, Wei Y, Garrett MD, Nelson D, Adams PD, Wang J, Qin J, Chow LT, Harper JW: Cell cycle-regulated phosphorylation of p220(NPAT) by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription. Genes Dev. 2000, 14: 2298-2313. 10.1101/gad.829500.
Wei Y, Jin J, Harper JW: The cyclin E/Cdk2 substrate and Cajal body component p220(NPAT) activates histone transcription through a novel LisH-like domain. Mol Cell Biol. 2003, 23: 3669-3680. 10.1128/MCB.23.10.3669-3680.2003.
Zhao J, Kennedy BK, Lawrence BD, Barbie DA, Matera AG, Fletcher JA, Harlow E: NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription. Genes Dev. 2000, 14: 2283-2297. 10.1101/gad.827700.
Tanay A, Regev A, Shamir R: Conservation and evolvability in regulatory networks: the evolution of ribosomal regulation in yeast. Proc Natl Acad Sci USA. 2005, 102: 7203-7208. 10.1073/pnas.0502521102.
Gasch AP, Moses AM, Chiang DY, Fraser HB, Berardini M, Eisen MB: Conservation and evolution of cis-regulatory systems in ascomycete fungi. PLoS Biol. 2004, 2: e398-10.1371/journal.pbio.0020398.
Luscombe NM, Babu MM, Yu H, Snyder M, Teichmann SA, Gerstein M: Genomic analysis of regulatory network dynamics reveals large topological changes. Nature. 2004, 431: 308-312. 10.1038/nature02782.
Madan Babu M, Teichmann SA, Aravind L: Evolutionary dynamics of prokaryotic transcriptional regulatory networks. J Mol Biol. 2006, 358: 614-633. 10.1016/j.jmb.2006.02.019.
Ihmels J, Bergmann S, Gerami-Nejad M, Yanai I, McClellan M, Berman J, Barkai N: Rewiring of the yeast transcriptional network through the evolution of motif usage. Science. 2005, 309: 938-940. 10.1126/science.1113833.
Gu Z, Nicolae D, Lu HH, Li WH: Rapid divergence in expression between duplicate genes inferred from microarray data. Trends Genet. 2002, 18: 609-613. 10.1016/S0168-9525(02)02837-8.
Makova KD, Li WH: Divergence in the spatial pattern of gene expression between human duplicate genes. Genome Res. 2003, 13: 1638-1645. 10.1101/gr.1133803.
Jensen LJ, Jensen TS, de Lichtenberg U, Brunak S, Bork P: Co-evolution of transcriptional and post-translational cell-cycle regulation. Nature. 2006, 443: 594-597.
NCBI Entrez Genome Project. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj]
Marzluff WF, Gongidi P, Woods KR, Jin J, Maltais LJ: The human and mouse replication-dependent histone genes. Genomics. 2002, 80: 487-498. 10.1016/S0888-7543(02)96850-3.
Lenhard B, Wasserman WW: TFBS: Computational framework for transcription factor binding site analysis. Bioinformatics. 2002, 18: 1135-1136. 10.1093/bioinformatics/18.8.1135.
Schneider TD, Stephens RM: Sequence logos: a new way to display consensus sequences. Nucleic Acids Res. 1990, 18: 6097-6100. 10.1093/nar/18.20.6097.
Crooks GE, Hon G, Chandonia JM, Brenner SE: WebLogo: a sequence logo generator. Genome Res. 2004, 14: 1188-1190. 10.1101/gr.849004.
Bateman A, Coin L, Durbin R, Finn RD, Hollich V, Griffiths-Jones S, Khanna A, Marshall M, Moxon S, Sonnhammer EL, et al: The Pfam protein families database. Nucleic Acids Res. 2004, 32: D138-D141. 10.1093/nar/gkh121.
Davey CA, Sargent DF, Luger K, Maeder AW, Richmond TJ: Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution. J Mol Biol. 2002, 319: 1097-1113. 10.1016/S0022-2836(02)00386-8.
Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE: The Protein Data Bank. Nucleic Acids Res. 2000, 28: 235-242. 10.1093/nar/28.1.235.
Dali Database. [http://ekhidna.biocenter.helsinki.fi/dali/start]
Kumar S, Tamura K, Nei M: MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform. 2004, 5: 150-163. 10.1093/bib/5.2.150.
Pavlidis P, Noble WS: Matrix2png: a utility for visualizing matrix data. Bioinformatics. 2003, 19: 295-296. 10.1093/bioinformatics/19.2.295.
Biowulf at the NIH. [http://biowulf.nih.gov]
Griffiths-Jones S, Moxon S, Marshall M, Khanna A, Eddy SR, Bateman A: Rfam: annotating non-coding RNAs in complete genomes. Nucleic Acids Res. 2005, 33: D121-D124. 10.1093/nar/gki081.
The authors would like to thank Alex Brick and Geoffrey Watson for their assistance in obtaining core histone intergenic regions during their internships at NCBI and Boris E Shakhnovich for helpful discussions. We are grateful to two anonymous reviewers for valuable comments and suggestions. This study utilized the high-performance computational capabilities of the Biowulf PC/Linux cluster at the National Institutes of Health, Bethesda, Maryland, USA . The authors wish to thank several anonymous reviewers for very helpful comments and suggestions. This research was supported by the Intramural Research Program of the NIH, NLM, and NCBI.
Electronic supplementary material
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Mariño-Ramírez, L., Jordan, I.K. & Landsman, D. Multiple independent evolutionary solutions to core histone gene regulation. Genome Biol 7, R122 (2006). https://doi.org/10.1186/gb-2006-7-12-r122
- Hide Markov Model
- Additional Data File
- Core Histone
- Histone Gene
- Histone Family