Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes
- Rammohan Narayanaswamy†1,
- Wei Niu†1,
- Alexander D Scouras†1,
- G Traver Hart1,
- Jonathan Davies1,
- Andrew D Ellington1,
- Vishwanath R Iyer1Email author and
- Edward M Marcotte1Email author
© Narayanaswamy et al.; licensee BioMed Central Ltd. 2006
Received: 25 July 2005
Accepted: 10 January 2006
Published: 31 January 2006
We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.
A major goal in functional genomics, proteomics, and systems biology is to define the biological functions of the genes encoded in each genome and to reconstruct the network of functional interactions that underlies normal and altered cellular and organismal biology . DNA microarrays, mass spectrometry, and protein-interaction screens have been powerful tools in this regard [2, 3], but it is important to employ diverse technologies addressing independent aspects of gene function in order to generate complementary datasets . In particular, spatial, temporal and phenotypic data provide important clues for understanding genetic circuitry.
In this paper, we describe a technology for measuring cell morphology and subcellular localization phenotypes, applied to a model system in which yeast change morphology in response to mating pheromone [5, 6]. Wild-type haploid yeast cells, on detecting pheromone of the opposite mating type via a cell surface receptor, heterotrimeric G protein, and mitogen-activated protein (MAP) kinase-mediated signal transduction cascade, arrest their cell cycles in G1 phase and grow in a polarized fashion towards the pheromone secreting cells, forming a characteristic cell shape termed a 'shmoo' . Several hundred genes change expression during this process . Shmoos of opposite mating type fuse, producing a diploid organism. The pheromone-response MAP kinase cascade is broadly conserved across eukaryotes, yet characterization of even this canonical signal transduction pathway is incomplete. Here, we describe the development of spotted cell microarrays and their application in defining genes controlling the response of yeast cells to mating pheromone.
High-density cell microarrays, with each spot containing cells from a distinct deletion strain and all of the strains represented on a single microscope slide, simplify automated image collection and minimize reagent use when probing the cells. With this approach, a single cellular feature can be examined in all approximately 4,800 genetic backgrounds, identifying genes contributing to that feature, associating genes with specific phenotypes, and providing information about spatial structures controlled by the genes. We have successfully created cell chips from 4,848 yeast deletion strains, automated collection of around 20,000 microscope images per cell chip, constructed the initial computational infrastructure to support the microscopy, and used cell chips to screen for genes affecting normal cellular morphology and for genes affecting the response of yeast to mating pheromone.
A high-throughput screen of yeast cellular morphology
Cells from each of the 4,848 distinct haploid yeast deletion strains, grown in rich media (YPD), were printed onto glass microscope slides coated with poly-L-lysine or concanavalin A (ConA) using a custom-built high-speed robotic arrayer that is normally used to manufacture DNA microarrays . Figure 1b shows an image of a cell microarray printed using this methodology. Each spot normally contains around 20-40 cells from a single deletion strain, as seen in Figure 1c using a standard microscope. Our preliminary data indicate that arrayed cells remain viable and physiologically normal after printing and washing, although cells are typically fixed for imaging purposes. A cell chip is analyzed using an automated fluorescence microscope to sequentially autofocus and image each spot.
Genes deleted from strains with an observed morphology defect were often functionally diverse. Nonetheless, certain general functions were enriched, which we evaluated by comparing the sets of strains exhibiting a given phenotype with the sets of strains previously known to exhibit characteristic cell morphologies  or with sets of genes associated with distinct Munich Information Center for Protein Sequences (MIPS) functions  or Gene Ontology  functions. Elongated strains were enriched (p < 0.01, as calculated using FunSpec ) for genes operating in nucleic acid metabolism, cell-cycle defects, transcription, and meiosis; large strains were enriched for transporter defects; round strains for cell wall, budding, cell polarity, and cell-differentiation genes; small strains for mitochondrial, carbohydrate metabolism, and phosphate-transport genes; and strains with polarized bud growth defects for budding, cell polarity, and filament-formation genes. Large and elongated strains significantly (p < 0.01) overlapped strains previously identified with these phenotypes during analysis of the homozygous diploid yeast deletion strains . Additional data files 2 and 3 summarize the morphological defects and functional enrichment, respectively.
Systematic identification of genes controlling mating-pheromone response
Having established the typical morphology of each haploid deletion strain, we examined the primary morphological differentiation pathway in budding yeast - the response of the cells to the mating pheromone alpha factor during sexual conjugation.
Although this pathway is well studied , it has yet to be analyzed to completion. We reasoned that additional genes affecting the pheromone-response pathway, either directly or indirectly, could be identified by examining shmoo phenotypes when the deletion collection was treated with alpha factor. We treated the entire mating type a haploid yeast deletion collection with alpha factor, then constructed and imaged spotted cell microarrays from the treated and fixed cells. Two graders manually examined the cell images for the absence of shmoos, grading the images on a numerical scale. Consistency between graders was high, and no systematic grading differences were apparent (see Additional data file 1). Defects in shmooing were found in 142 strains; these either formed no shmoos or formed barely detectable shmoos in the imaged fields of cells (Figure 2b and see Additional data files 2 and 4).
These 142 strains represent a mixture of genes participating in the pathway and false-positive results in the large-scale screen, primarily arising from stochastic sampling of cells from image fields with limited penetrance of shmoos and from ambiguity in identifying cells with mating projections. In practice, we explicitly included ambiguous cases for later retesting, thereby increasing the false-positive rate of the genome-wide screen but decreasing the false-negative rate (see Additional data file 1). We filtered this set for reproducible shmoo defects by manually retesting the 142 strains twice via alpha factor addition (to both mid-log and late-log phase cells) and microscopic imaging; 54 of the 142 strains showed consistent shmoo defects. Of these strains, ten were previously identified as diploid or MATalpha strains in the MATa haploid strain collection (A. Tong and C. Boone, personal communication), which correctly appear insensitive to alpha factor in this screen. Removing these strains (accounting for all diploid and MATalpha contaminants) and six strains whose deletions could not be confirmed by PCR or whose phenotype failed to reproduce in a reconstructed strain (see Additonal data file 1) leaves 38 MATa haploid strains reproducibly defective in shmoo formation. Note that deletion of one of these genes, GPA1 , is thought to be lethal except in the presence of additional pheromone pathway mutations , implying either strain-specific viability or additional suppressor mutations in the library strain.
Independent validation of mating-pheromone response genes
Figure 3 shows that 30 of the 38 reproducible shmoo-defective strains fail to arrest growth upon exposure to alpha factor to an extent comparable to the positive controls. Lack of growth arrest agreed well with reproducible shmoo defects. These strains were defective in both shmoo formation and growth arrest, implicating the deleted genes in the pathway. An additional four MATa haploid strains first identified as shmoo defective, but not among the 38 reproducibly shmoo-defective strains, also fail to arrest growth upon exposure to alpha factor, implicating the deleted genes in the pathway (see Additional data file 4). Enhanced shmooing strains arrest even more strongly and appear systematically hypersensitive to the pheromone (Figure 3). Thus, the extent of growth arrest in this assay correlates well with the penetrance of shmooing across the populations of cells as measured with the cell-chip assay.
Comparison with known pathway implicates new genes in pheromone response and shmoo formation
Beyond the known signal transduction pathway, 15 genes were found that fail to shmoo and fail to arrest growth upon exposure to alpha factor. Examples include genes with clear functions in polarized growth (BEM4 and BNI1 ), as well as regulatory functions (the histone deacetylase SDS3 and the ubiquitin protein ligase UBR2 ). We separately validated the BNI1 and UBR2 involvement by reconstructing and retesting the deletion strains. Other strains were PCR-confirmed for the identities of the deleted genes, but not reconstructed (see Additional data file 1), and thus should be validated by strain reconstruction before confirming the definite involvement of these genes in the pheromone-response pathway. There is a general implication of genes affecting membrane properties, including PDR17 , which controls phospholipid synthesis/transport  and LAS21 , which controls glycosylphosphatidylinositol-linked protein transport/remodeling . Several genes encoding plasma-membrane transporters are identified (QDR2 and DAL5 ), as well as a cell-wall biosynthetic enzyme (YEA4) and mannoprotein (TIR3). Loss of any of these genes disrupts pheromone response, possibly indicating membrane properties feeding back into control of mating response, consistent with the important role of plasma-membrane reorganization in shmooing .
Such comparisons with known and literature-associated pathway components, as well as strain reconstructions, allow us to estimate the false-positive rate of this screen. Of the 40 original genes (after removing MATalpha, diploids, and strains not verified by PCR), 15 are known pathway components, three (BEM4 , ISY1 , SDS3 ) can be reasonably implicated in polarized growth and pheromone response from literature, two (BNI1 , UBR2 ) were confirmed with reconstructed strains, and two were eliminated as false positives in reconstructed strains. Therefore, 20 of 40 genes were confirmed and two were false positives, placing the false positive rate at 2/22, or 9%. Not considering the three components implicated from the literature raises this to 2/19, or 11%. Nevertheless, as with any genome-wide screen we advise reconstruction of deletion strains before unequivocally concluding that these genes are implicated in the pheromone-response pathway.
Finally, we identified strains defective in only one of the two assayed phenotypes, implicating the genes in downstream pathways. The set of strains that fail to arrest yet shmoo properly (termed AD for arrest defective) was functionally diverse as well as small (in part because only around 8% of the deletion collection was tested for growth arrest - we expect more such mutants given a complete screen for growth arrest). These strains were deleted for FMP35, RPL37B , YHL042W , YDR360W , YGL214W , PUB1 , PMT2 , TRX2 , SFK1 , MUP3 , SPL2 , and STM1. Conversely, eight genes were identified arresting normally yet failing to shmoo (termed SD for shmoo defective). Interestingly, six of these (VPS8 , VPS21 , VPS22 , VPS23 , VPS28 , VPS36 ) are involved in vacuolar protein sorting, with all but VPS8 and VPS21 specific to class E sorting and resulting in inefficient transport out of the endosome , suggesting a critical role of this system in shmoo formation (that is, downstream of pheromone signaling), possibly related to plasma-membrane reorganization [37, 39]. The remaining two proteins are involved in polarized growth (ECM33 ) and transcriptional regulation (the histone acetylase EAF3 ).
We attempted to connect the 15 putative pheromone-response implicated genes (the ASD set) to the known pathway (the core set) using available functional genomics data by searching for the shortest pathways through protein interaction and mRNA coexpression networks  that connected the new genes to the core set. Nine of the new genes could be reasonably connected to the core set by two interactions or fewer (Figure 5), indicating that these genes may have direct, rather than indirect, roles in the pheromone response pathway. As connecting 9 of 15 genes to the core is no more than expected by random trials, these linkages serve only as hypotheses to provide a starting point for experiments validating the associations.
One gene connected in this manner is SDS3 , a component of the Rpd3/Sin3 histone deacetylase complex implicated in gene silencing , and it is likely that the implication of SDS3 in the pheromone response pathway probably stems from the action of this complex on the silencing of mating loci. Likewise, another gene implicated in the screen, the ubiquitin protein ligase UBR2 , is an interaction partner of DOT1 , a participant in Sir-mediated gene silencing , and thus a reasonable inference is that deletion of UBR2 may also influence silencing. Another gene from the screen, ISY1 , is pleiotropic but connected to control the cell cycle, participating in mRNA splicing and the spindle checkpoint . ISY1 exhibits some connection to polarized growth: homozygous diploid deletions of ISY1 exhibit abnormal axial budding . Although MRPL28 can be connected the core network in this manner, its shmoo defect might also arise by a disruption in the deletion strain of the proper functioning of the adjacent MFA1 alpha factor mating-pheromone gene.
Cell morphology phenotypes are rich in information, and although we have focused on strains exhibiting a failure to shmoo, additional strains were identified with morphological defects in the mating projections, such as shown for the kel1 ΔKanMX4 strain of Figure 2b. We flagged a total of 29 strains producing shmoos of aberrant morphology. These strains, listed in Additional data file 5, are deleted for genes involved in a statistically significant (p < 0.01 ) fashion in mating, especially for genes of polarized growth (CDC10 , KEL1 , and BUD19 ), but also for genes of transcriptional and translational regulation, including components of transcription and chromatin remodeling (SNF6 , SPT3 , SPT10 , HTL1 , and SIN4 ), translational regulation (CBP6 , ASC1 , and SRO9 ), and rRNA processing/ribosome biogenesis (NSR1 , RPP1A , RPL31A , RPS16B , and RAI1 ). There is also some interplay between cell morphology and pheromone response phenotypes - for example, the mrpl28 ΔKanMX4 strain exhibits a large cell phenotype until alpha factor is added, whereupon the cell size defect is corrected, although the cells fail to shmoo (see Additional data file 1).
Interestingly, we also find the extent of alpha factor-induced growth arrest appears largely uncorrelated with the change in expression of the corresponding genes following alpha factor treatment in wild-type cells , even for known genes in the core pathway (see Additional data file 1). Instead, the known pathway genes fall into two categories: those whose deletion strains show strong alpha factor-induced growth arrest or those that fail to arrest. The former category is exclusively composed of inhibitors of pheromone-response components. The majority of known pathway genes do not change expression following alpha factor treatment , nor do the majority of new genes implicated in the pathway by the combined cell chip/growth inhibition assay. Therefore, the cell chip-based screen complements the information available from DNA microarrays.
In conclusion, we describe a new genomic-scale technology for microscopy on genetically distinct cells, applied here to measuring the cell morphologies of yeast in the haploid deletion strain collection and to the mapping of genes participating in the response of yeast cells to mating pheromone. Although this paper focuses on cell morphology, cell chips have utility beyond this and can in principle be extended to any organism or cell type for which defined libraries of cells can be arrayed, such as other easily manipulated organisms, banks of bacteria, and deletion libraries for other microorganisms. We expect that diverse collections of strains can be arrayed, such as yeast strains in which proteins are tagged with green fluorescent protein . Just as it proved possible to identify pathways modulated by alpha factor, it should be possible to quickly identify mutants and pathways differentially affected by drugs. A major advantage of the cell chips is the minimal use of expensive reagents on the chips, achieved by limiting the use of antibodies and dyes to single microscope slides, as compared to the approximately 50 96-well plates required to image the complete deletion collection.
The key principle distinguishing cell chips from other approaches (such as immunoassays in 96-well plates) is, however, the separation of cell growth from imaging. Thus, we anticipate the strongest advantage of cell chips will be their use for analyzing the localization of proteins or RNAs by high-throughput in situ hybridization and antibody-based immunoassays. Consider the case of printing multiple identical cell chips, but probing each with a different set of dyes or antibodies. Each slide then becomes a unique assay for the dye or antibody target across the set of genetically distinct strains. In this mode, cells from the deletion strain collection are fixed, spheroplasted, and spotted onto microarrays, effectively separating the growth of the cells from the imaging process (a strategy difficult to achieve with plate assays). Around 200 cell chips can be made in a single printing session; each serves as a separate imaging assay when probed with an antibody to a distinct target, revealing the change in localization and expression of that target across the approximately 4,800 genetic backgrounds. The resulting images would indicate synthetic genetic interactions between the probe targets and the deleted genes, and the act of imaging becomes a scaleable, easily replicated assay on standardized cell chips for the high-throughput generation of synthetic interactions. Combining cell-chip throughput with automated image processing [12, 46, 47] should provide quantitative strain- and gene-specific data. Data from such experiments will generate functional and statistical connectivities between genes , ultimately leading to comprehensive network analyses of genes .
Materials and methods
Cell microarray construction and imaging
All methods are described in full in Additional data file 1. In brief, cell microarrays were constructed by contact deposition of suspensions of yeast cells from the arrayed collection of S. cerevisiae haploid deletion strains (BY4741 genetic background; MAT a his3 Δleu2 Δmet15 Δura3 Δ) onto Con A  or poly-L-lysine-coated glass slides using a custom-built DNA microarray printing robot . In about 12 h, more than 100 slides can be printed, each containing the entire deletion collection as well as the isogenic wild-type parent strain as a control. Cell arrays may be used for imaging immediately after printing or stored at 4°C or -80°C, provided that the cells are printed with glycerol. Centrifugation enhances adherence of cells to the slide, permitting washing before staining and imaging. Cell images were collected via automated microscopy, using a Nikon E800 microscope with computer-controlled X-Y stage and piezoelectric-positioned objective, by scanning to the position of each spot, autofocusing, and capturing the image with a Coolsnap CCD camera (Photometrics, Tucson, USA). Images were stored in a custom cell microarray image database (Cellma)  for manual examination or automated image analysis. Using Perl scripts and custom MetaMorph (Universal Imaging Corporation, Sunnyvale, USA) journals, a full set of approximately 5,000 images can be collected from a slide in bright-field mode in less than 4 hours or for fluorescent images in around 10 h. Control cell chips, grading schemes, and morphology analysis details are described in the Additional data file 1.
High-throughput screen for strains unresponsive to alpha factor
To examine cell morphology after stimulation with alpha factor, each yeast deletion strain was subcultured into fresh YPD medium in 96-well Costar tissue culture plates (Corning, Corning, USA), grown for 36 hours at 30°C, centrifuged, and washed with YPD, pH 3.5, to inactivate the Bar1p protease . Alpha factor (350 μg/ml) was added to each sample well, a concentration measured by titration (as in ) to induce shmoo formation in around one half of the cells in the majority of deletion strains (see Additional data file 1). Cells were incubated for 4 hours at 30°C, fixed with 3.7% formaldehyde for 1 hour at room temperature, washed with YPD containing 17% (w/v) glycerol, supplemented with 20 mM CaCl2, 20 mM MnSO4, then spotted onto Con A-coated glass slides. Slides were stained with DAPI, imaged by automated microscopy, and manually scored by two independent graders for extent of shmooing.
Assay of alpha-factor-induced growth arrest
Four hundred and twenty-six selected deletion strains were grown overnight in YPD, centrifuged, and washed with YPD, pH 3.5 . The cultures were split into replicate 96-well plates of YPD, with and without alpha factor at a final concentration of 25 μg/ml, maintaining cells at an optical density at 600 nm (OD600) of around 0.2-0.5 . Plates were incubated at 30°C for 10 h, recording OD600 hourly from each strain. The slope of each growth curve was calculated from a plot of log(OD600) versus time. The effect of alpha factor on the strains was obtained as the ratio of the slope from the untreated sample to that of the alpha-factor-treated sample. Average ratios were calculated from two or three independent assays.
Additional data files
The following additional data are available online with this paper. A detailed description of methods for constructing, imaging, and evaluating spotted cell microarrays is included as Additional data file 1. Data relevant to the measured cell morphologies (Additional data file 2), computational analysis of enriched functions (Additional data file 3), pheromone growth-arrest phenotypes (Additional data file 4), and lists of implicated genes (Additional data file 5) are available. All spotted cell microarray image data and experimental protocols are available from the Cellma cell microarray database .
We thank Zack Simpson for aid in image storage/analysis and Kerri Keiger for help with array printing. This work was supported by grants from the Welch and Packard Foundations (E.M.M.), the Beckman Foundation/MURI site grant (A.D.E., E.M.M.), and the National Science Foundation and National Institutes of Health.
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