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Open Access

Erratum to: A DNA microarray survey of gene expression in normal human tissues

  • Radha Shyamsundar1, 2,
  • Young H Kim1,
  • John P Higgins1,
  • Kelli Montgomery1,
  • Michelle Jorden1,
  • Anand Sethuraman3,
  • Matt van de Rijn1, 2,
  • David Botstein3, 5,
  • Patrick O Brown2, 4Email author and
  • Jonathan R Pollack1Email author
Genome Biology20056:404

Published: 19 August 2005

The original article was published in Genome Biology 2005 6:R22

We wish to report two corrections to our study [1], neither of which alters the interpretation of the data or the conclusions drawn. First, we have discovered that the data file from a microarray hybridization (prostate RNA versus normal genomic DNA) used to derive the plot in Figure 4a became corrupted during data processing. The corrected plot (Figure 4a) displays a stronger correlation between directly and indirectly estimated transcript levels, indicating even better performance of our method of estimating transcript abundance. The corrected data file has been deposited to the Stanford Microarray Database (SMD) and Gene Expression Omnibus (GEO) repositories. Second, we have identified a 'frame-shift' in the Additional data file 2 (Sheet 5) data set; the corrected data file has been deposited to the supplemental site.
Figure 4

Estimating relative transcript abundance. (a) Comparison of transcript levels estimated either directly by hybridization of prostate sample mRNA versus normal female genomic DNA, or indirectly by multiplying the ratio of prostate sample mRNA versus common reference mRNA by the ratio of common reference mRNA versus normal female genomic DNA. The correlation value (R) is indicated. (b) Prostate-specific gene expression cluster, extracted from the hierarchical cluster shown in Figure 1a, displayed as mean-centered relative gene expression (ratio-fold change scale indicated). (c) The same gene expression feature as in (b), now displayed as transcript abundance (relative to the average transcript level for all expressed genes), calculated indirectly using the common reference mRNA versus normal female genomic DNA hybridization data.

Additional data files

Additional data file 2 contains a corrected list of the variably expressed genes.



Authors’ Affiliations

Department of Pathology, Stanford University
Department of Biochemistry, Stanford University
Department of Genetics, Stanford University
Howard Hughes Medical Institute, Stanford University
Lewis-Sigler Institute for Integrative Genomics, Princeton University


  1. Shyamsundar R, Kim YH, Higgins JP, Montgomery K, Jorden M, Sethuraman A, van de Rijn M, Botstein D, Brown PO, Pollack JR: A DNA microarray survey of gene expression in normal human tissues. Genome Biol. 2005, 6: R22-10.1186/gb-2005-6-3-r22.PubMedPubMed CentralView ArticleGoogle Scholar


© BioMed Central Ltd 2005