Drosophila as an emerging model to study metastasis
Genome Biology volume 5, Article number: 216 (2004)
Metastasis is the primary cause of human cancer-related deaths. Two recent studies describe a system for testing how multiple genetic events synergize to promote neoplastic growth and metastasis in Drosophila, paving the way for systematic approaches to understanding metastasis using the powerful tools of Drosophila genetics.
Modeling metastasis in Drosophila
Cancer can be thought of as a genetic disease caused by the accumulation of multiple genetic or epigenetic lesions in tumor-suppressor genes and oncogenes; the resulting processes culminate in cell proliferation, survival and metastasis (colonization of distant sites by tumor cells) . But depending on the sequence in which cells accumulate genetic lesions, the ensuing tumor progression and metastasis are highly variable, even among tumors of the same type . Furthermore, only a small fraction of tumor cells achieve metastatic competence, suggesting that the combination of required events is acquired only rarely. Our current understanding of the molecular processes leading to metastasis is largely derived from studies of cancer cell lines in vitro, xenografts of human tumors, and a limited number of transgenic or knockout mouse models [2, 3]. These systems may not reflect the normal processes involved in tumorigenesis or, as in case of murine models, may be too cumbersome to be used for analyzing multiple genetic interactions. A major challenge in cancer research is, therefore, to develop model systems that allow efficient analysis of the combinations of genetic events that trigger the initiation of metastasis during tumor development in vivo.
In order to study metastasis in vivo, it would be ideal to use a model organism in which multiple genetic lesions could be introduced in a controlled way into subpopulations of cells, which can then be analyzed for proliferation, cell migration, invasion and metastasis. The fruit fly Drosophila researchers have an arsenal of sophisticated genetic manipulation techniques that have proven invaluable for dissecting the signaling pathways that affect cell specification, differentiation and growth [4–7]. Furthermore, Drosophila is highly amenable to genetic screens for previously unidentified components of signaling pathways. Knowledge gained using Drosophila is often applicable to human biology, because pathways controlling virtually all cell-biological processes are highly conserved between flies and humans . Two papers, by the Richardson and Xu groups [9, 10], now demonstrate the use of Drosophila to investigate the combinations of genetic aberrations that trigger metastasis.
The key to studying how combinations of genetic alterations cause metastasis in Drosophila was the generation of clones of cells that carried multiple genetic manipulations such as mutations in tumor-suppressor genes and overexpression of oncogenes [9, 10]. In addition, the manipulated cells also expressed green fluorescent protein (GFP), which allowed their detection and analysis of their proliferation, migration and invasive behavior in vivo in the context of unmanipulated cells. Such GFP-marked clones were generated by using a powerful genetic technique called 'mosaic analysis with a repressible cell marker' (MARCM), which produces clones of cells that are mutant for a gene of interest and that simultaneously overexpress one or more genes, such as oncogenes and GFP . Using MARCM clones, the Richardson  and Xu  groups found that a specific combination of defects, namely disruption of apical-basal cell polarity together with the expression of an activated version of the oncogene Ras, results in excessive cell proliferation and metastasis.
Genetic lesions that synergize to give metastasis
Both groups [9, 10] found that overexpression of oncogenic Ras (the RasV12variant) in clones mutant for the tumor-suppressor gene scribble (scrib) resulted in excessive proliferation and metastasis [9, 10]. Scrib is an adaptor protein containing multiple PDZ domains (which enable binding to a variety of other proteins); it is localized to basolateral membranes of epithelial cells . The Xu group  tested whether mutations in tumor-suppressor genes - such as scrib or warts, which encodes a protein kinase of the DMPK family - synergize with oncogenic Ras to induce metastasis, whereas the Richardson group  examined the effects of activation of various signaling pathways on the growth and survival of scrib mutant cells. To do so, both groups induced MARCM clones specifically in the cephalic complexes of Drosophila larvae (the eye imaginal discs, which are epithelial sacs that give rise to adult eyes, and the optic lobes of the brain) using a tissue-specific driver containing the promoter of the eyeless transcription-factor gene coupled to the Flp recombinase gene (eyFLP). eyFlp induces mitotic recombination, producing clones of homozygous mutant cells . Mutant cell clones were then analyzed for cell proliferation and for invasion of distant larval tissues. RasV12scrib- mutant cells overproliferate and form secondary tumors in the ventral nerve cord, imaginal tissues and tracheal branches in the mutant animals [9, 10].
This effect is striking because neither loss of scrib nor overexpression of RasV12individually causes invasive behavior [9, 10, 14]. Loss of scrib in homozygous mutant animals results in loss of epithelial (apical-basal) polarity and causes neoplastic overgrowths of imaginal tissues [12, 15]. Overexpression of oncogenic Ras alone results in hyperplastic overgrowth of imaginal tissues . Thus, loss of scrib or activation of oncogenic Ras can induce only excessive proliferation, but not metastasis, in imaginal tissues. In addition, deletion of warts does not phenocopy scrib deletion in cells overexpressing RasV12. The synthetic effect of loss of scrib function with Ras activation thus represents a specific combination of genetic changes that together induce metastasis.
Pagliarini and Xu  asked whether the metastasis observed in RasV12scrib- cells exhibits features of human metastatic tumors. Normally, the integrity of epithelial tissues is maintained by adhesive cell-cell interactions . Defects in tissue integrity allow tumor cells to acquire motility and become metastatic; as part of this process, mammalian metastatic tumors often downregulate the cell-adhesion molecule E-cadherin . Similar effects occur in Drosophila, in which E-cadherin expression is downregulated in RasV12scrib- tumors . Downregulation of E-cadherin is important for the metastatic behavior of Drosophila tumors, because overexpression of E-cadherin suppresses metastasis in RasV12scrib- cells. Loss of E-cadherin does not mimic the loss of scrib, however, because metastasis is not observed upon activation of RasV12 in E-cadherin mutants. The loss of scrib must therefore have effects in addition to loss of E-cadherin to promote metastasis in cells overexpressing oncogenic Ras. Another ability that is acquired by mammalian tumors is degradation of basement membranes. Using an antibody to detect laminin and a collagen IV-GFP fusion protein to detect basement membranes, Pagliarini and Xu  found that metastatic RasV12scrib- tumor cells degrade basement membranes in Drosophila. By the criteria of loss of cell adhesion and degradation of basement membranes, therefore, they concluded that these metastatic tumors mimic critical features of metastatic mammalian tumors .
The scrib gene has a pivotal role in establishing cell polarity in association with two other Drosophila neoplastic tumor-suppressor genes, lethal(2) giant larvae and discs large [15, 18]. The lethal(2) giant larvae gene encodes a protein that contains WD4O repeats (predicted to be involved in protein-protein interactions) and discs large encodes a member of the membrane-associated guanylate kinase (MAGUK) family that contains multiple PDZ domains known to be involved in targeting signaling proteins to the cell membrane. Pagliarini and Xu  therefore asked whether the metastatic behavior of RasV12scrib- cells is due to loss of cell polarity. They found that lethal(2) giant larvae, discs large and other cell-polarity genes such as bazooka and Stardust, both of which encode multi-PDZ domain-containing proteins, as well as cdc42, encoding a small GTPase, which individually do not affect growth, also impart metastatic potential to cells overexpressing RasV12. Thus, loss of cell polarity in general, not just through loss of scrib, collaborates with oncogenic Ras to promote metastasis [9, 10]. Defects in cell polarity result in an epithelial-to-mesenchymal transition, disruption of intercellular junctions and loss of cell adhesion; these defects may be important for the progression of cells from neoplastic (tumorigenic) to metastatic behavior.
A new role for Ras in metastasis
Why do mutations in scrib synergize so strongly with activation of Ras? As in vertebrates, Ras in Drosophila promotes cell survival, growth and proliferation . If the effects of Ras function through these processes, then overexpression or overactivation of the downstream effectors involved in cell survival, growth and proliferation in scrib mutant cells should mimic the effects of RasV12. Overexpression of an activated version of the Ras effector protein Raf mimics the effects of Ras overexpression in scrib mutant cells, indicating that Ras mediates its oncogenic effects by signaling through Raf . Clones of cells mutant for scrib alone survive poorly [9, 10], and Ras may thus collaborate with scrib to promote their survival. The poor survival of scrib mutant cells depends on the presence of neighboring wild-type cells, and scrib mutant cells survive if the entire tissue is mutant . It seems, therefore, that scrib mutant cells are not destined to die because of intrinsic defects in their cell physiology, but rather they are actively killed by a process requiring interactions between scrib mutant and neighboring wild-type cells, a process referred to as cell competition . The nature of the death-inducing signal is not known, but the elimination of scrib mutant cells requires activation of signaling through the Jun N-terminal kinase (JNK) pathway, as is the case for other manipulations that trigger cell competition [21–23]. Thus, blocking JNK signaling in scrib mutant cell clones rescues them from apoptosis . These cells still do not metastasize, however; and RasV12must therefore have effects, in addition to promoting survival of scrib mutant cells, that impart metastatic potential.
To test whether promotion of cell proliferation and cell growth can together mimic the effects of activated Ras, the two groups overexpressed the transcription factor E2F together with its binding partner Dp, to promote cell proliferation, and the phosphatidylinositol 3-kinase Akt or the transcription factor Myc, to promote cell growth, in scrib mutant cells. Overexpression of any of these effectors alone did not mimic the effects of RasV12, however. Moreover, coexpression of E2F, Akt and the apoptosis inhibitor p35 together also did not mimic the effects of RasV12. Thus, the combination of blocking cell death (with p35), promoting cell proliferation (E2F/Dp) and promoting cell growth (Akt) does not phenocopy the effects of Ras activation [9, 10]. Ras therefore mediates its oncogenic effects through other (unknown) downstream effectors [9, 10].
The Richardson group  tested whether other signaling pathways can cause scrib mutant cells to metastasize. They found that activation of the signaling pathways initiated by any of the extracellular ligands Decapentaplegic, Hedgehog and Wingless did not show a strong effect on scrib mutant cells . In contrast, the activation of the Notch receptor signaling pathway had similar effects on scrib mutant cells to the coexpression of oncogenic Ras, but it remains to be determined whether Notch signals through Ras or independently .
Advantages of Drosophila
The MARCM system can mimic the clonal nature of mammalian cancer, because it allows simultaneous manipulation of multiple genes in small populations of cells. The metastasis models described in the two recent papers [9, 10] can now be used to perform systematic screens for secondary mutations that modify (enhance or suppress) the metastatic phenotypes. It is therefore now possible to do genome-wide screens for mutations that promote growth and metastasis in combination with other oncogenes and tumor-suppressor genes. Even though not all aspects of human cancer and metastasis may have parallels in flies, we can expect many exciting new discoveries to be made using flies as a model to study metastasis.
Hanahan D, Weinberg RA: The hallmarks of cancer.Cell 2000, 100:57–70.
Van Dyke T, Jacks T: Cancer modeling in the modern era: progress and challenges.Cell 2002, 108:135–144.
Balmain A: Cancer as a complex genetic trait: tumor susceptibility in humans and mouse models.Cell 2002, 108:145–152.
St Johnston D: The art and design of genetic screens:Drosophila melanogaster.Nat Rev Genet 2002, 3:176–188.
Blair SS: Genetic mosaic techniques for studyingDrosophiladevelopment.Development 2003, 130:5065–5072.
Kornberg TB, Krasnow MA: TheDrosophilagenome sequence: implications for biology and medicine.Science 2000, 287:2218–2220.
Rubin GM, Lewis EB: A brief history ofDrosophila's contributions to genome research.Science 2000, 287:2216–2218.
Edwards PA: The impact of developmental biology on cancer research: an overview.Cancer Metastasis Rev 1999, 18:175–180.
Brumby AM, Richardson HE: scribblemutants cooperate with oncogenic Ras or Notch to cause neoplastic overgrowth inDrosophila.EMBO J 2003, 22:5769–5779.
Pagliarini RA, Xu T: A genetic screen inDrosophilafor metastatic behavior.Science 2003, 302:1227–1231.
Lee T, Luo L: Mosaic analysis with a repressible neurotechnique cell marker for studies of gene function in neuronal morphogenesis.Neuron 1999, 22:451–461.
Bilder D, Perrimon N: Localization of apical epithelial determinants by the basolateral PDZ protein Scribble.Nature 2000, 403:676–680.
Xu T, Rubin GM: Analysis of genetic mosaics in developing and adultDrosophilatissues.Development 1993, 117:1223–1237.
Karim FD, Rubin GM: Ectopic expression of activated Ras1 induces hyperplastic growth and increased cell death inDrosophilaimaginal tissues.Development 1998, 125:1–9.
Humbert P, Russell S, Richardson H: Dlg, Scribble and Lgl in cell polarity, cell proliferation and cancer.BioEssays 2003, 25:542–553.
Gibson MC, Perrimon N: Apicobasal polarization: epithelial form and function.Curr Opin Cell Biol 2003, 15:747–752.
Christofori G: Changing neighbours, changing behavior: cell adhesion molecule-mediated signalling during tumor progression.EMBO J 2003, 22:2318–2323.
Wodarz A: Tumor suppressors: linking cell polarity and growth control.Curr Biol 2000, 10:R624-R626.
Shields JM, Pruitt K, McFall A, Shaub A, Der CJ: Understanding Ras: 'it ain't over 'til it's over'.Trends Cell Biol 2000, 10:147–154.
Simpson P: Parameters of cell competition in the compartments of the wing disc ofDrosophila.Dev Biol 1979, 69:182–193.
Adachi-Yamada T, Fujimura-Kamada K, Nishida Y, Matsumoto K: Distortion of proximodistal information causes JNK-dependent apoptosis inDrosophilawing.Nature 1999, 400:166–169.
Adachi-Yamada T, O'Connor MB: Morphogenetic apoptosis: a mechanism for correcting discontinuities in morphogen gradients.Dev Biol 2002, 251:74–90.
Moreno E, Basler K, Morata G: Cells compete for decapentaplegic survival factor to prevent apoptosis inDrosophilawing development.Nature 2002, 416:755–759.
We thank Randy L. Johnson, Richard Behringer, Gordon Mills, Kwang Wook Choi, Amit Singh, Riitta Nob, Astrid Eder, Robin P. Heisinger, Karthik Pappu, and members of the Haider laboratory for their helpful suggestions.
About this article
Cite this article
Kango-Singh, M., Halder, G. Drosophila as an emerging model to study metastasis. Genome Biol 5, 216 (2004). https://doi.org/10.1186/gb-2004-5-4-216
- Cell Competition
- Giant Larva
- Imaginal Tissue
- Notch Receptor Signaling
- Homozygous Mutant Animal