- Open Access
Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation
© Sempere et al. 2004
- Received: 17 October 2003
- Accepted: 8 January 2004
- Published: 16 February 2004
The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18 to 25 nucleotides) with probable roles in the regulation of gene expression. In Caenorhabditis elegans, lin-4 and let-7 miRNAs control the timing of fate specification of neuronal and hypodermal cells during larval development. lin-4, let-7 and other miRNA genes are conserved in mammals, and their potential functions in mammalian development are under active study.
In order to identify mammalian miRNAs that might function in development, we characterized the expression of 119 previously reported miRNAs in adult organs from mouse and human using northern blot analysis. Of these, 30 miRNAs were specifically expressed or greatly enriched in a particular organ (brain, lung, liver or skeletal muscle). This suggests organ- or tissue-specific functions for miRNAs. To test if any of the 66 brain-expressed miRNAs were present in neurons, embryonal carcinoma cells were treated with all-trans-retinoic acid to promote neuronal differentiation. A total of 19 brain-expressed miRNAs (including lin-4 and let-7 orthologs) were coordinately upregulated in both human and mouse embryonal carcinoma cells during neuronal differentiation. The mammalian ortholog of C. elegans lin-28, which is downregulated by lin-4 in worms via 3' untranslated region binding, was also repressed during neuronal differentiation of mammalian embryonal carcinoma cells. Mammalian lin-28 messenger RNAs contain conserved predicted binding sites in their 3' untranslated regions for neuron-expressed miR-125b (a lin-4 ortholog), let-7a, and miR-218.
The identification of a subset of brain-expressed miRNAs whose expression behavior is conserved in both mouse and human differentiating neurons implicates these miRNAs in mammalian neuronal development or function.
- Chronic Lymphocytic Leukemia
- Neuronal Differentiation
- Additional Data File
- miRNA Gene
- Northern Analysis
The microRNAs (miRNAs) are an extensive class of small non-coding RNAs with mature transcripts of 18 to 25 nucleotides [1–12]. The enzymatic machinery involved in the biogenesis of miRNAs in plants and animals is well conserved. RNAse type III-like Dicer, together with Argonaute proteins, cleaves a miRNA hairpin precursor (70 to 75 nucleotides) to yield a stable, ~22 nucleotides miRNA from one arm of the hairpin . Some miRNA genes are often organized into clusters [1–3, 14, 15], and in some cases clustered miRNAs are co-expressed [14, 15]. Coordinate transcription is the first of multiple levels for regulation of miRNA expression, including miRNA maturation, transport, and stability [15–17]. This gives plants and animals a great flexibility to modulate the levels of miRNAs in different cell types and tissues and at different stages of their development.
The biological functions of a handful of miRNAs have been identified in plants and animals [13, 18]. The most apparent difference between plant and animal miRNAs is the way they interact with target genes. Animal miRNAs are generally thought to recognize their messenger RNA (mRNA) targets by incomplete base-pairing, leading to translational inhibition of the target. Plant miRNAs apparently also inhibit translation , but many plant miRNAs are precisely complementary to their targets, and in these cases they act as guides to trigger transcript degradation through RNA interference (RNAi) [20–22]. In Caenorhabditis elegans, upregulation of lin-4 and let-7 RNAs during the first and fourth larval stage, respectively, triggers an ordered downregulation of target genes that is required for the sequential expression of stage-specific developmental programs [23–26]. lin-4 RNA binds to the 3' untranslated region (UTR) of lin-14 and lin-28 mRNAs and inhibits their translation [27, 28]. Similarly, let-7 RNA binds to 3' UTR of lin-41 and lin-57 mRNAs and inhibits their expression at a post-transcriptional level [29–31]. In Drosophila melanogaster, the mRNAs of the pro-apoptotic gene hid and the apoptotic effector gene drice possess binding sites for bantam and miR-14, respectively [32, 33]. The phenotypes of bantam and mir-14 loss-of-function suggest that these miRNAs regulate apoptosis by inhibiting translation of these apoptosis-promoting genes [32, 33].
In mammals, certain miRNAs have been implicated in the maintenance of pluripotent cell state during early embryogenesis , and roles have been suggested for other miRNAs in tissue-specific or organ-specific development . However, hundreds of miRNAs have been cloned from mouse and human organs and cell lines [1–3, 9, 10], and numerous additional miRNAs have been predicted with computational algorithms . To begin to explore the possible roles for these numerous miRNAs in mammalian development, we used northern analysis to characterize the expression of 119 previously reported miRNAs in adult organs from mouse and human. Of these, 30 miRNAs were specifically expressed or greatly enriched in a particular organ, suggesting an organ- or tissue-specific function. A small subset of brain-expressed miRNAs was induced in mouse and human embryonal carcinoma (EC) cells treated with all-trans-retinoic acid (RA). This induction coincided closely with the time of terminal neuronal differentiation. The evolutionary conserved expression of these miRNAs suggests that they may play a role in the specification and/or progression of neuronal lineage. The orthologous protein of C. elegans LIN-28 was repressed in these EC cells treated with RA. In addition, mammalian Lin-28 mRNAs contain conserved predicted binding sites in their 3' UTRs for neuron-expressed miR-125b (a lin-4 ortholog), let-7a, and miR-218. Thus, Lin-28 may also be subjected to miRNA regulation in mammalian cells.
Diverse miRNA expression patterns in mammalian organs
To identify additional classes of miRNAs with similar expression profiles, all 119 miRNAs were hierarchically clustered on the basis of their expression profiles in our experiments, applying Pearson's distribution. From this analysis, at least six major groups of miRNAs with similar expression profiles were discerned (Figure 2c). A group of six miRNAs was expressed primarily in mouse spleen (miR-127, -142-a, -142-s, -151, -189, -212). A group of five miRNAs was expressed in mouse and human liver (miR-122a, -152, -194, -199, -215) with some scattered expression in other organs including lung and kidney. A group of seven miRNAs was expressed in mouse lung and kidney (miR-18, -20, -24, -32, -141, -193, -200b). Together, the last two groups might reflect a role of miRNAs in an epithelial cell type since liver, lung, and kidney are organs containing epithelial tissues. A group of 17 miRNAs was expressed in mouse and human brain (miR-7, -9, -9*, -124a, -124b, -125a, -125b, -128, -132, -135, -137, -139, -153, -149, -183, -190, -219) with scattered expression in other organs. This group included all seven brain-specific and seven brain-enriched miRNAs described above. The brain is a complex organ with several cell types, among which neurons and glial cells are predominant. Future work will determine the specific neuronal cells in which these miRNAs are expressed. A group of six miRNAs was expressed in mouse and human skeletal muscle and heart: miR-1b, -1d, -133 and -206 had elevated expression in heart and skeletal muscle with low expression in other organs and miR-143 and -208 were almost exclusively detected in heart and skeletal muscle. Skeletal muscle and heart derive from the mesodermal embryonic layer and possess similar striated muscle cells. Thus, these miRNAs could be developmentally expressed in muscle cells of both organs. A group of five miRNAs showed abundant expression across organs (let-7a, -7b, miR-30b, -30c). let-7 family members have been suggested to play a role in terminal differentiation during development in invertebrates and vertebrates . Overall, about 50% of the expressed miRNAs were assigned to a specific or restricted set of organs. Other miRNAs had a more complex pattern of expression and these were not readily assigned to a particular group.
The majority of miRNAs presented here were previously cloned by Lagos-Quintana and colleagues [2, 3]. Our characterization of organ expression of miRNAs correlates with the organ(s) from which these miRNAS were originally cloned [2, 3]. miRNAs that were abundantly cloned in a particular organ, such as miR-135 and miR-153 from brain , were exclusively detected by northern analysis in mouse and human brain. However, we observed some discrepancies between our expression profiling by northern analysis and expression profiling by cloning [2, 3]. This is particularly so for certain less-abundant miRNAs - those that were represented in size-selected RNA libraries by three or fewer copies. We believe that northern analysis is a more statistically robust method for expression profiling of less abundant miRNAs than cloning frequency is. The panel of organs that we tested in our study did not include all the organs and tissues that miRNAs were cloned from [1–3, 9, 10], and thus some organ-specific or organ-enriched miRNAs may be expressed in additional organs or tissues.
Coordinate expression of closely linked miRNAs
We have also found several cases were closely linked miRNAs are not tightly coexpressed. Closely linked miRNAs such as mir-23b~mir-27b~mir-24/mir-189 exhibited more loosely correlated expression patterns (Figure 3). However, a transcript containing miR-23b~miR-27b~miR-24/miR-189 was detected by northern analysis in humans (gi|4105182), which suggests that these clustered miRNAs probably belong to the same transcriptional unit, but accumulate differentially in vivo as a result of differences in post-transcriptional processing or stability. Other cases of clustered miRNAs with different expression profiles, such as mir-132 and mir-212, could represent closely linked but independent transcription units.
miRNAs can be expressed from long transcripts
We found in GenBank  evidence that single, isolated miRNA genes can be expressed from long transcripts. miR-9/miR-9* and miR-145 were cloned in cDNA libraries from human hypothalamus (gi|28436750) and trachea (gi|21752921), respectively. Both miR-191 and miR-214 were contained in long transcripts cloned in cDNA libraries from mouse neonate head (gi|26096865) and eyeball (gi|26094816), respectively. Where the miRNA transcriptional unit starts is an important consideration since regulatory regions for miRNA transcription may reside kilobases upstream of the predicted hairpin precursor. In other cases, a miRNA may be regulated by a cryptic or nearby promoter. For example, mir-208, which is expressed in the mouse and human heart, resides in an intron of human cardiac α-myosin heavy chain. Perhaps mir-208 is co-transcribed with α-myosin and then processed out.
Some miRNAs are associated with chromosomal rearrangements
Some miRNAs reside in genomic regions that have been associated with a disease state. mir-15 and mir-16 reside on chromosome 13q14, a region associated with chronic lymphocytic leukemia (CLL) (Figure 3b). Calin et al. reported that miR-15 and miR-16 were expressed at reduced levels or unexpressed at all in patients with CLL . Notably, mir-142s/mir-142as are located in the junction of the t(8;17) chromosomal rearrangement. This translocation fuses Bcl3 and Myc genes, thus resulting in an aggressive B-cell leukemia [2, 37]. mir-29 resides on chromosome 7q31. This FRA7G region is frequently altered in different tumor types including prostate, breast, and ovarian cancer . It has also been hypothesized that FRA7G region contains a tumor suppressor gene, which is mutated during the development of these cancers or an oncogene that is amplified . It will be interesting to see if miR-142s/miR-142as and miR-29 expression is altered in patients with a t(8;17) chromosomal rearrangement or mutated FRA7G region, respectively.
A subset of brain-expressed miRNAs is induced in retinoic acid-treated EC cells
Only a subset of the brain-expressed miRNAs were detected in neurons derived from RA-treated EC cells suggesting that other brain-expressed miRNAs are present primarily in nonneuronal cell types such as glia. Alternatively, these other brain-expressed miRNAs may be present in neuronal subtypes not represented in differentiated neurons derived from EC cells. In a recent study, Krichevsky et al. described a series of miRNAs that were developmentally regulated in rat brain during corticogenesis . We observed that the same series of miRNAs was upregulated in EC cells upon RA-treatment. Krichevsky et al. also showed that miR-9/miR-9* were expressed at lower levels in the presenillin-1 knockout mouse, which had an abnormal forebrain development . We observed a strong induction of miR-9/miR-9* expression in P19 cells. Together, these results suggest that miR-9/miR-9* may play an important role during neurogenesis in rodents.
The coordinate activation of miRNAs (miR-9/9*, -103-1, -124a, -124b, -128, -135, -156, -218) during neuronal differentiation represents a global action of RA in the genome, since these miRNAs constitute unlinked loci. This contrasts with the localized repression exerted by RA upon embryonic stem cell-specific miRNAs (miR-290 to miR-295) arranged in two tightly linked loci .
LIN-28 could be a potential target for miRNA regulation in embryonal carcinomas
The mir-125 and let-7 family members were among the brain-expressed miRNAs induced during neuronal differentiation. In C. elegans, lin-4 (the ortholog of mir-125) and let-7 are engaged in a genetic pathway that controls temporal cell fates during larval development [25, 26]. Upregulation of lin-4 RNA during the first larval stage (L1) triggers the progression from early (L1) to late (L2-L3) fate programs . lin-4 RNA binds to the 3' UTR of target mRNAs, including lin-28, and inhibits their translation [27, 45]. LIN-28 is expressed at early stages in neuronal, hypodermal, and intestinal lineages before it is developmentally downregulated by lin-4 RNA. In mammals, LIN-28 protein is downregulated as embryonic development progresses, consistent with a possible conserved role in developmental timing . Interestingly, LIN-28 is present in undifferentiated P19 and NT2/D1 cells, but it is not detected in neuronal or liver tissues . To test if downregulation of LIN-28 could occur in conjunction with neuronal differentiation, western analysis of total protein from lysates of P19 and NT2/D1 cells was performed at various times after RA-treatment. LIN-28 levels began to decrease on about day five after RA-treatment, and almost disappeared by day eight, when cells reached their final state of neuronal differentiation. A similar result was observed in NT2/D1 cells; LIN-28 levels decreased slightly, typically by day 14, and were still detectable at day 21. The kinetics of LIN-28 downregulation in P19 and NT2/D1 cells during RA-induced differentiation parallels the upregulation of miR-125b, let-7a, and miR-218 in these EC cells. The 3' UTRs of the mouse and human Lin-28 mRNAs contain conserved sequences complementary to miR-125b, let-7a, and miR-218 (Figure 5) , consistent with a role for these miRNAs in the downregulation of LIN-28 in NT2/D1 and P19 cells. Yet, we did not detect downregulation, in differentiating P19 cells, of a luciferase reporter gene fused to the mouse Lin-28 3' UTR and driven by a cytomegalovirus (CMV) or thymidine kinase (TK) promoter (data not shown). We also noticed a decrease in endogenous Lin-28 mRNA levels in differentiated neurons derived from P19 cells (data not shown), suggesting that repression of the native Lin-28 gene in this system may be largely transcriptional. These results therefore suggest that, unlike C. elegans larval development, where lin-28 downregulation is primarily caused by translational repression by lin-4 RNA, the decline of Lin-28 during neuronal differentiation of P19 cells involves primarily transcriptional repression. However, the phylogenetic conservation of miRNA sites suggests that translational repression of Lin-28 by let-7a, miR-125b, and/or miR-218 may contribute secondarily to Lin-28 downregulation in the mammalian system.
Myc and Cyclin D1 are well-characterized cases of genes repressed by RA-treatment at the levels of transcription and translation in differentiating neurons [47, 48]. We subjected the conserved blocks between mouse and human Myc and Cyclin D1 3' UTRs to the same computer algorithm applied to Lin-28 3' UTR to identify potential binding sites for RA-induced miRNAs. Potential sites for let-7 and miR-30 family members were found on Myc and Cyclin D1 3' UTRs (data not shown). These findings suggest that translational repression by miRNAs, in combination with transcriptional repression and mRNA turnover, could contribute to the downregulation of growth and cell cycle regulators during neuronal differentiation.
Hundreds of miRNAs have recently been discovered in mammalian genomes. To begin to explore the potential roles of these miRNAs in mammalian development and physiology, we characterized the expression profiles of 119 miRNAs in mouse and human organs (Figure 1). We found that many of these miRNAs exhibit phylogenetically conserved organ-enriched or organ-specific expression. In both mouse and human examined organs, seven miRNAs were expressed specifically in a particular organ and ten miRNAs were greatly enriched in a particular organ over all others (Figure 2). These data associate miRNAs to particular organs and suggest their participation in conserved developmental or physiological pathways within these spatial domains in mouse and human. Interestingly, 13 of these 17 miRNAs were enriched or specifically expressed in brain of both mouse and human. Of these, eight brain miRNAs were also induced during differentiation of neurons derived from human and mouse EC cells treated with RA (Figure 4). These data strongly suggest a conserved role for these miRNAs in the specification and/or progression of neuronal fate in mammals. At least one gene whose protein product decreases during neuronal differentiation, Lin-28, contains potential sites in its 3' UTR for complementary base-pairing by the RA-induced miRNAs let-7a, miR-125b, and miR-218 (Figure 5). However, not only the protein levels but also the RNA levels of Lin-28 decreased upon RA treatment suggesting that other factors must also mediate this process. Unlike C. elegans lin-28 whose translational inhibition by lin-4 RNA is the primary mechanism for its downregulation, miRNAs may provide an auxiliary or reinforcing layer of regulation to completely shut down Lin-28 expression in EC cells. miRNA loss-of-function experiments in these EC cells could prove useful in understanding their regulatory contribution during the process of neuronal differentiation. In the case of targets whose expression is primarily controlled on the translational level, miRNAs could play a primary regulatory role; in other cases, such as mammalian Lin-28, where the primary mode of regulation is transcriptional, translational repression by miRNAs could play an auxiliary, or supplementary regulatory role.
Cell culture and retinoic acid treatment
P19 cells were cultured in αMEM with 7.5% fetal calf serum and 2.5% fetal bovine serum supplemented with streptomycin and penicillin; NT2/D1 cells were cultured in high glucose DME with 10% fetal bovine serum supplemented with penicillin, streptomycin, and glutamine under humidified 5% CO2. P19 cells were treated with 1 μM RA solution as described in ; NT2/D1 were treated with 10 μM RA solution as described in . Control cells were treated with an equal amount of the vehicle dimethyl-sulfoxide.
Frozen dissected organs from six-week-old C57BL/6 mice were a gift from Nathan Watson (Norris Cotton Cancer Center at Dartmouth-Hitchcock Medical Center). Total RNA was extracted from pooled organs of two to four mice or from cell lines with Trizol Reagent following the vendor's recommendations. Total RNA, 5 or 10 μg, was routinely loaded onto 12% urea-polyacrylamide gels as described in . Total RNA from human organs was acquired from the Biochain Institute. Membranes were stripped by boiling in 0.1% SDS and re-hybridized up to five times without observing decreases in quality or intensity of radioactive signals.
StarFire oligonucleotide probes
We used StarFire probes, radioactively labeled with [α-32P]-dATP 6000 Ci/mmol following vendor's recommendations. The complete list of StarFire probes used in this study is enclosed in the additional data file.
Radioactivity contained in northern blot bands was quantified with the ImageQuant software package, and was expressed as a ratio of signal in the band of hybridization to signal in the background adjacent to the band. Hybridization/background ratios of ≤ 1.1 were not reported as positive signals, except when the band was readily detectable by eye (Figures 2, 5). Northern hybridization data were clustered with GeneTraffic 6.2 software package, applying Pearson's hierarchical clustering.
miRNA::target mRNA predictions
Potential RNA::RNA interactions between miRNAs and 3' UTR sequences were assessed by testing 20-nts segments of 3' UTR sequence for their predicted capacity to form RNA secondary structure with a candidate miRNA. Each miRNA sequence was appended to each 20-nucleotide 3' UTR segment by a poly-(N)10 linker, and analyzed for predicted RNA secondary structure using the RNAfold program of the Vienna RNA package . The full length of a 3' UTR sequence was queried by extracting successive 20-nucleotide test segments, tiling along in two-nucleotide steps. RNA folding output generated by ViennaRNA was analyzed using the following software filters to evaluate the strength of the predicted target::miRNA interaction: at least two out of the three first and last bases of the miRNA had to be bound to the target sequence; at least 65% of the miRNA sequence had to be bound to the target sequence; the free energy (ΔG) of the binding had to be greater than -7 Kcal/mole; and the target::miRNA interaction site had to lie within regions of at least 76% identity between mouse and human target 3' UTRs.
The scoring scheme was modeled based on the experimentally confirmed interaction between lin-4 RNA::lin-28 3' UTR in C. elegans. Those predicted miRNA::UTR structures that passed the above filters were further assigned a numerical score based on the following parameters: total number of miRNA bases bound to a target sequence - points for binding were weighed, having a less impact on the score of a mismatch in the central region of the miRNA, which tends to bulge out in documented miRNA/mRNA interactions [23, 45] (denoted (a)); number of consecutive miRNA bases bound to target sequence (denoted (b)); overall ΔG of RNA-RNA interaction (denoted (c)); and additional emphasis for miRNA with the first three and last three bases bound to target sequence (denoted (d)). This is the formula of the overall score = 8a + 2b + 84c + 6d.
The complete list of StarFire probes used in this study is provided in Additional data file 1, available with the complete version of this article, online.
We would like to thank Carol Ringelberg for assistance with data clustering analysis and Elena Bryleva for helpful comments on this manuscript. This work was supported by Public Health Service grant GM34028 to V.A., National Institutes of Health and National Cancer Institute grants RO1-CA87546 and RO1-CA62275 to E.D., The Lance Armstrong Foundation to S.J.F., and National Institutes of Health grant T32-CA09658 to I.P.
- Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T: Identification of novel genes coding for small expressed RNAs.Science 2001, 294:853–858.View ArticleGoogle Scholar
- Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T: Identification of tissue-specific microRNAs from mouse.Curr Biol 2002, 12:735–739.View ArticleGoogle Scholar
- Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T: New microRNAs from mouse and human.RNA 2003, 9:175–179.View ArticleGoogle Scholar
- Lai EC, Tomancak P, Williams RW, Rubin GM: Computational identification ofDrosophilamicroRNA genes.Genome Biol 2003, 4:R42.View ArticleGoogle Scholar
- Lau NC, Lim LP, Weinstein EG, Bartel DP: An abundant class of tiny RNAs with probable regulatory roles inCaenorhabditis elegans.Science 2001, 294:858–862.View ArticleGoogle Scholar
- Lee RC, Ambros V: An extensive class of small RNAs inCaenorhabditis elegans.Science 2001, 294:862–864.View ArticleGoogle Scholar
- Lim LP, Lau NC, Weinstein EG, Abdelhakim A, Yekta S, Rhoades MW, Burge CB, Bartel DP: The microRNAs ofCaenorhabditis elegans.Genes Dev 2003, 17:991–1008.View ArticleGoogle Scholar
- Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP: Vertebrate microRNA genes.Science 2003, 299:1540.View ArticleGoogle Scholar
- Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G: miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.Genes Dev 2002, 16:720–728.View ArticleGoogle Scholar
- Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfuss G: Numerous microRNPs in neuronal cells containing novel microRNAs.RNA 2003, 9:180–186.View ArticleGoogle Scholar
- Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP: MicroRNAs in plants.Genes Dev 2002, 16:1616–1626.View ArticleGoogle Scholar
- Llave C, Kasschau KD, Rector MA, Carrington JC: Endogenous and silencing-associated small RNAs in plants.Plant Cell 2002, 14:1605–1619.View ArticleGoogle Scholar
- Ke XS, Liu CM, Liu DP, Liang CC: MicroRNAs: key participants in gene regulatory networks.Curr Opin Chem Biol 2003, 7:516–523.View ArticleGoogle Scholar
- Sempere LF, Sokol NS, Dubrovsky EB, Berger EM, Ambros V: Temporal regulation of microRNA expression inDrosophila melanogastermediated by hormonal signals and Broad-Complex gene activity.Dev Biol 2003, 259:9–18.View ArticleGoogle Scholar
- Lee Y, Jeon K, Lee JT, Kim S, Kim VN: MicroRNA maturation: stepwise processing and subcellular localization.EMBO J 2002, 21:4663–4670.View ArticleGoogle Scholar
- Pasquinelli AE, Reinhart BJ, Slack F, Martindale MQ, Kuroda MI, Maller B, Hayward DC, Ball EE, Degnan B, Muller P, et al.: Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA.Nature 2000, 408:86–89.View ArticleGoogle Scholar
- Krichevsky AM, King KS, Donahue CP, Khrapko K, Kosik KS: A microRNA array reveals extensive regulation of microRNAs during brain development.RNA 2003, 9:1274–1281.View ArticleGoogle Scholar
- Carrington JC, Ambros V: Role of microRNAs in plant and animal development.Science 2003, 301:336–338.View ArticleGoogle Scholar
- Chen X: A microRNA as a translational repressor of APETALA2 inArabidopsisflower development.Science 2003, 101126/science.1088060.Google Scholar
- Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D: Control of leaf morphogenesis by microRNAs.Nature 2003, 425:257–263.View ArticleGoogle Scholar
- Tang G, Reinhart BJ, Bartel DP, Zamore PD: A biochemical framework for RNA silencing in plants.Genes Dev 2003, 17:49–63.View ArticleGoogle Scholar
- Xie Z, Kasschau KD, Carrington JC: Negative feedback regulation of Dicer-Like1 inArabidopsisby microRNA-guided mRNA degradation.Curr Biol 2003, 13:784–789.View ArticleGoogle Scholar
- Lee RC, Feinbaum RL, Ambros V: TheC. elegansheterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.Cell 1993, 75:843–854.View ArticleGoogle Scholar
- Feinbaum R, Ambros V: The timing of lin-4 RNA accumulation controls the timing of postembryonic developmental events inCaenorhabditis elegans.Dev Biol 1999, 210:87–95.View ArticleGoogle Scholar
- Pasquinelli AE, Ruvkun G: Control of developmental timing by microRNAs and their targets.Annu Rev Cell Dev Biol 2002, 18:495–513.View ArticleGoogle Scholar
- Rougvie AE: Control of developmental timing in animals.Nat Rev Genet 2001, 2:690–701.View ArticleGoogle Scholar
- Olsen PH, Ambros V: The lin-4 regulatory RNA controls developmental timing inCaenorhabditis elegansby blocking LIN-14 protein synthesis after the initiation of translation.Dev Biol 1999, 216:671–680.View ArticleGoogle Scholar
- Seggerson K, Tang L, Moss EG: Two genetic circuits repress theCaenorhabditis elegansheterochronic gene lin-28 after translation initiation.Dev Biol 2002, 243:215–225.View ArticleGoogle Scholar
- Slack FJ, Basson M, Liu Z, Ambros V, Horvitz HR, Ruvkun G: The lin-41 RBCC gene acts in theC. elegansheterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor.Mol Cell 2000, 5:659–669.View ArticleGoogle Scholar
- Abrahante JE, Daul AL, Li M, Volk ML, Tennessen JM, Miller EA, Rougvie AE: TheCaenorhabditis eleganshunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs.Dev Cell 2003, 4:625–637.View ArticleGoogle Scholar
- Lin SY, Johnson SM, Abraham M, Vella MC, Pasquinelli A, Gamberi C, Gottlieb E, Slack FJ: TheC eleganshunchback homolog, hbl-1, controls temporal patterning and is a probable microRNA target.Dev Cell 2003, 4:639–650.View ArticleGoogle Scholar
- Brennecke J, Hipfner DR, Stark A, Russell RB, Cohen SM: bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid inDrosophila.Cell 2003, 113:25–36.View ArticleGoogle Scholar
- Xu P, Vernooy SY, Guo M, Hay BA: TheDrosophilamicroRNA mir-14 suppresses cell death and is required for normal fat metabolism.Curr Biol 2003, 13:790–795.View ArticleGoogle Scholar
- Houbaviy HB, Murray MF, Sharp PA: Embryonic stem cell-specific MicroRNAs.Dev Cell 2003, 5:351–358.View ArticleGoogle Scholar
- National Center for Biotechnology Information[http://www.ncbi.nlm.nih.gov/]
- Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, Aldler H, Rattan S, Keating M, Rai K, et al.: Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.Proc Natl Acad Sci USA 2002, 99:15524–15529.View ArticleGoogle Scholar
- Gauwerky CE, Huebner K, Isobe M, Nowell PC, Croce CM: Activation of MYC in a masked t(8;17) translocation results in an aggressive B-cell leukemia.Proc Natl Acad Sci USA 1989, 86:8867–8871.View ArticleGoogle Scholar
- Huang H, Qian J, Proffit J, Wilber K, Jenkins R, Smith DI: FRA7G extends over a broad region: coincidence of human endogenous retroviral sequences (HERV-H) and small polydispersed circular DNAs (spcDNA) and fragile sites.Oncogene 1998, 16:2311–2319.View ArticleGoogle Scholar
- Jones-Villeneuve EM, Rudnicki MA, Harris JF, McBurney MW: Retinoic acid-induced neural differentiation of embryonal carcinoma cells.Mol Cell Biol 1983, 3:2271–2279.View ArticleGoogle Scholar
- Jones-Villeneuve EM, McBurney MW, Rogers KA, Kalnins VI: Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells.J Cell Biol 1982, 94:253–262.View ArticleGoogle Scholar
- Andrews PW: Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell linein vitro.Dev Biol 1984, 103:285–293.View ArticleGoogle Scholar
- Bain G, Ray WJ, Yao M, Gottlieb DI: From embryonal carcinoma cells to neurons: the P19 pathway.Bioessays 1994, 16:343–348.View ArticleGoogle Scholar
- Andrews PW: Teratocarcinomas and human embryology: pluripotent human EC cell lines. Review article.APMIS 1998, 106:158–167.View ArticleGoogle Scholar
- Wei Y, Harris T, Childs G: Global gene expression patterns during neural differentiation of P19 embryonic carcinoma cells.Differentiation 2002, 70:204–219.View ArticleGoogle Scholar
- Moss EG, Lee RC, Ambros V: The cold shock domain protein LIN-28 controls developmental timing inC. elegansand is regulated by the lin-4 RNA.Cell 1997, 88:637–646.View ArticleGoogle Scholar
- Moss EG, Tang L: Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites.Dev Biol 2003, 258:432–442.View ArticleGoogle Scholar
- Miller WH Jr, Moy D, Li A, Grippo JF, Dmitrovsky E: Retinoic acid induces down-regulation of several growth factors and proto-oncogenes in a human embryonal cancer cell line.Oncogene 1990, 5:511–517.Google Scholar
- Spinella MJ, Freemantle SJ, Sekula D, Chang JH, Christie AJ, Dmitrovsky E: Retinoic acid promotes ubiquitination and proteolysis of cyclin D1 during induced tumor cell differentiation.J Biol Chem 1999, 274:22013–22018.View ArticleGoogle Scholar
- Freemantle SJ, Kerley JS, Olsen SL, Gross RH, Spinella MJ: Developmentally-related candidate retinoic acid target genes regulated early during neuronal differentiation of human embryonal carcinoma.Oncogene 2002, 21:2880–2889.View ArticleGoogle Scholar
- Sempere LF, Dubrovsky EB, Dubrovskaya VA, Berger EM, Ambros V: The expression of the let-7 small regulatory RNA is controlled by ecdysone during metamorphosis inDrosophila melanogaster.Dev Biol 2002, 244:170–179.View ArticleGoogle Scholar
- Vienna RNA package[http://www.tbi.univie.ac.at/~ivo/RNA/]
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