Taste and pheromone perception in mammals and flies
© BioMed Central Ltd 2003
Published: 30 June 2003
The olfactory systems of insects and mammals have analogous anatomical features and use similar molecular logic for olfactory coding. The molecular underpinnings of the chemosensory systems that detect taste and pheromone cues have only recently been characterized. Comparison of these systems in Drosophila and mouse uncovers clear differences and a few surprising similarities.
Feeding is initiated in terrestrial animals with the detection of volatile (airborne) chemicals. Generally referred to as odorants, these chemicals are released from food sources towards which animals move by monitoring the concentration gradient of a particular odor. Naturally, noxious volatile substances are also detected by the same sensory epithelia, in this case leading to avoidance of the odor source. In both mammals and insects, volatile cues are detected by odorant receptor proteins, which are G-protein-coupled receptors (GPCRs) encoded by a single large gene family expressed in the cognate olfactory epithelia in the nose and antenna of mammals and insects, respectively. The odorant receptor gene family consists of about 1,000 genes in mice and 60 in Drosophila. Surprisingly, the odorant receptor genes in mammals and insects are not related to each other at the sequence level (beyond encoding GPCRs), even though they recognize a largely overlapping array of chemical compounds. The olfactory systems of insects and mammals have analogous anatomical features and use similar molecular logic for olfactory coding, however .
Once a potential food source is identified, animals taste it by means of contact between chemicals from the source and chemosensory receptors located in the gustatory (taste) organs, predominantly the tongue in mice and the labellum (proboscis) in Drosophila (both species also have secondary taste organs). Distinct sets of taste-receptor proteins in specific taste cells allow the organism to discriminate between 'delicious' food, generally associated with rich nutrition, and bitter-tasting substrates, typically present in contaminated, non-edible food sources.
Courtship and mating are also initiated by chemosensory signals, better known as pheromones, although other sensory systems are also important for these behaviors. Pheromones are sometimes sex-specific and are secreted by specialized glands or cells to trigger distinct behavioral and endocrine responses in members of the same and opposite sexes of the same species. Specialized sensory structures or cells recognize pheromones: the mammalian vomeronasal organ is deeply embedded in the nasal cavity , while a subset of gustatory and possibly olfactory neurons are thought to be responsible for the detection of pheromones in Drosophila [3–5]. In both mice and Drosophila, the major pheromones are non-volatile chemicals, so detection of pheromones requires direct contact of the sensory cells with the pheromone source [6–8]. Candidate pheromone-receptor genes have been identified in mammals, but only a few have recently been functionally characterized .
The main focus of this article is the recent progress in our understanding of the taste and pheromone receptors in mouse, humans and Drosophila. Despite the rather different anatomical structures and organs involved in the detection of tastes and pheromones, and the different structures of taste and pheromone receptors, a few intriguing parallels and similarities have emerged from recent studies. We note that the mode and role of pheromone recognition has diverged significantly within mammal and insect species, so the findings summarized here might not be general to all genera within each group of animals.
The mouse chemosensory system
Multiple classes of taste receptors
Taste and pheromone receptor gene (sub-)families in mice and Drosophila
Number of intact genes
Conservation* within each family
Sites of expression
Known ligands (examples)
3 (human) 3 (mouse)
Taste cells in the tongue; T1R1 plus T1R3 or T1R2 plus T1R3 are preferentially coexpressed, with few cells expressing all three genes
Sweet chemicals (T1R2 plus T1R3) L-amino acids, umami chemicals (T1R1 plus T1R3)
Similar to V2R (20-30% identical)
28 (human); approximately 40 (mouse)
25% to more than 80%
Taste cells in the tongue; most cells express many, if not all, T2R genes
Bitter compounds, such as cycloheximide (mouse T2R5), salicin (human T2R16), and PTC (human T2R38)
Similar to V1R (15-20% identical)
5 (human) 137 (mouse)
25% to nearly 100%
Basal layer in the vomeronasal organ; probably one V1R gene expressed per cell
2-heptanone (V1Rb1), 6-hydroxy-6-methyl-3-heptanone, n-pentylacetate, and isobutylamine
Similar to T2R (15-20% identical); important for maternal aggression
None in human; approximately 140 in mouse
25% to nearly 100%
Apical layer in the vomeronasal organ; probably one V2R gene expressed per cell
Similar to T1R (20-30% identical); forms a complex with MHC class Ib and β2-microglobulin; may be required for aggression between males
None in human; 9 expressed in the mouse
40% to more than 80%
Apical layer in the vomeronasal organ; one or a few MHC 1b genes expressed per cell; coexpressed with a V2R gene
None (in the VNO)
Forms a complex with β2-microglobulin and V2Rs; may be required for aggression between males
Spatially defined in one or several taste organs (labellum, labral and cibarial sense organs, legs, wings, and female genitalia)
Sugars and presumably other classes of compounds, including pheromones
Distantly related to olfactory receptor genes (Or83b); gustatory receptor probably recognize the whole spectrum of small soluble ligands; up to 20 subfamilies (with 1-8 genes each)
Foreleg and labellum (Gr5a), possibly other taste sensory organs
The knockout fly has lower sensitivity to trehalose; member of a subfamily of 7 genes
Three members of the T1R family have been isolated from rat, mouse and human . T1Rs are 30-40% identical to each other at the amino-acid level, and the genes encoding them are located in the same region on chromosome 4 in mouse (chromosome 1 in human). Like GABAb receptors, T1Rs also form heterodimers (or even more complex multimers). Expression studies have revealed that taste cells either coexpress T1R1 and T1R3 or T1R2 and T1R3, but not T1R1 and T1R2 [22, 23], and it has recently been shown that these distinct combinations of T1Rs bind to different sets of compounds, corresponding to sweet and umami substances [23–25]: in mouse, T1R2-T1R3 hetero(di)mers respond to sugars and sweeteners, whereas T1R1-T1R3 hetero(di)mers are activated by many L-amino acids, including L-glutamate and L-arginine, both of which are umami-tasting to humans. It is not known, however, whether these other L-amino acids are perceived as umami taste by mice. In humans, T1R1 and T1Rs receptors appear to be less broadly tuned and are sensitive mainly to L-glutamate and L-arginine . It has been suggested that T1R1 and T1R2 may determine the ligand specificity of each heterodimer. T1R3 may also have distinct roles in sweet and umami sensation, however, because some polymorphisms in the mouse T1R3 gene affect only sweet sensation but not L-amino-acid perception .
The T2Rs, responsible for the detection of bitter-tasting compounds, have no detectable sequence similarity to T1Rs and form a larger family of GPCRs than the T1Rs . T2R proteins are from 25% to more than 80% identical to each other, and their genes are located in gene clusters on chromosomes 6 in mouse (chromosomes 7 and 12 in humans). In contrast to T1Rs, T2Rs lack a long amino-terminal extracellular domain. There are 28 potentially functional T2R genes in human and at least 33 in mouse [26–28]. Mouse T2R5 is a receptor for cycloheximide  and human T2R16 responds to several bitter compounds, all of which have a similar chemical signature: a hydrophobic residue attached to glucose via a β-glycosidic bond  (see Table 1). It has long been known that some people taste the bitter compound phenylthiocarbamide (PTC) at much lower concentrations than others. This recessive trait has been mapped to a region on chromosome 7 and is referred to as the PTC locus.
Interestingly, a T2R-encoding gene, T2R38, is closely linked to the PTC locus. Recently, Kim et al.  have shown that PTC sensitivity correlates with polymorphisms in the T2R38 gene that are located to positions 49 (intracellular domain 2) and 262 (transmembrane domain 6). Expression of T2Rs has been extensively analyzed, and it has been shown that individual taste cells express a large number of, if not all, different T2R genes . This might explain the poorly developed ability of mice and humans to discriminate between different bitter-tasting compounds.
Unlike sweet and bitter tastes, sour and salt tastes are thought to be mediated by ion channels. The mDEG-1 and HCN1/4 ion channels have been proposed to act as sour receptors, whereas salty taste appears to be mediated by the amiloride-sensitive sodium channels [12, 33, 34]. There are as yet no functional data in vivo, however, that link these channels directly to the sour and salty tastes.
The vomeronasal organ is the major pheromone-detecting organ in rodents and probably most other mammals (except humans), although some pheromones are detected by the olfactory epithelium. The sensory epithelium of the vomeronasal organ is arranged in a basal and apical layer, each of which expresses one type of pheromone receptor belonging to either the V1R or the V2R family of GPCRs (Figure 1a and Table 1) [11, 35]. Like olfactory neurons, but unlike taste cells, each neuron in the vomeronasal organ expresses only one (or, at most, a few ) receptor. The apically expressed V1R family has 135 members in the mouse, which have from 25% to nearly 100% amino-acid sequence identity to each other, and their genes are organized in several gene clusters . These receptors are related to the T2R receptor family (with 15-20% amino-acid sequence identity), suggesting that the genes encoding these two receptor families derived from a common ancestral gene. Boschat et al.  identified a putative pheromone, 2-heptanone, as a ligand for one member of the V1R family (V1Rb2). They used knockin mice in which all V1Rb2-expressing cells also express the green fluorescent protein (GFP) as a cell marker. Using calcium-imaging experiments to monitor neuronal responses in terms of the release of Ca2+ from intracellular stores, they showed that GFP-positive neurons in the vomeronasal organ respond specifically to 2-heptanone. Moreover, they showed that GFP-expressing cells of mice in which the V1Rb2 gene was replaced by the GFP gene no longer responded to 2-heptanone. In another study that addressed the function of V1R genes, Del Punta et al.  deleted about 600 kilobases (kb) of a gene cluster containing 16 different V1R genes and created transgenic mice with this deletion. The most obvious phenotype in these mice is a reduction of maternal aggressive behavior. Furthermore, the vomeronasal organs of these knockout mice do not respond to three out of eight putative pheromones tested. Curiously, even though the deleted gene cluster includes V1Rb2, the vomeronasal organs of the knockout mice nevertheless respond to 2-heptanone, suggesting that multiple receptors might recognize and respond to this compound.
A handful of putative V1R genes have been reported in the human genome, but humans appear to lack a functional vomeronasal organ, as well as a functional TRPC2 ion channel , which is required for the function of the vomeronasal organ in the mouse [40, 41]. It is therefore not clear whether the few human V1R sequences that have been identified are expressed as functional receptors, in tissues, such as the main olfactory epithelium.
Members of the second pheromone receptor gene family, the V2Rs, are expressed in the basal layer of the vomeronasal organ. Like the V1Rs, V2R proteins are between 25% and nearly 100% identical to each other and the genes encoding them are also organized in several gene clusters. Interestingly, the V2Rs are also related to taste receptors, in this case to the T1Rs, with which they have 20-30% amino-acid sequence identity. In the mouse the number of genes encoding members of the V2R family is estimated to be between 100 and 200, and no functional V2R genes have yet been reported in humans.
Recently, two groups [42, 43] have reported an unusual class of major histocompatibility complex (MHC) class I genes that are expressed in a subset of neurons in the vomeronasal organ. MHC class I proteins form a complex with β2-microglobulin and are better known for their critical role in antigen presentation in the immune system. These new studies [42, 43] showed that nine MHC class I genes of the M10 and M1 subclass are exclusively expressed by the vomeronasal organ. Moreover, each of these MHC genes is differentially expressed in a subset of neurons in the vomeronasal organ that also express one of the V2R genes. Importantly, a given V2R gene seems to be coexpressed with a particular MHC gene, and, in the vomeronasal organ, the MHC proteins and β2-microglobulin appear to form a complex with V2R proteins as shown by immunoprecipitation studies . Furthermore, protein localization experiments have shown that the MHC-β2-microglobulin complex is essential for V2R protein expression on the dendritic surface of neurons in the vomeronasal organ. The importance of the MHC-β2-microglobulin complex for cell-surface expression of V2R proteins was further confirmed in tissue-culture experiments. When V2R genes are expressed in a testis cell line, V2R receptor proteins remain inside the cell, but when the cells are cotransfected with MHC and β2-microglobulin genes, V2R receptor proteins can be detected on the cell surface. Even though β2-microglobulin is essential for normal aggressive behavior between males, as shown in β2-microglobulin knockout mice, no specific function has yet been assigned to any of the V2R or MHC class I receptors expressed in the vomeronasal organ.
The Drosophilachemosensory system
The chemosensory system of the Drosophila adult can be functionally separated into the olfactory as well as the gustatory and pheromone systems (Figure 1b) . Volatile olfactory cues are detected by about 60 olfactory receptor proteins expressed in two pairs of appendages located on the fly's head - the third antennal segment and the maxillary palps [44–46]. Conversely, the gustatory system is widely dispersed throughout the fly's body . In the adult, the two labial palps (proboscis), considered to be the 'insect tongue', are the major taste organs. Each palp harbors three rows of about 10 to 13 taste sensory bristles, containing four (type A) or two (type B) gustatory neurons and a single mechanosensory neuron. Electrophysiological studies on type A bristles have revealed that these neurons respond to distinct stimuli (sugar, water, and high and low salt concentrations) [47–49]. In addition, there are about 30 neurons arranged individually at the base of taste pegs between pseudotracheae on the surface of each labial palp. Finally, there are three sensilla clusters in the pharynx (the labral sense organ and the distal and proximal cibarial sense organs) that are composed of a total of 40 additional neurons. An adult fly has therefore about 260 outer and 40 internal taste sensory neurons in the labellum . Taste bristles contain a pore that provides access for substrates to the dendrite of the neurons. In principle, the Drosophila taste bristles are analogous to the mammalian taste buds: both mammals and Drosophila have distinct functional taste units that are mainly classified on the basis of structure and location. Interestingly, mammals also have clusters of taste cells in the pharynx, which might correspond functionally to the small group of taste neurons in the fly pharynx and be involved in the sucking reflex.
In addition to the neurons in the labellum, at least twice as many gustatory receptor neurons can be found in taste sensilla on the tarsi of all legs and the anterior wing margins. Interestingly, males have a significantly larger number of gustatory bristles on their forelegs than females (50 versus 37), and a few taste bristles have also been described in the female genitalia [5, 50, 51]. The large number of gustatory receptor neurons in the legs and wings indicates that they contribute significantly to the fly's sensory intake.
The gustatory receptor gene family
The gustatory receptors in Drosophila are encoded by a family of about 70 genes [52–54]; most of the predicted gustatory receptor proteins are 15-30% similar to each other, but the most highly conserved receptors have up to 75% sequence similarity. The gustatory receptors can be grouped into several subfamilies on the basis of sequence similarity, with members within each subfamily having 33% or more identity. Genes within a subfamily are often clustered in the genome; in fact, about half of the 70 genes are found in arrays containing up to six genes each. Surprisingly, genes within a cluster appear not to be expressed in an orchestrated fashion: they are regulated independently and can be expressed in distinct taste organs . Gustatory receptor genes arrayed head-to-tail (in the same 5' to 3' orientation) in two loci appear to be alternatively spliced: alternative exons at the 5' end of the genes, encoding the amino terminus, are spliced to common 3' exon(s) that encode the carboxyl termini, including the seventh transmembrane domain and the short cytoplasmic tail of the receptor; these 3' exons are present in all transcripts [52–54].
Expression of some gustatory receptor genes has been analyzed using a transgenic approach that takes advantage of the UAS-Gal4 system [52, 53]. The putative promoter of a gustatory receptor gene (the 5' region upstream of the corresponding coding sequence) is fused to the coding sequence of the yeast transcriptional activator gal4, which tightly controls the expression of an appropriate reporter gene (the gfp or lacZ gene linked to the Gal4 target sequence UAS . Even though expression of only about ten gustatory receptor genes has been reported, some general rules have emerged from these investigations. First, and foremost, for all but one gustatory receptors, expression is restricted to a very small fraction (1-2%) of the gustatory receptor neurons, and the neurons that express each gene are mostly located in one or two taste organs; the exception to this rule is Gr22e, which is expressed in about 5 to 10% of gustatory receptor neurons across all taste organs . Second, most receptors appear to be expressed in a single neuron within a taste bristle. Third, gustatory receptor genes are not coexpressed in large numbers in the same cells. Finally, as mentioned earlier, even closely related and clustered gustatory receptor genes appear not to be expressed in a coordinated fashion - they are expressed in different gustatory receptor neurons within a given taste organ, such as the labellum, or in entirely different taste organs. For example, members of the Gr22 gene family, which consists of six tightly clustered genes (Gr22a-Gr22f), are only expressed in the labellum (Gr22a and Gr22f), in the labellum and legs (Gr22c), in the labellum, legs and cibarial sense organ (Gr22b) or in the entire taste system, including the wings (Gr22e). This is in contrast to the situation for mammalian taste receptors, especially the T2Rs, many of which are expressed in single taste cells. The establishment of discrete sets of sensory neurons in the Drosophila taste system that express one or a small number of distinct receptors provides a basis for the discrimination of potentially many different substrates.
Detection of sugars
Direct evidence that the gustatory receptor gene family encode taste receptors was obtained from genetic studies, in which a gene conferring sensitivity to the sugar trehalose was mapped to a single locus on the X chromosome that was later identified as the Gr5a gene [56–58]. Trehalose is an abundant carbohydrate generated as a metabolic by-product in yeast, which is a major food source of Drosophila melanogaster. Sequence analysis of the Gr5a genes of numerous strains with reduced sensitivity to trehalose (tre- strains) identified single point mutations in the Gr5a coding sequence [57, 58]. Moreover, deletions of the Gr5a gene were recently recovered and shown to produce the same reduction in sensitivity as observed in the initially isolated tre- strains, suggesting that their mutations were in fact null mutations. Final proof that Gr5a is indeed a functional trehalose receptor was provided with a Gr5a transgene derived from a highly sensitive strain, which rescued the phenotype of a tre- strain . The cellular expression of Gr5a has not been assessed yet, however, and it remains to be seen whether this receptor is expressed in a pattern analogous to other gustatory receptor genes.
Even though no other specific ligands for gustatory receptors have been identified, it is safe to say that they belong to distinct chemical classes, because the perhaps a dozen biologically relevant sugars are not numerous enough to require a repertoire of 70 proteins for detection. Gr5a is one of eight members of a subfamily, consisting of Gr5a, Gr61a and six additional genes clustered in region 64 of the left arm of the third chromosome (Gr64a - Gr64f). The proteins encoded by these eight genes share up to 75% sequence similarity to each other and it is likely that they represent a group of receptors involved in the detection of structurally related compounds, such as various sugars. Future expression and functional studies will be necessary to define the taste sensilla and gustatory receptor neurons in which all these receptors are expressed and to identify the specific sugar ligands they recognize. It will be interesting to see whether individual receptors in this subfamily are coexpressed; if so, distinct sweet receptors in Drosophila might function as dimers or multimers, like the mammalian T1Rs. In any case, it is likely that other subfamilies recognize a variety of different non-sweet substrates, including pheromones.
Detection of bitter substrates, salts, acids and pheromones
Electrophysiological as well as behavioral studies have revealed that alkaloids and other compounds that taste bitter to humans, salts and acids are also recognized by neurons of the taste bristles located in the various taste organs of the fly [47–49]. The observation that different gustatory receptor genes are expressed in apparently distinct subsets of taste neurons in Drosophila suggests that some of the gustatory receptor proteins recognize non-sugar compounds; this is further supported by the fact that the number of gustatory receptors (70) by far exceeds the number of biologically relevant sugars encountered in the wild. Thus, one likely scenario is that distinct gustatory receptor subfamilies recognize distinct groups of chemical compounds present in the food or the environment in general.
An intriguing, albeit still hypothetical, function for some gustatory receptor genes (or a gustatory receptor subfamily) might include a role in the detection of pheromones. This hypothesis is mainly supported by the fly's anatomy as pheromone perception in Drosophila is not mediated by a specialized organ (such as the vomeronasal organ of mice) but appears to be directly mediated by a subpopulation of taste neurons located in the labial palps as well as the forelegs, both of which are extensively employed by the male during the elaborate male courtship ritual [3, 4]. Moreover, specialized taste bristles in the male forelegs are further implicated by the observation that they are more abundant in males (approximately 50) than females (about 37) [5, 50, 51]. It is therefore likely that neurons of these bristles express pheromone receptors that may be sex-specific and that are crucially involved in the detection of female pheromones. But systematic expression analyses of all gustatory receptor genes will be necessary to identify among them potential candidates for pheromone receptors, and future studies involving live markers of neuronal activation, such as G-CaMP (a calcium-sensitive calmodulin-GFP fusion protein) [59–61], might aid in the identification the ligands for these receptors; it is possible that these ligands are among the known Drosophila pheromones, which are long-chain hydrocarbons [6, 7, 62, 63].
In conclusion, although many genetic pathways and genes are conserved between insects and mammals, including the sensory receptor proteins involved in the detection of light (rhodopsins) and of touch and pain (specific ion channels), the chemosensory receptors of the olfactory, taste and pheromone systems of insects and mammals have no sequence similarity. This is rather surprising, given that many mammals and insects exhibit similar and overlapping profiles of odor and taste preference. But despite the lack of sequence similarity between the cognate receptor proteins, striking similarities in the logic of olfactory sensory coding have been established [64, 65].
There are significant differences in taste and pheromone perception between mammals and Drosophila, however. For example, the largely overlapping receptor expression profile of mammalian taste receptors is in striking contrast to the defined expression map of the gustatory receptor genes in the fly. Even though little is known about the organization of the taste-processing centers in the brains of mammals and flies, the distinct expression profile of gustatory receptor genes in the taste sensory system of Drosophila might be translated into a complex map in the subesophageal ganglion in the fly's brain and hence afford at least the possibility of rather sophisticated taste discrimination, a feature not associated with the mammalian taste system.
Should the Drosophila pheromone receptors emerge as a subfamily of the gustatory receptors, then at least one, and rather striking, similarity between the contact chemosensory receptors of mice and Drosophila would become apparent, namely the evolutionary conservation of taste and pheromone receptors in both species.
One common goal in the near future for both the mammalian and Drosophila taste- and pheromone-receptor fields is the 'de-orphanization' of these receptors, as we still lack broad knowledge of their cognate ligands. A second important task will be to elucidate how taste information is encoded in the brain. Of particular interest will be to investigate the taste discrimination of Drosophila, which, on the basis of expression data of gustatory receptor genes, might quite possibly accommodate more than just the five taste modalities known to humans. Finally, how taste receptors signal their activation is only partly understood in mammals and completely unknown in Drosophila; identifying the signaling components associated with these receptors will therefore be another field of intense study on the way to demystifying taste and pheromone perception.
- Hildebrand J, Shepherd G: Mechanisms of olfactory discrimination: converging evidence for common principles across phyla. Ann Rev Neurosci. 1997, 20: 595-631. 10.1146/annurev.neuro.20.1.595.PubMedView ArticleGoogle Scholar
- Keverne EB: The vomeronasal organ. Science. 1999, 286: 716-720. 10.1126/science.286.5440.716.PubMedView ArticleGoogle Scholar
- Greenspan RJ, Ferveur JF: Courtship in Drosophila. Ann Rev Genet. 2000, 34: 205-232. 10.1146/annurev.genet.34.1.205.PubMedView ArticleGoogle Scholar
- Hall JC: The mating of a fly. Science. 1994, 264: 1702-1714.PubMedView ArticleGoogle Scholar
- Stocker RF: The organization of the chemosensory system in Drosophila melanogaster : a review. Cell Tissue Res. 1994, 275: 3-26.PubMedView ArticleGoogle Scholar
- Ferveur JF, Sureau G: Simultaneous influence on male courtship of stimulatory and inhibitory pheromones produced by live sex-mosaic Drosophila melanogaster. Proc R Soc Lond B Biol Sci. 1996, 263: 967-973.View ArticleGoogle Scholar
- Ferveur JF, Jallon JM: Genetic control of male cuticular hydrocarbons in Drosophila melanogaster. Genet Res. 1996, 67: 211-218.PubMedView ArticleGoogle Scholar
- Luo M, Fee MS, Katz LC: Encoding pheromonal signals in the accessory olfactory bulb of behaving mice. Science. 2003, 299: 1196-1201. 10.1126/science.1082133.PubMedView ArticleGoogle Scholar
- Del Punta K, Leinders-Zufall T, Rodriguez I, Jukam D, Wysocki CJ, Ogawa S, Zufall F, Mombaerts P: Deficient pheromone responses in mice lacking a cluster of vomeronasal receptor genes. Nature. 2002, 419: 70-74. 10.1038/nature00955.PubMedView ArticleGoogle Scholar
- Axel RL: The molecular logic of smell. Sci Am. 1995, 273: 154-159.PubMedView ArticleGoogle Scholar
- Buck LB: The molecular architecture of odor and pheromone sensing in mammals. Cell. 2000, 100: 611-618.PubMedView ArticleGoogle Scholar
- Lindemann B: Taste reception. Physiol Rev. 1996, 76: 718-766.PubMedGoogle Scholar
- Halpern M: The organization and function of the vomeronasal system. Ann Rev Neurosci. 1987, 10: 325-362. 10.1146/annurev.neuro.10.1.325.PubMedView ArticleGoogle Scholar
- Sam M, Vora S, Malnic B, Ma W, Novotny MV, Buck LB: Neuropharmacology. Odorants may arouse instinctive behaviours. Nature. 2001, 412: 142-10.1038/35084137.PubMedView ArticleGoogle Scholar
- Trinh K, Storm DR: Vomeronasal organ detects odorants in absence of signaling through main olfactory epithelium. Nat Neurosci. 2003, 6: 519-525.PubMedGoogle Scholar
- Margolskee RF: Molecular mechanisms of bitter and sweet taste transduction. J Biol Chem. 2002, 277: 1-4. 10.1074/jbc.R100054200.PubMedView ArticleGoogle Scholar
- Zhang Y, Hoon MA, Chandrashekar J, Mueller KL, Cook B, Wu D, Zuker CS, Ryba NJ: Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell. 2003, 112: 293-301.PubMedView ArticleGoogle Scholar
- Montmayeur JP, Matsunami H: Receptors for bitter and sweet taste. Curr Opin Neurobiol. 2002, 12: 366-371. 10.1016/S0959-4388(02)00345-8.PubMedView ArticleGoogle Scholar
- Kunishima N, Shimada Y, Tsuji Y, Sato T, Yamamoto M, Kumasaka T, Nakanishi S, Jingami H, Morikawa K: Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor. Nature. 2000, 407: 971-977. 10.1038/35039564.PubMedView ArticleGoogle Scholar
- Margeta-Mitrovic M, Jan YN, Jan LY: A trafficking checkpoint controls GABA(B) receptor heterodimerization. Neuron. 2000, 27: 97-106.PubMedView ArticleGoogle Scholar
- Margeta-Mitrovic M, Jan YN, Jan LY: Function of GB1 and GB2 subunits in G protein coupling of GABA(B) receptors. Proc Natl Acad Sci USA. 2001, 98: 14649-14654. 10.1073/pnas.251554498.PubMedPubMed CentralView ArticleGoogle Scholar
- Montmayeur JP, Liberles SD, Matsunami H, Buck LB: A candidate taste receptor gene near a sweet taste locus. Nat Neurosci. 2001, 4: 492-498.PubMedGoogle Scholar
- Nelson G, Hoon MA, Chandrashekar J, Zhang Y, Ryba NJ, Zuker CS: Mammalian sweet taste receptors. Cell. 2001, 106: 381-390.PubMedView ArticleGoogle Scholar
- Nelson G, Chandrashekar J, Hoon MA, Feng L, Zhao G, Ryba NJ, Zuker CS: An amino-acid taste receptor. Nature. 2002, 416: 199-202. 10.1038/nature726.PubMedView ArticleGoogle Scholar
- Li X, Staszewski L, Xu H, Durick K, Zoller M, Adler E: Human receptors for sweet and umami taste. Proc Natl Acad Sci USA. 2002, 99: 4692-4696. 10.1073/pnas.072090199.PubMedPubMed CentralView ArticleGoogle Scholar
- Shi P, Zhang J, Yang H, Zhang YP: Adaptive diversification of bitter taste receptor genes in mammalian evolution. Mol Biol Evol. 2003, 20: 805-814. 10.1093/molbev/msg083.PubMedView ArticleGoogle Scholar
- Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ: Identification and characterization of human taste receptor genes belonging to the TAS2R family. Cytogenet Genome Res. 2002, 98: 45-53. 10.1159/000068546.PubMedView ArticleGoogle Scholar
- Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ: Evolutionary relationships of the Tas2r receptor gene families in mouse and man. Physiol Genomics. 2003, 14: 78-82.View ArticleGoogle Scholar
- Chandrashekar J, Mueller KL, Hoon MA, Adler E, Feng L, Guo W, Zuker CS, Ryba NJ: T2Rs function as bitter taste receptors. Cell. 2000, 100: 703-711.PubMedView ArticleGoogle Scholar
- Bufe B, Hofmann T, Krautwurst D, Raguse JD, Meyerhof W: The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides. Nat Genet. 2002, 32: 397-401. 10.1038/ng1014.PubMedView ArticleGoogle Scholar
- Kim UK, Jorgenson E, Coon H, Leppert M, Risch N, Drayna D: Positional cloning of the human quantitative trait locus underlying taste sensitivity to phenylthiocarbamide. Science. 2003, 299: 1221-1225. 10.1126/science.1080190.PubMedView ArticleGoogle Scholar
- Adler E, Hoon MA, Mueller KL, Chandrashekar J, Ryba NJ, Zuker CS: A novel family of mammalian taste receptors. Cell. 2000, 100: 693-702.PubMedView ArticleGoogle Scholar
- Stevens DR, Seifert R, Bufe B, Muller F, Kremmer E, Gauss R, Meyerhof W, Kaupp UB, Lindemann B: Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli. Nature. 2001, 413: 631-635. 10.1038/35098087.PubMedView ArticleGoogle Scholar
- Ugawa S, Minami Y, Guo W, Saishin Y, Takatsuji K, Yamamoto T, Tohyama M, Shimada S: Receptor that leaves a sour taste in the mouth. Nature. 1998, 395: 555-556. 10.1038/26882.PubMedView ArticleGoogle Scholar
- Dulac C: Sensory coding of pheromone signals in mammals. Curr Opin Neurobiol. 2000, 10: 511-518. 10.1016/S0959-4388(00)00121-5.PubMedView ArticleGoogle Scholar
- Martini S, Silvotti L, Shirazi A, Ryba NJ, Tirindelli R: Co-expression of putative pheromone receptors in the sensory neurons of the vomeronasal organ. J Neurosci. 2001, 21: 843-848.PubMedGoogle Scholar
- Rodriguez I, Del Punta K, Rothman A, Ishii T, Mombaerts P: Multiple new and isolated families within the mouse superfamily of V1r vomeronasal receptors. Nat Neurosci. 2002, 5: 134-140. 10.1038/nn795.PubMedView ArticleGoogle Scholar
- Boschat C, Pelofi C, Randin O, Roppolo D, Luscher C, Broillet MC, Rodriguez I: Pheromone detection mediated by a V1r vomeronasal receptor. Nat Neurosci. 2002, 5: 1261-1262. 10.1038/nn978.PubMedView ArticleGoogle Scholar
- Liman ER, Innan H: Relaxed selective pressure on an essential component of pheromone transduction in primate evolution. Proc Natl Acad Sci USA. 2003, 100: 3328-3332. 10.1073/pnas.0636123100.PubMedPubMed CentralView ArticleGoogle Scholar
- Stowers L, Holy TE, Meister M, Dulac C, Koentges G: Loss of sex discrimination and male-male aggression in mice deficient for TRP2. Science. 2002, 295: 1493-1500. 10.1126/science.1069259.PubMedView ArticleGoogle Scholar
- Leypold BG, Yu CR, Leinders-Zufall T, Kim MM, Zufall F, Axel R: Altered sexual and social behaviors in trp2 mutant mice. Proc Natl Acad Sci USA. 2002, 99: 6376-6381. 10.1073/pnas.082127599.PubMedPubMed CentralView ArticleGoogle Scholar
- Ishii T, Hirota J, Mombaerts P: Combinatorial coexpression of neural and immune multigene families in mouse vomeronasal sensory neurons. Curr Biol. 2003, 13: 394-400. 10.1016/S0960-9822(03)00092-7.PubMedView ArticleGoogle Scholar
- Loconto J, Papes F, Chang E, Stowers L, Jones EP, Takada T, Kumanovics A, Fischer Lindahl K, Dulac C: Functional expression of murine V2R pheromone receptors involves selective association with the M10 and M1 families of MHC class Ib molecules. Cell. 2003, 112: 607-618.PubMedView ArticleGoogle Scholar
- Clyne PJ, Warr CG, Freeman MR, Lessing D, Kim J, Carlson JR: A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila. Neuron. 1999, 22: 327-338.PubMedView ArticleGoogle Scholar
- Vosshall LB, Amrein H, Morozov PS, Rzhetsky A, Axel R: A spatial map of olfactory receptor expression in the Drosophila antenna. Cell. 1999, 96: 725-736.PubMedView ArticleGoogle Scholar
- Gao Q, Chess A: Identification of candidate Drosophila olfactory receptors from genomic DNA sequence. Genomics. 1999, 60: 31-39. 10.1006/geno.1999.5894.PubMedView ArticleGoogle Scholar
- Falk R, Bleiser-Avivi N, Atidia J: Labellar taste organs of Drosophila melanogaster. J Morphol. 1976, 150: 327-342.View ArticleGoogle Scholar
- Dethier VG, Goldrich-Rachman N: Anesthetic stimulation of insect water receptors. Proc Natl Acad Sci USA. 1976, 73: 3315-3319.PubMedPubMed CentralView ArticleGoogle Scholar
- Dethier VG: The physiology and histology of the contact chemoreceptors of the blowfly, Calliphora vicina (insecta, diptera). Quant Rev Biol. 1955, 33: 348-371.View ArticleGoogle Scholar
- Possidente DR, Murphey RK: Genetic control of sexually dimorphic axon morphology in Drosophila sensory neurons. Dev Biol. 1989, 132: 448-457.PubMedView ArticleGoogle Scholar
- Meunier N, Ferveur JF, Marion-Poll F: Sex-specific non-pheromonal taste receptors in Drosophila. Curr Biol. 2000, 10: 1583-1586. 10.1016/S0960-9822(00)00860-5.PubMedView ArticleGoogle Scholar
- Scott K, Brady R, Cravchik A, Morozov P, Rzhetsky A, Zuker C, Axel R: A chemosensory gene family encoding candidate gustatory and olfactory receptors in Drosophila. Cell. 2001, 104: 661-673.PubMedView ArticleGoogle Scholar
- Dunipace L, Meister S, McNealy C, Amrein H: Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system. Curr Biol. 2001, 11: 822-835. 10.1016/S0960-9822(01)00258-5.PubMedView ArticleGoogle Scholar
- Clyne PJ, Warr CG, Carlson JR: Candidate taste receptors in Drosophila. Science. 2000, 287: 1830-1834. 10.1126/science.287.5459.1830.PubMedView ArticleGoogle Scholar
- Brand AH, Perrimon N: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993, 118: 401-415.PubMedGoogle Scholar
- Tanimura T, Isono k, Takamura T, Shimada I: Genetic dimorphism in the taste sensitivity to trehalose in Drosophila melanogaster. J Comp Physiol. 1982, 147: 433-437.View ArticleGoogle Scholar
- Dahanukar A, Foster K, van der Goes van Naters WM, Carlson JR: A Gr receptor is required for response to the sugar trehalose in taste neurons of Drosophila. Nat Neurosci. 2001, 4: 1182-1186. 10.1038/nn765.PubMedView ArticleGoogle Scholar
- Ueno K, Ohta M, Morita H, Mikuni Y, Nakajima S, Yamamoto K, Isono K: Trehalose sensitivity in Drosophila correlates with mutations in and expression of the gustatory receptor gene Gr5a. Curr Biol. 2001, 11: 1451-1455. 10.1016/S0960-9822(01)00450-X.PubMedView ArticleGoogle Scholar
- Wang JW, Wong AM, Flores J, Vosshall LB, Axel R: Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell. 2003, 112: 271-282.PubMedView ArticleGoogle Scholar
- Fiala A, Spall T, Diegelmann S, Eisermann B, Sachse S, Devaud JM, Buchner E, Galizia CG: Genetically expressed cameleon in Drosophila melanogaster is used to visualize olfactory information in projection neurons. Curr Biol. 2002, 12: 1877-1884. 10.1016/S0960-9822(02)01239-3.PubMedView ArticleGoogle Scholar
- Nakai J, Ohkura M, Imoto K: A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat Biotechnol. 2001, 19: 137-141. 10.1038/84397.PubMedView ArticleGoogle Scholar
- Coyne JA, Crittenden AP, Mah K: Genetics of a pheromonal difference contributing to reproductive isolation in Drosophila. Science. 1994, 265: 1461-1464.PubMedView ArticleGoogle Scholar
- Coyne JA, Oyama R: Localization of pheromonal sexual dimorphism in Drosophila melanogaster and its effect on sexual isolation. Proc Nat Acad Sci USA. 1995, 92: 9505-9509.PubMedPubMed CentralView ArticleGoogle Scholar
- Mombaerts P: Molecular biology of odorant receptors in vertebrates. Ann Rev Neurosci. 1999, 22: 487-509. 10.1146/annurev.neuro.22.1.487.PubMedView ArticleGoogle Scholar
- Vosshall LB: The molecular logic of olfaction in Drosophila. Chem Senses. 2001, 26: 207-213. 10.1093/chemse/26.2.207.PubMedView ArticleGoogle Scholar