- Meeting report
- Open Access
Unscrambling the genome
© BioMed Central Ltd 2003
- Published: 30 April 2003
A report on the 24th Annual Lorne Conference on the Organization and Expression of the Genome, Lorne, Victoria, Australia, 16-20 February 2003.
- Nuclear Genome
- Promyelocytic Leukemia
- Transit Peptide
- Cyanobacterial Genome
- Dartmouth Medical School
The recent accumulation of sequence data has allowed more detailed analysis of both protein and RNA products, and their new roles in the function and evolution of organisms and organelles are being revealed. The Lorne Conference on the Organization and Expression of the Genome covered an interesting range of topics - from new functions of RNA to nuclear architecture and genome evolution.
Many people see proteins as more capable and versatile than RNA but recent work suggests new fundamental roles for this nucleic acid. Thomas Gingeras (Affymetrix, Santa Clara, USA) described an array study of the well-characterized human chromosomes 21 and 22 using closely spaced probes to detect expressed sequences. The results are astonishing: more than two thirds of transcripts detected on these chromosomes do not match any known gene and appear to be RNAs that do not encode protein. David Hume (University of Queensland, Brisbane, Australia), who has been working together with the Genome Exploration Research Group at the RIKEN Yokoyama Institute, Kanagawa, Japan, on sequencing full-length murine cDNAs, also reported that around a quarter of expressed mouse sequences appear to be non-coding RNAs. Although these RNAs are sometimes expressed at low levels, the sequence conservation between fish, mice and humans, and the finding that the transcription factors Sp1, Myc and p53 are often found bound to these RNAs' presumed promoters, suggests that the RNAs are genuine products of biological importance.
RNA - the long and the short of it
Although the functions of most non-coding RNAs remain to be defined, there are several examples for which significant progress has been made. Denise Barlow (Institute of Molecular Biology, Salzburg, Austria) explained the role in parent-specific gene silencing (imprinting) of the mouse Igf2r cluster of a 108 kb long non-coding RNA termed Air. Truncating the Air RNA by inserting a polyadenylation and cleavage signal inhibits silencing of the Igf2r locus, suggesting that the RNA itself is critical. The Air RNA, which is encoded within the same gene cluster, is transcribed in the antisense direction relative to the Igf2rgene it silences, but it also turns off other genes within the cluster. Interestingly, it does not overlap with all the genes it affects, arguing against a simple antisense-mediated mechanism that is similar to RNA interference (RNAi).
Among the best understood non-coding RNAs are the microRNAs (miRNAs) that have been characterized by Victor Ambros and colleagues (Dartmouth Medical School, Hanover, USA). The first miRNAs that were identified, lin-4 and let-7, are important developmental regulators in Caenorhabditis elegans. The lin-4 miRNA can base-pair (albeit imperfectly) with seven sites in the 3' untranslated region of its target, the lin-14 mRNA, and inhibits lin-14 translation. The inhibition mechanism is unclear as lin-4 neither causes lin-14 RNA degradation nor prevents loading of the lin-14 mRNA onto ribosomes. Because he noticed that known miRNAs are generated from 70 nucleotide hairpin precursors, Ambros searched the genomes of other organisms for sequences that can form such structures and tested the expression of candidates by northern blotting. Around 120 miRNAs have now been identified in the worm and a similar number in the genomes of higher organisms. The targets of these miRNAs, their mechanisms of action, and the steps in their synthesis are important issues now under investigation.
Short interfering RNAs, the intermediates in the RNAi pathway that mediates post-transcriptional gene silencing, have been used for several years to control gene expression in plants and animals artificially. Peter Waterhouse (Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Canberra, Australia) described his strategy for generating hairpin RNA that is effective in silencing homologous genes in plants. The inclusion of an intron that is spliced out to yield the short hairpin increases efficiency. Waterhouse described his new vectors pHANNIBAL and pHELLSGATE, which can be used in conjunction with recombinase systems in vitro to generate comprehensive libraries of silencing vectors. Steve Whyard (CSIRO Entomology, Canberra, Australia) described how hairpin RNA is being utilized in bio-control to combat insect pests and protect Australia from mollusc species introduced from other countries.
The functions and evolution of conventional organelles, such as chloroplasts, are also becoming increasingly understood. Chloroplasts evolved from proteocyanobacteria. They contain DNA but many genes essential for chloroplast function appear to have moved from the chloroplast into the nuclear genome. How frequently this event occurs was estimated by Chun Huang (University of Adelaide, Australia). A nucleus-specific selectable marker gene (neo) was inserted into the chloroplast genome (plastome) of tobacco plants. Kanamycin-resistant plants, in which the marker gene had moved into the nuclear genome, were obtained at a frequency of 1 in 16,000, suggesting that DNA transfer occurs quite frequently. The work of William Martin (Heinrich-Heine Universität, Düsseldorf, Germany) supports the view that gene transfer from organelles to the nucleus is common. Martin compared the nuclear genome of Arabidopsis with three cyanobacterial genomes and 16 other prokaryotic genomes, in an attempt to estimate how many nuclear Arabidopsis genes originated from the ancestral chloroplasts. The data suggest that around 4,500, or about one fifth of all Arabidopsisgenes, came from chloroplasts. Some, but not all, of these genes encode proteins that are targeted back into the chloroplast and are essential for its function.
The successful migration of a gene from a chloroplast to the nucleus requires not only movement of DNA sequence but also that suitable regulatory elements are present at the new location. But perhaps most interestingly, if the encoded protein is to be targeted back into the chloroplast then it will require the appropriate transit peptide. Geoffrey McFadden (University of Melbourne, Australia) has investigated the amino-terminal sequence extensions required to target proteins into the relict plastids (apicoplasts) of the malarial parasite. The characteristics of the transit peptide had been largely unknown, but McFadden's group has shown that the peptide must be rich in hydrophilic residues, particularly basic residues, and that binding sites for the chaperone protein Hsp70 (DnaK) are important. Bioinformatic searches were then used to identify a number of known and putative nuclearly encoded proteins that are targeted to the apicoplast. This information may help in building a picture of the biology of the malarial apicoplast, and because this plastid is required for the viability of the parasite, agents targeting its function may prove useful in the treatment or prevention of malaria.
In conclusion, the Lorne Genome Conference provides a venue at which data from different organisms are compared. The accumulating information demonstrates not only the complexity of the genome but also its dynamic nature, and takes us one step further towards unscrambling the puzzles of life.