From: New methods for finding disease-susceptibility genes: impact and potential
Attribute type | Attribute | Questions to be asked of each regional gene |
---|---|---|
Transcriptional | Expression state | Which genes are expressed in tissues relevant to the disease of interest? |
 |  | Which genes show restricted or preferential expression in relevant tissues? |
 |  | Which genes show expression within organelles (such as mitochondria) implicated in disease etiology? |
 | Differential expression | Which genes show robust expression differences in comparison of pertinent tissues from 'disease' versus 'control'? |
 |  | Which genes show robust expression differences in response to relevant environmental, pharmacological or physiological manipulation? |
 | Coregulation | Which genes appear to be coregulated with members of other pathways implicated in disease? |
Proteomics | Protein expression | Which genes code for proteins that show differences in expression and/or post-translational modification in diseased tissues, or response to relevant manipulation? |
 | Interaction | Which genes code for proteins known to interact with other proteins implicated in disease etiology? |
 | Function | Which genes code for products that have functional (such as ion channels) or localization (for example, membrane proteins) properties that are pertinent to disease etiology? |
Metabonomics | Biomarkers of disease | Which genes code for proteins involved in regulation of metabolic pathways implicated in disease through metabonomic comparisons? |
 | Pathway analysis | Which genes code for products involved in pathways implicated in disease? |
Comparative analysis | Homologies | Which genes have homologies (or other relationships) to genes implicated in equivalent phenotypes in other animals? |
Ortholog mapping | Â | Which genes are members of gene families for which other members are implicated in the disease or related diseases? |
 |  | Which genes are members of gene families for which other members map to other regions of linkage containing as yet unidentified susceptibility genes? |