- Protein family review
The PITP family of phosphatidylinositol transfer proteins
Genome Biology volume 2, Article number: reviews3011.1 (2001)
The PITP family is one of the two families of phosphoinositide transfer proteins that can bind and exchange one molecule of either phosphatidylinositol or phosphatidylcholine and facilitate the transfer of these lipids between different membrane compartments; the other is the structurally unrelated Sec14p family. PITPs have recently been shown to be critical regulators of phosphoinositides in several cellular compartments, where they participate in signal transduction and in membrane traffic. PITPs were originally defined as soluble, 35 kDa proteins that contain a single structural domain. More recently, however, the PITP domain has also been found in the larger rdgB proteins. Soluble PITPs are found in many organisms, including mammals, Caenorhabditis elegans (worms), Drosophila melanogaster (flies), and Dictyostelium discoideum (slime molds), but not in yeasts or plants. Dysfunction of PITPs leads to neurodegeneration; this highlights the need to understand the biochemical and physiological functions of these proteins in cells.
Two families of proteins are able specifically to transfer phosphatidylinositol (PI) and phosphatidylcholine (PC) in eukaryotic cells (Figure 1), namely the PITP and Sec14p families . They share no obvious sequence or structural similarity, but ectopically expressed PITPs can rescue sec14 mutants in yeast. Likewise, in functional studies in mammalian cells, ectopically expressed Sec14p can be used to compensate for the loss of PITPs [2,3]. Whereas Sec14p isoforms are ubiquitous in eukaryotes, members of the PITP family appear to be absent from plants and fungi. It is likely that endogenous Sec14p and PITP family members normally have distinct biological roles, and the two families should not be confused. Accordingly, PITPs define a discrete family, which forms the subject of this review.
Gene organization and evolutionary history
The first mammalian PITP was identified as a 35 kDa protein with 271 amino acids and no sequence similarity to any known protein . Three subfamilies can now be defined (Figure 2) and all isoforms have an amino-terminal PITP-like domain. Non-systematic nomenclature has arisen as a result of the different methods by which each isoform was identified. All three types occur in humans: the first comprises the small PITPα and PITPβ proteins, which were identified by virtue of their transfer activity in vitro; the second comprises the large rdgBα (also called M-RdgB1, Nir2 and PITPnm) and Nir3 (also called M-RdgB2) proteins; and the third type comprises the rdgBβ protein, which is intermediate in size and was identified by homology to rdgBα . The rdgB acronym is derived from a retinal degeneration mutant phenotype (type B) in Drosophila, and the Nir acronym is derived from a reported interaction with the amino-terminal domain of the Pyk2 tyrosine kinase (Pyk2 N-terminal domain-interacting receptor). A third protein, termed Nir1, was also identified, but this lacks a PITP domain . Mammalian rdgBα is 39% identical in sequence to Drosophila rdgB, and mammalian Nir3 is 46% identical to Drosophila rdgB and 56% identical to mammalian rdgBα .
Intron-exon boundaries are not conserved between different isoforms, and diversity may be enhanced by variation in mRNA splicing . The recent completion of the human genome has revealed that the human PITP chromosomal loci are located at approximately 11q13 (rdgBα), 12q24 (Nir3), 17q13 (PITPα), 17q23-24 (rdgBβ) and 22q12 (PITPβ).
To date, the aforementioned five PITP isoforms have been identified in humans, and one member of each of the three subfamilies can be found in the Drosophila melanogaster genome. Three isoforms can be identified in the genome of C. elegans, including a PITPα or β and a rdgBα or Nir3 homolog; the open reading frame of the third gene is too poorly defined to classify. Two genes have been identified in Dictyostelium discoideum, both of which appear to be PITPα or β homologs . The existence of multiple isoforms within subfamilies appears to be a late event in evolution as, for example, the rat PITPα and PITPβ protein sequences are more similar to each other (77% identity) than to any other PITP. The same is true for murine rdgBα and Nir3 (56% identity) and the Dictyostelium PITP1 and PITP2 sequences (52% identity). Consequently, the different functions of mammalian PITPα and PITPβ will not necessarily parallel the functions of Dictyostelium PITP1 and PITP2; indeed, the subcellular localization of the mammalian and Dictyostelium proteins do not fully correspond .
The absence of PITPs from plants, fungi and bacteria is consistent with a role for these proteins in a subset of phosphoinositide signaling pathways unique to animals. It is unlikely, however, that PITPs are functionally redundant in vivo. For example, reduction of PITPα expression in the vibrator mutant mouse leads to neurodegeneration in the presence of normal, ubiquitous PITPβ expression . Furthermore, although the PITP domain of Drosophila rdgBα can rescue the rdgB-null phenotype, rat PITPα is ineffective . Finally, the as-yet undefined functional properties of at least some PITP isoforms appear to be conserved between species as expression of murine rdgBα fully rescues rdgB-null Drosophila .
Characteristic structural features
The amino acid sequence of the PITP domain is highly conserved in all isoforms and no characteristic short sequence motifs have been identified. (The sequence can be found under the Pfam IDs PF02121 or IP_trans , the PRINTS IDs PR00391 or PITransfer  or the INTERPRO ID IPR001666 ). The only solved crystal structure in this family is of PITPα bound to PC . On the basis of this structure, PITP domains were suggested to comprise three regions (Figure 3a), of which the amino-terminal lipid-binding region contains the most highly conserved residues. This region contains an eight-stranded, concave, mostly antiparallel β-sheet and two helices (Figure 3b), which encircle the bound lipid and are structurally homologous to the START family of lipid-binding proteins . As there is no apparent sequence similarity between PITPs and START proteins, it appears that these families may have evolved convergently. The carboxy-terminal helical region of the PITP domain shows greatest sequence variation and may play an important role in membrane binding. Finally, the intervening loop region, which contains the Ser166 phosphorylation site, has been suggested to mediate several reported protein-protein interactions .
Assignments to the PITPα/β and rdgBβ subfamilies are made according to the similarity of the whole PITP domain rather than any motif. Although human rdgBβ has a short carboxy-terminal extension, the Drosophila isoform does not. In contrast, the rdgBα/Nir3 subfamily is additionally characterized by the presence of an acidic Ca2+-binding domain (Pfam-B_23582 ), six short hydrophobic regions, a putative metal-ion-binding domain (Pfam DDHD ), and a carboxy-terminal Pyk2-binding domain  (Figure 2). The presence of the Pyk2-binding domain in Drosophila rdgB has been noted to require a frame-shift alteration of the previously published sequence . The hydrophobic regions are probably not transmembrane segments as previously proposed , because, although murine rdgBα associates with a particulate brain and retinal fraction on centrifugation, as do other membrane proteins, it can be extracted using buffers containing high salt concentration, high pH or denaturing agents . Sequence features distinguishing individual isoforms in each subfamily remain unclear at present, and may ultimately be better defined by functional information.
Mutation of Thr59, a putative protein kinase C phosphorylation site, in PITPα has been shown to affect PI binding , and this residue is completely conserved in all subfamilies. Modeling the position of PI in the PITPα crystal structure suggests that a hydrogen bond may exist between Thr59 and the inositol residue . The only established post-translational modification, however, is phosphorylation of Ser166 in PITPα by protein kinase C activity. Ser166 is conserved in nearly all PITPs and its phosphorylation has been suggested to affect subcellular localization .
Localization and function
Although PITPs are defined by their ability to bind one molecule of either PI or PC, the affinity of PITPα for PI is 16-fold greater than for PC. This reflects the lower levels of PI than PC in cells, and typically 40% of the PITPα and β proteins are liganded with PC compared to 60% with PI. In addition to their lipid-binding properties, PITPs can transfer PI or PC from one membrane to another down a concentration gradient without input of energy (Figure 1). Thus, PITPs solubilize lipids from membranes and can facilitate their movement through the aqueous phase.
PITPα and PITPβ are expressed ubiquitously in all tissues. In brain, PITPα is very abundant and can represent 0.1% of brain cytosolic protein . During embryonic and early post-natal stages, expression of the genes for both PITPα and PITPβ is detected widely throughout the entire developing central nervous system. In the adult brain, PITPα is expressed in almost all neurons, whereas the expression of PITPβ is lower than at earlier stages in the entire gray matter regions except for the cerebellar cortex . The cellular concentration of PITPα in brain is estimated at 5-10 μM. Most tissues contain both isoforms, but in neutrophils PITPβ is the major isoform and the cellular concentration of PITPβ in these cells is estimated to be between 5 μM and10 μM. Within cells, PITPs are localized in different compartments: PITPα is present in the cytosol and the nucleus, and PITPβ is localized in the Golgi and cytosol but not the nucleus [9,22].
Genetic data indicate that a decrease in PITPα levels leads to neurodegeneration in the mouse . The vibrator mutation appeared spontaneously in three individuals of a litter of nine DBA/2J mice at the Jackson laboratory in 1961. The homozygous vibrator mutation causes an early-onset progressive action tremor, degeneration of neurons in the brain stem and spinal cord, and juvenile death. The mutation has been identified as an intracisternal-A-particle retroposon insertion in intron 4 of the PITPα gene, causing a fivefold reduction in RNA and protein levels. The vibrator phenotype is suppressed in heterozygous mice and in one intercross. The major suppressor locus, termed Modifier of vibrator-1, maps to proximal chromosome 19 but has not yet been identified.
The biochemical function of PITPα has been extensively examined. Several laboratories have used permeabilized cell preparations from which the cytosolic proteins have been depleted. Measurements of phospholipase-C-mediated signaling , regulated exocytosis , the formation of secretory granules  and acid secretion from gastric glands  have been examined, and all these responses were found to be dependent on cytosolic proteins. In all cases, PITPα was purified from brain cytosol as the major reconstituting factor . Several of these functions are summarized in Figure 4. Phospholipase C hydrolyses PI bisphosphate to generate the second messengers (PIP2) diacylglycerol and inositol trisphosphate. Activation of G-protein-coupled receptors or receptor tyrosine kinases is responsible for increasing phospholipase C activity, and PITPα was identified as an essential component in ensuring substrate supply to phospholipase C [28,29]. Analysis of regulated exocytosis was also identified as being dependent on PITPα [18,30,31]. In this case, PITPα functioned together with PI phosphate 5-kinase, indicating that the synthesis of PIP2 was required for the secretory pathway.
Several reports have demonstrated the ability of PITPα to associate with class I and class III PI 3-kinases [32,33] and type II PI 4-kinase  and for rdgBα to associate with type III PI 4-kinase . In addition, rdgBα and Nir3 have been shown to associate with Pyk2, a Ca2+-dependent protein tyrosine kinase . The physiological importance of these different interactions remains unclear, however.
In flies, phototransduction is dependent on a G-protein-coupled phospholipase C signaling pathway and the rdgB protein is essential for phototransduction. The Drosophila rdgB gene was discovered in three independent screens for mutants that exhibited either abnormal photoreceptor physiology or retinal degeneration. The rdgB mutant has a defective light response and its photoreceptors subsequently degenerate, more so in the light. The rdgB gene encodes a 160 kDa protein that is found in the retina, optic lobes, ocelli and in the central brain. In the photoreceptor cell, which consists of an array of densely packed microvilli called the rhabdomere and a non-rhabdomeric cell body, RdgB localizes to the subrhabdomeric cisternae (SRC), which are extensions of the endoplasmic reticulum that act as an intracellular Ca2+ store and also function to deliver protein components and phospholipids such as rhodopsin and PI to the rhabdomere . The SRC and the photoreceptor membrane are in close proximity, separated by a gap of only 10 nm, which may be narrow enough for the transfer of PI between the apposing membranes by the cytoplasmic domain of the rdgB protein .
The mouse and human rdgB homologs are strongly expressed in retina, olfactory bulb and brain and moderately expressed in other tissues, including lung, liver, kidney and spleen [36,37]. Mammalian rdgBα can phenotypically rescue Drosophila rdgB mutants. Human rdgBα maps to chromosome 11q13.1, a region known to contain several retinopathy loci, including Best disease and Bardet-Biedl syndrome-1 [8,37]. Furthermore, in the mouse a dramatic increase in rdgBα expression is seen on the day 17 of gestation, when brain development is at its maximum.
Mammalian Nir3 is selectively expressed in neurons, with high levels in the retina and the dentate gyrus of the hippocampus in the mouse. Unlike rdgBα, Nir3 rescues only photoreceptor degeneration without fully restoring the light response, indicating a functional difference between the two RdgB homologs .
Proteins with a PITP domain are a relatively small family of PI-binding proteins and belong to a larger superfamily of proteins that bind hydrophobic ligands. Despite a wealth of data indicating that PITP proteins play a central role in membrane traffic and signaling, how they execute their function at the molecular level remains to be understood. PITPs appear to coordinate the levels of phosphoinositides in different membrane compartments during periods when high levels of these lipids are needed for both trafficking and signaling purposes, the way the proteins are harnessed in living cells remains to be understood, however.
At the molecular level, understanding of the mechanism by which a PITP molecule abstracts a lipid from a bilayer and facilitates exchange awaits a detailed analysis of the physical properties of the protein and its ability to interact with membranes. A critical point is that PITPs need a sufficiently high affinity for membranes to allow them to release and exchange their bound lipid, but this affinity should be sufficiently low that the protein can move rapidly away from the membrane. Whether lipid composition or curvature of membranes  play roles in modulating this affinity of PITPs for membranes in vivo needs further analysis. Furthermore, how does this biochemical function relate to the requirement for PITPs in membrane budding and fusion? Regulation of these proteins by phosphorylation may provide another important functional determinant. Whether this modification serves as a way of regulating directional transfer of lipids also remains to be studied.
Finally, comprehending the ways in which the regulatory actions of PITPs intertwine with cell signaling and membrane trafficking may provide insights into the neurodegen-eration observed in mouse and fly models that lack PITPs. Here the creation of more refined mouse models with inducible and tissue-specific expression, as well as studies of gene expression during development, will be instrumental in providing insights into the different physiological functions of this versatile protein family.
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Hsuan, J., Cockcroft, S. The PITP family of phosphatidylinositol transfer proteins. Genome Biol 2, reviews3011.1 (2001). https://doi.org/10.1186/gb-2001-2-9-reviews3011
- Retinal Degeneration
- Dictyostelium Discoideum
- Inositol Trisphosphate
- Putative Protein Kinase
- Ser166 Phosphorylation Site