A conserved mechanism for post-transcriptional gene silencing?
Genome Biology volume 1, Article number: reviews1018.1 (2000)
Proteins with homology to RNA-directed RNA polymerases function in post-transcriptional gene silencing: in quelling in the fungus Neurospora crassa, RNAi in the nematode Caenorhabditis elegans, and co-suppression in the mustard plant Arabidopsis thaliana. These findings are consistent with a conserved mechanism operating in these diverse species.
Post-transcriptional gene silencing (PTGS) is a general term for a variety of phenomena that repress gene expression by causing degradation of mRNA. PTGS was discovered 'by accident' in organisms that carried a transgene, were virally infected, or were treated with exogenous RNA. A form of PTGS triggered by transgenic DNA, called co-suppression, was initially described in plants [1,2], and a related phenomenon, termed quelling, was later observed in the filamentous fungus Neurospora crassa . Co-suppression was first noticed when a transgenic petunia, expected to express a transgene involved in pigment formation at a high level, instead expressed neither the transgene nor related, endogenous genes [1,2]. Subsequent work indicated that viral infection can also trigger co-suppression in plants , leading to the hypothesis that the biological role of PTGS is as an anti-viral defense mechanism. Meanwhile, other experiments with the soil nematode Caenorhabditis elegans uncovered a phenomenon triggered by double stranded (ds) RNA, called RNA interference (RNAi), as well as transgene-induced co-suppression [5,6]. RNAi is an extremely valuable tool for 'reverse' genetic studies because it gives researchers a quick means to determine the loss-of-function phenotype for a gene. A wide variety of organisms have now been shown to respond to RNAi, including Drosophila and mouse (see, for example, [7,8,9]).
Because of the utility of PTGS for reverse genetic studies and its possible clinical uses, there is a great deal of interest in the underlying molecular mechanism(s). It has become apparent that different PTGS phenomena have common characteristics, and this realization has led to the speculation that PTGS in different organisms may be mediated by similar molecular mechanisms [6,10,11]. Recent genetic and molecular studies provide additional support for this hypothesis. Genes involved in PTGS have been identified in N. crassa (qde genes) , C. elegans (rde, mut, and ego-1 genes) [13,14,15], and the mustard plant Arabidopsis thaliana (sde, sgs genes) [16,17,18]. Intriguingly, related proteins function in PTGS in these organisms (Table 1), as would be expected if different forms of PTGS occur by similar mechanisms.
Among proteins associated with PTGS to date, the most widely conserved are those with homology to tomato RNA-directed RNA polymerase (RdRP) : Neurospora QDE-1 , C. elegans EGO-1 , and Arabidopsis SGS-2/SDE-1 ([17,18]; Figure 1). The recent addition of Arabidopsis genes to this collection suggests that PTGS is a widely conserved, evolutionarily ancient means of gene regulation. Two additional protein families have been linked to PTGS in nematodes and Neurospora (Table 1) [13,14,21,22] but such a link has yet to be made in plants. The rde-1 and qde-2 genes are members of the piwi/sting family. Piwi/Sting proteins are related to eIF2C, a proposed translation factor (see [13,21]); several members of this family are known to have important developmental functions. The mut-7 and qde-3 genes encode members of the WRN (Werner's syndrome) protein family, whose members include several RecQ DNA helicases; Werner's syndrome is associated with premature aging. QDE-3 and MUT-7 are predicted to have nucleic acid binding activity: QDE-3 has strong homology to the DNA helicase domains  and MUT-7 has homology to the RNase catalytic domains .
Independent screens by the Vaucheret and Baulcombe laboratories for co-suppression-defective mutants in Arabidopsis have recovered mutations in a RdRP-related gene, named sgs2 (suppressor of gene silencing)  or sde1 (silencing defective) . For the purposes of this review, the gene is referred to as sgs2/sde1. Mutants were isolated by screening for individuals defective in transgene-induced PTGS. Several other RdRP-related genes exist in Arabidopsis, but no mutations were recovered in those genes.
Because co-suppression in plants can be triggered by viral infection and, in fact, is hypothesized to be a viral defense mechanism, both groups investigated the effects of viral infection on sgs2/sde1 mutants. Was virally induced PTGS defective? Were the mutants super-susceptible to viral infection? Dalmay and colleagues  found that PTGS triggered by tobacco mosaic virus and tobacco rattle virus is not defective in sde1 mutants. They interpret their data as meaning that SDE1 is not required for virally induced PTGS, but only for transgene-induced PTGS. They suggest that virally encoded RdRP activity can substitute for the putative RdRP activity of SDE1. In contrast, Mourrain and colleagues  found that sgs2 mutants had increased susceptibility to infection by certain viruses, such as a cucumovirus, but not others, such as a tobamovirus or a potyvirus. They interpret these results as indicating that PTGS indeed acts as a defense against at least some viruses. Interpretation of infection data is complicated by the fact that many viruses can inhibit PTGS: tobamovirus and potyvirus strongly inhibit PTGS, whereas cucumovirus inhibits it only weakly [17,23]. Logically, the loss of a plant component of PTGS, such as SGS2/SDE1, will have no effect on a virus that fully blocks PTGS by itself, but would allow a higher level of infection by a virus that disables PTGS only partially or not at all. At any rate, these results suggest that the Dalmay et al. model is unlikely to be correct for all viruses; in at least some cases, viral RdRP cannot replace SGS2/SDE1.
The RdRP protein family
RdRP activity has been known in plants for decades, although its physiological function has been unclear . Many models for PTGS in plants postulate an RNA amplification step that could be accomplished by RdRP activity (for example in ), but it has yet to be shown definitively that RdRP is in fact involved in PTGS. In light of these models, it is intriguing that proteins related to the purified tomato RdRP have now been linked to PTGS in diverse organisms. It should be noted, however, that these proteins have not yet been shown to have RdRP activity. They contain large regions of sequence conservation with tomato RdRP, which may encode RdRP activity, but they also contain extensive regions of divergent sequences (see Figure 1) [15,17,18].
Analysis of genome sequence data has shown that C. elegans and Arabidopsis contain several RdRP-related genes. In C. elegans, for example, there are three rrf (RdRP family) genes in addition to ego-1. Thus far, six other RdRP-related genes have been found in the Arabidopsis genome in addition to sgs2/sde1. Genome sequencing has uncovered RdRP-related genes in many other organisms, including several plant species, such as wheat and petunia, and the fission yeast Schizosaccharomyces pombe. It is not yet clear though how many of these RdRP-related proteins actually function in PTGS. Some of them may have other cellular functions, perhaps in RNA metabolism. Indeed, ego-1 was identified originally as being important in development of the C. elegans germ line, and was only later shown to function in RNAi; the connection between development and RNAi is not yet clear, but one possibility is that EGO-1 protein has different functions in the two processes. Since alleles of only one RdRP-related gene, sgs2/sde1, were recovered as suppressors of PTGS, perhaps the related genes in Arabidopsis do not function in PTGS. Alternatively, some or all of these genes may be redundant, so that simultaneous mutations in two or more of them would have to be induced in order to see a phenotype. In addition, if any RdRP-related gene is essential, it would not have been identified because the screens for silencing-defective mutants did not allow for recovery of lethal mutations.
RdRP-related genes are apparently absent from at least one organism that is susceptible to RNAi: Drosophila melanogaster. Genome sequence data are nearly complete for Drosophila, yet no gene with homology to either tomato or viral RdRP has been found. Since viral and cellular (for example, tomato) RdRP have little amino acid homology to each other, perhaps RdRP function in Drosophila is accomplished by an enzyme with yet a different amino acid sequence. Alternatively, RNAi in Drosophila may not require RdRP activity at all.
Molecular characterization of another Arabidopsis sgs gene, sgs3, by Mourrain et al.  revealed that it encodes a novel protein. In general, sgs3 mutants behave like sgs2 mutants, including having an increased susceptibility to cucomovirus infection. Thus, it appears that SGS3 is as critical to PTGS as is SGS2/SDE1. No SGS3 relative has been found yet in any other organism, including those with fully sequenced genomes such as C. elegans and Drosophila and this result is consistent with SGS3 functioning in a plant-specific aspect of PTGS.
The extensive and growing genome sequence database is an important resource for studying the mechanics of PTGS and the role of RdRP-related genes in this and other cellular processes. Three protein families are now known to be important to PTGS. Genome sequence information has allowed researchers to identify paralogs and orthologs very quickly, and provides a focus for future experiments. For example, reverse genetic approaches can be used to generate deletion mutations in these related genes for use in studying their possible role in PTGS. We can expect a more complete molecular portrait of PTGS to be revealed in the near future.
Napoli C, Lemieux C, Jorgensen R: Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous gene in trans. Plant Cell. 1990, 2: 279-289. 10.1105/tpc.2.4.279.
van der Krol AR, Mur LA, Beld M, Mol JNM, Stuitje AR: Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell. 1990, 2: 291-299. 10.1105/tpc.2.4.291.
Romano N, Macino G: Quelling: transient inactivation of gene expression in Neurospora crassa by transformation with homologous sequences. Mol Microbiol. 1992, 6: 3343-3353.
Ruiz MT, Voinnet O, Baulcombe DC: Initiation and maintenance of virus-induced gene silencing. Plant Cell. 1998, 10: 937-946. 10.1105/tpc.10.6.937.
Guo S, Kemphues KJ: par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell. 1995, 81: 611-620.
Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998, 391: 806-811. 10.1038/35888.
Kennerdell JR, Carthew RW: Use of ds-RNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the Wingless pathway. Cell. 1998, 95: 1017-1026.
Fortier E, Belote JM: Temperature-dependent gene silencing by an expressed inverted repeat in Drosophila. Genesis. 2000, 26: 240-244. 10.1002/(SICI)1526-968X(200004)26:4<240::AID-GENE40>3.0.CO;2-P.
Wianny F, Sernicka-Goetz MW: Specific interference with gene function by double-stranded RNA in early mouse development. Nat Cell Biol. 2000, 2: 70-75. 10.1038/35000016.
Montgomery MK, Fire A: Double-stranded RNA as a mediator in sequence-specific genetic silencing and co-suppression. Trends Genet. 1998, 14: 255-258. 10.1016/S0168-9525(98)01510-8.
Fire A: RNA-triggered gene silencing. Trends Genet . 1999, 15: 358-363. 10.1016/S0168-9525(99)01818-1.
Cogoni C, Macino G: Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing in Neurospora crassa. Proc Natl Acad Sci USA. 1997, 94: 10233-10238. 10.1073/pnas.94.19.10233.
Tabara H, Sarkissian M, Kelly WG, Fleenor J, Grishok A, Timmons L, Fire A, Mello CC: The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell. 1999, 99: 123-132.
Ketting RF, Haverkamp THA, van Luenen HGAM, Plasterk RHA: mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell. 1999, 99: 133-141.
Smardon A, Spoerke JM, Stacey SC, Klein ME, Mackin N, Maine EM: EGO-1 is related to RNA-directed RNA polymerase and functions in germline development and RNA interference in C. elegans. Curr Biol. 2000, 10: 169-178. 10.1016/S0960-9822(00)00323-7.
Elmayan T, Balzergue S, Beon F, Bourdon V, Daubremet J, Guenet Y, Mourrain P, Palauqui JC, Vernhettes S, Vialle T, et al: Arabidopsis mutants impaired in cosuppression. Plant Cell. 1998, 10: 1747-1758. 10.1105/tpc.10.10.1747.
Mourrain P, Beclin C, Elmayan T, Feuerbach F, Godon D, Morel JB, Jouette D, Lacombe AM, Nikic S, Picault N, et al: Arabidopsis SGS2 and SGS3 are required for posttranscriptional gene silencing and natural virus resistance. Cell. 2000, 101: 533-542.
Dalmay T, Hamilton A, Rudd S, Angell S, Baulcombe D: An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell. 2000, 101: 543-553.
Schiebel W, Pelissier T, Riedel L, Thalmeir S, Schiebel R, Kempe D, Lottspeich F, Sanger HL, Wassenegger M: Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato. Plant Cell. 1998, 10: 2087-2102. 10.1105/tpc.10.12.2087.
Cogoni C, Macino G: Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase. Nature. 1999, 399: 166-169. 10.1038/20215.
Catalanotto C, Assalin G, Macino G, Cogoni C: Gene silencing in worms and fungi. Nature. 2000, 404: 245-10.1016/S0014-5793(97)00089-6.
Cogoni C, Macino G: Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase. Science. 1999, 286: 2342-2344. 10.1126/science.286.5448.2342.
Voinnet O, Pinto YM, Baulcombe D: Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc Natl Acad Sci USA. 1999, 24: 14147-14152. 10.1073/pnas.96.24.14147.
Astier-Manifacier S, Cornuet P: RNA-dependant RNA polymerase in Chinese Cabbage. Biochim Biophys Acta. 1971, 232: 484-493. 10.1016/0005-2787(71)90602-2.
Wassenegger M, Pelissier T: A model for RNA-mediated gene silencing in higher plants. Plant Mol Biol. 1998, 37: 349-362. 10.1023/A:1005946720438.
The author thanks John Belote and M Kathryn Barton for helpful comments on the manuscript.
About this article
Cite this article
Maine, E.M. A conserved mechanism for post-transcriptional gene silencing?. Genome Biol 1, reviews1018.1 (2000). https://doi.org/10.1186/gb-2000-1-3-reviews1018
- Nematode Caenorhabditis Elegans
- RdRP Activity
- Viral RdRP
- Nucleic Acid Binding Activity
- Soil Nematode Caenorhabditis Elegans