Fig. 4
From: Enhancing prime editor flexibility with coiled-coil heterodimers
Prime editing using NG-CC-PE or SpRY-CC-PE system. a Precise editing and undesired indel efficiencies of NG-sPE-P3P4, NG-sPE-N5N6, NG-sPE-ctrl, and NG-PE2 at FANCF (+ 1 G deletion), MECP2 (+ 1 C deletion), VEGFA (+ 1 C to A), MECP2 (+ 1 C to A), VEGFA (+ 1 T insertion), and MECP2 (+ 1 A insertion) loci in HEK293T cells. b Precise editing and undesired indel efficiencies of SpRY-sPE-P3P4, SpRY-sPE-N5N6, SpRY-sPE-ctrl, and SpRY-PE2 at DYRK1A (+ 5 A deletion), CUL3 (+ 12 ~ 13 CA deletion), MSTN (+ 1 G deletion), DYRK1A (+ 9 T to A), CUL3 (+ 1 A to G), and CUL3 (+ 8 C insertion) loci in HEK293T cells. Data in a and b were obtained from three or more independent biological replicates (mean ± s.d.) via unpaired t-tests (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns indicating not significant)