Table 4 Antibody and reporter cycle layout, including LED intensities and exposure times for each marker
From: FixNCut: single-cell genomics through reversible tissue fixation and dissociation
Cycle # | Cy3 (LED 100%) | Exposure time (ms) | Cy5 (LED 100%) | Exposure time (ms) | Cy7 (LED 60%) | Exposure time (ms) |
|---|---|---|---|---|---|---|
1 | BLANK | 250 | BLANK | 350 | BLANK | 500 |
2 | e-cadherin-RX014 | 750 | ERG-RX031 | 850 | SMA-RX013 | 500 |
3 | CD8-RX026 | 750 | CD3e-RX045 | 850 | PanCK-RX019 | 400 |
4 | Empty | 250 | p63-RX046 | 700 | Empty | 500 |
5 | BLANK | 250 | BLANK | 350 | BLANK | 500 |