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Table 3 Flow cytometry lipid-modified oligos (LMOs)

From: FixNCut: single-cell genomics through reversible tissue fixation and dissociation

Reagent

Sequence

Company

LMO001A Lignoceric Anchor with DNA Oligo

Commercial source

Sigma-Aldrich

LMO001B Palmitic Co-anchor with DNA Oligo

Commercial source

Sigma-Aldrich

A647_Oligos

5′-CCTTGGCACCCGAGAATTCCA-A_647-3 (complementary to the Anchor) or /5Alex647N/CCTTAGCCGCTAATAGGTGAGC (Complementary to Capture sequence of Barcoded Oligo CCTTGGCACCCGAGAATTCCA[Barcode]GCTCACCTATTAGCGGCTAAGG)

Oligo from IDT