Table 2 Flow cytometry antibodies and reagents
From: FixNCut: single-cell genomics through reversible tissue fixation and dissociation
Antibody | Clone | Conjugate | Company |
|---|---|---|---|
Human BD Fc Block | Fc1 | NA | BD # 564219 |
Anti-human CD45 | HI30 | BV510 | BD # 563204 |
HI30 | PE | BD Pharmingen | |
Anti-human CD3 | UCHT1 | BV711 | BD # 563725 |
SK7 | PE-Cy7 | Biolegend PN-344816 | |
OKT3 | PerCP | Biolegend | |
Anti-human CD4 | SK3 | BV650 | BD # 563875 |
RPA-T4 | Alexa Fluor® 488 | Biolegend PN-300519 | |
Anti-human CD8 | HIT8a | PE | BD #555635 |
SK1 | APC-Cy7 | Biolegend PN-344714 | |
Anti-human CD19 | 6E10 | Alexa Fluor® 647 | Biolegend PN-302222 |
Anti-human CD298 | LNH-94 | PE-Cy7 | BioLegend #341707 |
Anti-human β2-microglobulin | 2M2 | APC | BioLegend #316311 |
Anti-human CD11b | ICRF44 | PeCy7 | Biolegend |
Anti-human EPCAM | 9C4 | APC | Biolegend |
Annexin V | FITC | BD #556420 | |
Propidium Iodine | Sigma-Aldrich #P4864 |