Fig. 5

Minimization of technical artifacts using FixNCut protocol on mouse tissues. This figure shows the impact of various dissociation-induced gene signature scores, including dissociation on mouse muscle stem cells [22], warm dissociation on mouse kidney samples [3], and warm collagenase dissociation on mouse primary tumors and patient-derived mouse xenografts [2], across mouse tissues and processing protocols used. All statistical analyses between protocols were performed using the Wilcoxon signed-rank test; significance results are indicated with the adjusted p-value, either with real value or approximate result (ns, p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001). a Violin plots of dissociation-induced gene signatures scores for fresh and fixed mouse lung. b Score of warm collagenase gene signature for fresh and fixed mouse lung samples across cell populations. c Overlap of differentially expressed genes in the fresh and fixed mouse lung sample with genes from the three dissociation-induced signatures. d Violin plots of dissociation-induced gene signatures scores for fresh and fixed mouse colon. e Score of warm collagenase gene signature for fresh and fixed mouse colon samples across cell populations. f Overlap of differentially expressed genes in the fresh and fixed mouse colon sample with genes from the three dissociation-induced signatures. g Violin plots of dissociation-induced gene signatures scores for cryo and fixed/cryo mouse lung. h Score of warm collagenase gene signature for cryo and fixed/cryo mouse lung samples across cell populations. i Overlap of differentially expressed genes in the cryopreserved and Fixed/Cryo mouse lung sample with genes from the three dissociation-induced signatures