Fig. 8
From: Liquid–liquid phase separation of H3K27me3 reader BP1 regulates transcriptional repression
Phase separation of BP1 orthologs is ubiquitous in fungi. A Venn diagram of IDR distribution in BP1 orthologs from fungi (407 total, black). Among the fungal BP1 orthologs, the number of proteins containing IDR1 (327, green) and IDR2 (373, blue) is shown. B, C The IDRs of Magnaporthe oryzae MGG_09903 (B) and Neurospora crassa NCU07505 (C) were detected by the Predictor of Natural Disordered Regions database. D Coomassie brilliant blue staining of recombinant His-MGG_09903-GFP and His-NCU07505-GFP proteins purified from E. coli. E Turbidity assay of recombinant His-MGG_09903-GFP and His-NCU07505-GFP in buffer containing 10% (w/v) PEG 8000 to visualize separation of high-concentration condensates. F Representative micrographs of His-MGG_09903-GFP and His-NCU07505-GFP protein preparations. G, H Quantifications of droplet numbers (G) and droplet area (H) are shown