Fig. 7

Satellite and chromatin organization at centromere junctions with pericentric and telomeric sides. Alignments of A MiSat and MaSat reference consensus sequences with repeat units from representative MiSat-MaSat junction containing arrays. B TLC Sat, MiSat, and telomeric repeat consensus with repeat units from representative Cen-Tel junction arrays. Reference satellite consensus sequences used for the alignments are as described previously [47, 48, 51]. The length of each array is given, and the X-axis is not to the scale. All subunits are arranged in the order they appear, spanning from the beginning to the end of a given array. The alignment of all ordered repeat units with the reference consensus is performed for the entire array. Alignments of repeat units from the beginning of a given array are shown. C CENP-A, H3K9me3, and H3K27me3 chromatin enrichment on MiSat-MaSat and MiSat-TLC Sat junctions calculated by normalizing the ChIP enrichment with the abundance in the ChIP input of the respective MiSat, MaSat or TLC Sat containing part of the junction array. The normalized enrichment value was averaged over three or more arrays for each type