Fig. 6

Continuous expression of A3B is required for RNA editing. A Schematic representation of the strategy used to study whether A3B expression is needed to detect the RNA edits. B, C and D Examples of Sanger sequencing chromatograms from the livers for the A3B-driven edited positions. B Mice 4 days after dox administration. Control refers to a mouse that has the TetO-A3B transgene but not the CAGs-rtTA3, while A3B is a mouse with TetO-A3B/CAGs-rtTA3 genotype. C Mice on dox for 4 days and placed back on a normal diet for 12 days or off dox for 16 days. D Mice that received a pulse of A3B expression (4 days dox and up to a year on normal diet) or expressing A3B in a cycle manner (4 days dox-26 days off dox/monthly). Samples were collected at experimental endpoint (1 year). E Schematic of the breeding strategy to obtain TetO-A3B-^E255A/CAGs-rtTA3 mice. F Immunohistochemistry of tGFP in the indicated tissues from A3B-^E255A fed with dox for 8 days. Scale bar: 100 μm. G Deamination activity assay in the indicated tissues from TetO-A3B-^E255A/CAGs-rtTA3 mice fed with dox for 8 days (S, Substrate; P, Product). H Examples of Sanger sequencing chromatograms showing no RNA editing in A3B-^E255A liver tissues