Fig. 3

Local preference of APOBEC3B-driven RNA editing. A Schematic of the pipeline used to call RNA-editing sites in the A3B livers and pancreas. We used Mutect2 to identify DNA mutations and RNA edits. We pooled the WES and RNA-seq of the two control animals (no dox), and used them as a reference to identify variants of the A3B expressing tissues. This step was performed separately for DNA and RNA sequences, leading to the identification of DNA variants or RNA variants that were detected in at least one A3B expressing tissues but not in any of the other two control tissues. After that, we compared the RNA variants with the DNA variants from each sample to identify the RNA edits that are not DNA SNPs. B Trinucleotide mutation profiles for all base substitutions in the RNA from liver and pancreatic tissues of A3B mice (n = 6 in each group). Relative contribution refers to the contribution that each single base substitution has to the overall base substitution spectrum in A3B mice. C-D Lollipop plots indicating the percentage of mice showing C-to-U editing after experimental validation by RT-PCR in selected targets (n = 6 tissues in each group). E–F Heatmap plot showing the editing ratios of each sanger sequence validated position in each individual sample (liver shown in E and pancreas in F)