Fig. 1

Generation of A3B-inducible mice. A Schematic representation of the strategy used for the generation of human A3B transgenic mice. Under a TRE promoter, the human A3B cDNA fused to tGFP was inserted after homologous recombination into the ColA1 locus of KH2 ES cells. B Macroscopic images demonstrate tGFP fluorescence in tissues from A3B mice fed with doxycycline for 10 days (scale bar: 3 mm). C Immunohistochemistry of A3B in the indicated tissues from A3B mice fed with doxycycline for 10 days (scale bar: 100 μm). D Western blot analysis showing A3B levels in the indicated tissues from A3B mice with and without doxycycline treatment for 10 days. Anti-actin blots are shown as loading controls. E Representative DNA cytosine deaminase activity from whole cell extracts in the indicated tissues (S, Substrate; P, Product) and the corresponding quantification showing the % of the deaminated oligo (n = 5 mice per tissue)