Fig. 1

Archaeal ATAC-seq and the open chromatin landscape of H. volcanii. A, B Adaptation and optimization of the ATAC-seq assay to the archaeal context. A Distribution of TSS ratio scores (see the “Methods” section for details) for native, 0.1%- and 1%-formaldehyde ATAC-seq libraries. C Fragment length distribution in H. volcanii ATAC-seq datasets. D Estimated relative copy number of H. volcanii chromosomes. Genomic DNA was tagmented and amplified (n = 4) and normalized read coverage was estimated for each chromosome/plasmid. The average ratios are shown. E Distribution of MACS2 ATAC-seq peaks relative to TSSs. F, G Representative browser snapshots of ATAC-seq profiles along the H. volcanii genome. F High reproducibility of H. volcanii chromatin accessibility measurements using ATAC-seq. Shown is the between-replicate correlation over TSSs in RPM (reads per million) units. I Global ATAC-seq profile over each of the five H. volcanii chromosomes. The number in brackets corresponds to the magnification of the true proportional size of plasmids relative to the main chromosome