Fig. 3
Intracellular lipid accumulation increases loss of expression and transcriptional variability. A Box plots depicting the coefficient of variation per gene in cells treated with DMSO or with FFA per subgroup (* = p-value < 0.05, Mann–Whitney U). B Bar plot showing the percentage of cells treated with DMSO or with FFA in every subgroup. C Stacked violin plots of the genes used in Fig. 1 to identify the subgroups, split into DMSO- and FFA-treated cells (top) and top 5 up-regulated genes upon fat accumulation per subgroup (bottom) in DMSO and FFA treatments (* = p-value < 0.05 and |log2-fold change|> 0.75, t-test). D Heatmap depicting the logarithmic mean expression of genes related to lipid metabolism, lipid storage, NAFLD-related genes, inflammation, and stress response in DMSO- and FFA-treated cells per subgroup (↑ indicates up-regulation towards DMSO; ↓ indicates down-regulation towards DMSO, t-test). E Scatter plot of the gene ontology (GO) analyses using gprofiler of the genes up-regulated upon fat accumulation in each of the subgroups. The top 7 most significantly enriched terms are depicted