Fig. 4

GoM DE analysis vs. DESeq2 analysis in PBMC data. A and B compare differential expression in topics 1 and 2 (Fig. 1) with their closely corresponding FACS cell populations. Genes are only shown if the posterior z-score was greater than 2 in magnitude in at least one of the DE analyses. Genes are colored by the “null model” expression rate. The Q-Q plot (C) compares the overall distribution of posterior z-scores for B cells and myeloid cells (x-axis) and for topics 1 and 2 (y-axis). For better visualization of quantiles near zero, posterior z-scores larger than 20 in magnitude are shown as 20 or −20. Analysis of differential expression among the 6 FACS cell populations was performed using DESeq2 [79, 84]