Fig. 5

Expression pattern and subcellular localization ofTaSPL17. a RT-PCR analysis showing the relative transcript levels of TaSPL17-A, TaSPL17-B, and TaSPL17-D in various organs (root, shoot, leaf blade, young and mature stems, and inflorescence at different developmental stages). The wheat Actin gene was used as an internal control. Values are shown as means ± SD of three independent experiments and three biological replicates. b–f mRNA in situ hybridization reveals that TaSPL17 is preferentially expressed in young leaves (YL), tiller buds (TB), root meristems (RM), the shoot apex meristem (SAM), spikelet meristems (SM), floret meristems (FM), and florets (FL). Arrows indicate the locations where TaSPL17 is expressed. g TaSPL17 sense probe was used as a negative control. h Subcellular localization of TaSPL17 encoded by each subgenome. A construct encoding TaSPL17-A-GFP, TaSPL17-B-GFP, or TaSPL17-D-GFP was co-expressed with OsMADS3-mCherry, encoding a nuclear marker. From left to right: image of GFP (green), mCherry (red), protoplast, and merged GFP and mCherry. Scale bars, 200 μm in b-e, 500 μm in f, g, or 20 μm in h