Fig. 7

PMDs and hypoDMRs exhibit strong cell-type-specific epigenomic features. A Line plots showing average methylation levels for different PMD or C hypoDMR categories comparing two types of nonmalignant esophageal samples; these changes in nonmalignant samples are similar to those seen in tumors (Fig. 2C, Additional file 1: Fig. S4D-E). B Volcano plots showing average methylation levels for different PMD or D hypoDMR categories in nonmalignant esophageal samples. Regions with significant differences were determined by two-tailed t test with the FDR cutoff < 0.1. E UMAP plots showing cell clusters (left), ATAC-seq levels in ESCC- vs. EAC-specific PMDs (middle) or in ESCC- vs. EAC-specific hypoDMRs (right). Single-cell ATAC-seq values and the cluster scheme were from Zhang et al. Total cell number is 146,305. F, G Dot plots showing, at the sample level, delta ATAC-seq values in ESCC- vs. EAC-specific PMDs (F) or in ESCC- vs. EAC-specific hypoDMRs (G)