Table 5 Comparison of 10x vs. TELL-Seq de novo assembly statistics as calculated by MetaQUAST. MOCK20 datasets were made using 10x and TELL-seq library preparation and sequencing protocols, reference-deconvolved, and de novo assembled using cloudSPAdes
From: Ariadne: synthetic long read deconvolution using assembly graphs
Summary statistic | MOCK20 10x | MOCK20 TELL-Seq |
|---|---|---|
Genome fraction (%) | 75.827 | 67.719 |
Total aligned length (bp) | 52,363,841 | 45,407,218 |
Longest single-contig alignments (bp) | 6,234,231 | 2,079,899 |
Num. contigs | 2481 | 7729 |
Num. misassemblies | 127 | 59 |
Total size of misassembled contigs (bp) | 23,155,091 | 15,651,764 |
Proportion of assembled sequence in misassembled contigs | 44.22% | 34.47% |
Num. unaligned contigs | 1 | 45 |
Size of unaligned contigs (bp) | 599 | 105,526 |