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Fig. 3 | Genome Biology

Fig. 3

From: LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis

Fig. 3

Characterization of transcriptional bursting kinetics in human cells by LAST-seq. a Technical noise plotted by the distribution of coefficient of variation (CV) of each mRNA species from 10 samples (LAST-seq) or 16 samples (SMART-seq). b Workflow of single-cell transcriptome analyses by LAST-seq to minimize the extrinsic noise. c Gene expression noise profile of haploid human cells. The red line represents the theoretical level of technical noise. d Two-state model of transcriptional bursting. e Examples of curve fitting to derive transcriptional burst parameters from the intrinsic noise of gene expression. f Transcriptome-wide distribution of burst frequency and burst size for human genes. g Correlation between the expression noise and burst frequency. h Correlation between the expression level and burst size. i Distribution of burst frequency and burst size for human genes located in A (n = 3465) and B (n = 645) compartments. The statistical test of (a) and (i) was performed by two-sided Kolmogorov–Smirnov test (**p ≤ 0.01, ****p ≤ 0.0001)

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Genome Biology

ISSN: 1474-760X

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