Fig. 2

Comparison between LAST-seq and CEL-seq. a Experimental schematic to compare LAST-seq and CEL-seq in detecting RNA spike-in species and transcriptomic RNA molecules. b Capture efficiency of LAST-seq and CEL-seq using ERCC RNA spike-in as the input, with dots representing the average number of detected UMIs from 10 and 5 replicates for LAST-seq and CEL-seq, respectively. c Capture efficiency of LAST-seq and CEL-seq using A60 RNA spike-in as the input, with dots representing the average number of detected UMIs from 10 and 5 replicates for LAST-seq and CEL-seq, respectively. d Correlation of RNA level quantified by UMI numbers between LAST-seq and CEL-seq, both averaged from 10 single cells. Each dot corresponds to one gene. e Number of UMIs per gene comparison under various sequencing depths between LAST-seq and CEL-seq, both averaged from 10 single cells. f Number of total UMI per cell comparison under various sequencing depths between LAST-seq and CEL-seq, both averaged from 10 single cells. The boxplot shows the median (center line), the 25/75 percentile (bounds), and the minimum/maximum (whiskers). The statistical analysis was performed by the Welch t-test (**p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001)