Fig. 1

Human and chimpanzee embryoid bodies are composed of 86 cell types from all three germ layers. A Overview of experimental design. EBs were generated in three replicates of six to nine human and chimpanzee iPSC lines. After two days in micropatterned plates, EBs were grown in low-adherence plates for three weeks before dissociating to single cells and sequencing with 10x scRNA-seq. B UMAP projection of all human and chimpanzee single cells after reference alignment. Cells are colored and labeled with the reference-assigned cell type. C The UMAP in B colored by germ layer of each cell type. The UMAP in B with only D human or E chimpanzee cells