Fig. 4

The SNORD2-intron structure is correlated with the splicing level of the exon 4 of EIF4A2. A SNORD2-intron is detected in RNA-seq (TGIRT-Seq) in normal human tissues and in human cell lines. Bedgraphs of RNA-Seq read profiles of the EIF4A2 exon 3–4 genomic region from normal human tissues and human cell lines show the presence of accumulation in the intronic SNORD2 interaction region (in cyan in schema at top). Red arrows show the tissues or cell lines having clear read accumulations in the intron target region. B Correlation between mature SNORD2 and EIF4A2 abundance. Scatterplot showing the abundance of the mature form of SNORD2 and of the total transcript level of EIF4A2 in the indicated tissues and cell lines. A light non-significant negative correlation was found between the level of abundance of SNORD2 compared to the level of abundance of EIF4A2 gene. C No correlation was found between the abundance of mature SNORD2 and the splicing of EIF4A2 exon 4. PSI: percent spliced in. D A significant negative correlation was found between the abundance of the SNORD2 extension and the splicing of EIF4A2 exon 4