Fig. 7
Protein dynamics could be predicted by RPF dynamics plus the initial protein abundance. A The kinetic models used for protein dynamics prediction. In the RPF-only model, protein abundance is determined by protein synthesis and degradation. In the \({P}_{0}\)+ RPF model, the initial protein level (P0) is additionally considered. α, constant from translation to protein; \({k}_{d}\), degradation rate; \({RPF}_{t}\): translation level at time t; \({P}_{t}\): protein abundance at time t. B Heat maps showing the observed protein and RPF levels with the corresponding predicted protein dynamics using RPF-only and \({P}_{0}\)+ RPF models mapped. Red boxes indicate stages that show differential signals between observed protein and RPF. C Dot-line plots showing the protein levels predicted by the \({P}_{0}\)+ RPF model (line) and observed from LC–MS/MS (dot) for representative genes (right). RPF level at each stage is also shown (left). D Histogram showing the Spearman correlation coefficients between observed protein abundance and RPF level (blue) or predicted protein levels by RPF-only (green) and \({P}_{0}\)+RPF models (red). E Summary model of the protein and RPF dynamics in mouse oocytes and early embryos