Fig. 4

Correlation among protein, RPF, and mRNA during oocyte maturation and early embryo development. A Bar plots displaying the Spearman correlation coefficients between protein-RPF, protein-mRNA, and RPF-mRNA at each developmental stage. B Heat map showing the Spearman correlation coefficients between pairwise protein and RPF among different developmental stages. The black boxes indicate the highly-correlated stages. C Left, violin plot showing the protein levels of all proteins (n = 4,108). Blue dots are proteins that originated from FGOs but were not translated or lowly translated in early embryos (FGO originated, RPF FPKM < 5 at the 1C stage and afterward). Right, line plots show the protein, RPF, and mRNA dynamics of representative genes. D Heat maps showing the parent-of-origin dynamics of proteins in oocytes and early embryos, with the corresponding parent-of-origin mRNA levels mapped. The example genes are also listed. maternal, C57BL/6J; paternal, PWK/PhJ; n, gene number. E Line plots showing the parent-of-origin of proteins and mRNAs across developmental stages for example genes. F Heat map showing the Spearman correlation coefficients between protein changes and RPF changes among different developmental stages. The dashed lines represent synchronized (black), one-stage lagging (red), and two-stage lagging (blue) changes. Arrows indicate the example transition stages that are highly correlated between protein changes and RPF changes