Fig. 3

Global proteome dynamics in mouse oocytes and pre-implantation embryos. A Heat maps showing the K-means clustering results based on protein dynamics in oocytes and early embryos (n = 4,108), with the corresponding RPF and mRNA levels mapped. The enriched GO terms and example genes are also listed. Constitutive proteins are further classified into four subgroups based on the dynamics of RPFs. 1^st subgroup, protein constitutive-RPF constitutive; 2^nd subgroups, protein constitutive-RPF OET downregulated; 3^rd subgroup, protein constitutive-RPF low; 4^th subgroup, protein constitutive-RPF maternal. B Line plots showing the protein, RPF, and mRNA dynamics across stages for genes from different clusters in (A). Left, the representative genes or gene families; right, the average signals for each cluster. Arrows indicate discordant dynamics among proteome (red), translatome (black), and transcriptome (black) datasets