Fig. 2

Distinct global proteome, translatome, and transcriptome dynamics during mouse oocyte maturation and early development. A Schematic of mouse oocytes and preimplantation embryos used for LC–MS/MS, Ribo-seq, and RNA-seq. B Principal component analysis (PCA) of genes (n = 4,108) based on their protein, translation (RPF), and transcription (mRNA) levels. The sample points are colored by developmental stage and the replicates are connected with solid lines. The developmental trajectories of samples are shown in red lines. C Heat maps showing the Spearman correlation coefficients between pairwise developmental stages for protein, RPF, and mRNA. Arrows indicate the stages that show evident transitions for the proteome, translatome, or transcriptome. D Pie charts showing the proportions of stably and dynamically regulated (coefficient of variation > 0.2 of log2 transformed intensity across stages) proteins, RPFs, and mRNAs for detected genes (n = 4,108) in oocytes and early embryos