Fig. 1
Low-input LC–MS/MS efficiently quantifies the protein abundance. A Validation of LC–MS/MS quantification capability. Schematic showing the experimental design of validation with various numbers of FGOs (left). Scatter plots comparing log2 transformed protein intensities of 100, 200, and 500 FGOs against 10 FGOs (right). Lines representing the log2 protein intensity with expected fold changes comparing with that of 10 FGOs are shown in red, and the reference line y = x is shown in black. iBAQ, intensity-based absolute quantification. B Schematic showing the experimental design of the MII oocytes treated with DMSO or CHX for 24 h or 48 h, followed by proteome profiling with LC–MS/MS. C Venn diagram showing the overlap of CHX repressed proteins and dormant mRNAs identified by Ribo-seq (based on ribosome protected fragments, RPF) [12] (RPF, MII oocyte/FGO > 2; mRNA, MII oocyte/FGO < 2). P-value calculated by Fisher’s Exact test is also shown. D Heat maps showing protein fold changes (FC) and protein intensities upon CHX treatment in MII oocytes for known dormant genes. RPF and mRNA levels are also shown. A CHX unaffected gene is shown as a control