Fig. 3

Inferring protein expression from gene expression in single-cell peripheral blood mononuclear cells. A Cell-wise Pearson correlation (y-axis) of inferred and measured protein expression by different methods (x-axis): CMOT (p = 25%, 50%, 75%, 100%), Seurat, MOFA + , TotalVI (Additional File 1: Tables S11-S12). B The measured (x-axis) versus inferred normalized expression (y-axis) of 14 proteins (dots) for two select cells. C Pearson correlations of inferred and measured expression (y-axis) of individual proteins (x-axis) by CMOT (p = 100%, 75%), Seurat, MOFA + , TotalVI (Additional File 1: Table S13). D UMAPs of inferred and measured expressions for three proteins: TIGIT (r = 0.45; p = 6.18e − 197) (top), CD16 (r = 0.75; p = 0) (middle), CD8a (r = 0.55; p = 3.11e − 312) (bottom) (Additional File 1: Table S13). The intensity represents the protein expression level. r is the Pearson correlation coefficient. p is the correlation p-value