Fig. 2

Downregulation of Polq enhances HDR efficiency. A Representative images display the knock-in efficiency at the Actb and Gata6 loci. The ratio of mCherry-positive blastocysts indicates the knock-in efficiency. The “Ctrl” group signifies that no gene is knocked down, while “KD-Rad52,” “KD-Ku70,” “KD-Polq,” and “KD-All” represent the knockdown of Rad52, Ku70, Polq genes, and all three genes, respectively. The scale bar represents 100 μm. B Representative images of 9 genes integrated with mCherry using both baseline and CATI methods. HDR efficiency and the number of blastocysts are displayed. N denotes the number of replicates, and n indicates the total number of blastocysts obtained from all replicates. The scale bar is set at 100 μm. C A statistical analysis of the CATI method’s improvement in HDR efficiency for 11 loci in mouse embryos. A total of 33 experimental pairs were included in the analysis. A two-sided Student’s t-test was used for statistical evaluation, with ***p < 0.001 considered significant. Data are presented as mean ± standard error of the mean (s.e.m.). D A comparative analysis of targeting efficiency (left) and birth rate (right) for Cre or LoxP at 3 loci in mouse offspring, employing both baseline and CATI methods